LOCUS       sequence-215830-        3853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crtI in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::crtI
FEATURES             Location/Qualifiers
     source          1..3853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1787..1873)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1886..1904)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1972..2076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2077..2145,2146..2937)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2295..2314)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3108..3696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3597..3616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtgctcttcg atgacatcag
      121 ctctcccccg cccggcacca agtccgtacg cacgccgtaa aacggcgttg gttattggcg
      181 caggttttgg tgggttggcc ctgggcattc gtctacagtc gttaggtttt gatacaacaa
      241 ttttggaacg tctggatggt cctggtggtc gcgcgtatca aaaacgtacc ccagatggct
      301 atgtctttga catgggtccg actgtgctga cggtgccgca ttttatcgaa gaactgtttg
      361 cgcttgaacg tgatcgtgcc ggcctggatg cccccgatta tcctcctgaa gtgttgtcgg
      421 gcgagcgcgt taaggaaggc gtttctggtg gcccgcatac gagccggtat gtcaccttag
      481 tgccgattct gcccttttac cgcattgttt ttcacgatgg cacgtatttt gattatgatg
      541 gcgaccctga aagtactcgg cggcagattg ctgaattggc ccctggcgac ttagccgggt
      601 atgaacgctt tcatgccgat gccgaggcca tctttcgtcg gggcttcctg gaactgggct
      661 acacgcactt tggtgacgtc ccgacgatgc tgcgggttgt acctgatctg ctcaaactgg
      721 acgccgttcg gaccctgttc tcctttacga gtaagtactt tcagagcgac aaactgcgcc
      781 aagtgttctc ttttgaaacc cttttggtgg gtgggaatcc tctgagtgtg ccggcgatct
      841 atgcaatgat tcacttcgtt gaaaagactt gggggatcca ctatgccatg ggcggcacag
      901 gcgcactggt gcgcggccta gtccaaaaat ttgaggagct gggtggcgcc attcgttatg
      961 gcgccggcgt cgatgaagta ctggtggatg gcaatctgcc tggtaaacgc acagcgcggg
     1021 gtgtgcgcct ggaaagcggc gaagaactgc gcgccgacct ggtggcgtcc aatggcgatt
     1081 gggctaacac gtatctgaaa cgcgtccggc catcggcacg tctggtcaac tctgatttac
     1141 gcgtgaaagc cgcatctgaa agtatgagcc tgctcgtggt ttatttcggg tttcgcggcg
     1201 gtgatgacct gcccctcaaa catcataata ttttattagg cccacgctac gaggctctgc
     1261 tgagcgaaat ctttggcaca aaacggttgg gcgaagattt tagccagtac ctgcacgtcc
     1321 caacgctcac cgatccggct ctggcacccg cgggtcatca tgcggcctat acacttgtcc
     1381 cggtgccgca taatggctcg ggtattgatt gggacgtgga aggtccaaag cttgccgaag
     1441 cagccctggc agatatcgag cgccgcggtt tgattccggg cctccgtgaa cggctcacac
     1501 attttgaatt tattacgcca gattatttcg caggcactct cgattcctat ctggggaacg
     1561 cgtttggtcc ggagccgcgt ctggtccagt cggcattttt ccgcccgcac aaccgcagcg
     1621 aggatctcca caacttttac ttagtcgggg cgggcgcgca gccaggcgca ggcacaccga
     1681 gcgttatgat gtccgcgaaa atgacagcgc gcctaatcgc tgaagatttc ggtatccatg
     1741 ctgatatccg gcgctaatga agagcgtaag acctctaggg cggcggattt gtcctactca
     1801 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
     1861 ttcgttttat ttgatgcccg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1921 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1981 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2041 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     2101 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     2161 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     2221 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     2281 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     2341 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     2401 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     2461 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     2521 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     2581 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     2641 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2701 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2761 attgctgata aatctggagc cggtgagcgt gggtcccgcg gtatcattgc agcactgggg
     2821 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2881 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2941 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3001 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3781 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3841 accgagcgcc ttg
//
