LOCUS       sequence-215753-        1795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK301
FEATURES             Location/Qualifiers
     source          1..1795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      97..685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     586..605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      808..879
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      895..922
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(946..980)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1051..1710)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1625..1644
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
       61 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
      121 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      181 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      241 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      301 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      361 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      421 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      481 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      541 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      601 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      661 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      721 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      781 ccgtagtcgg tctcatacac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      841 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      901 ccttcgggtg ggcctttctg cgtttatacc gatgagacca gaccaataaa aaacgcccgg
      961 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     1021 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
     1081 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     1141 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     1201 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     1261 ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag
     1321 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     1381 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     1441 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     1501 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     1561 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     1621 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     1681 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     1741 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaa
//
