LOCUS       sequence-215831-        3184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crtB in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::crtB
FEATURES             Location/Qualifiers
     source          1..3184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1118..1204)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1217..1235)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1303..1407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1408..1476,1477..2268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1626..1645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2439..3027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2928..2947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtgctcttcg atgcgtagtc
      121 gcgctggtct gagcttacgg ttacccacgc gtaccttgac cgtgaccgat tactcccccg
      181 ccctgccctg caccgaactg cgccgtcctc cactggctca ggcggttcgc tactgtcggg
      241 atttgacccg ccagcactca aagaccttct atctgggttc acagctcttt tcgcctccgg
      301 aacgcgcggc agtttgggca gtgtatgcgg cgtgccgcgc gggcgatgac atcgtcgatg
      361 aagccggcaa cggcgaccgc gaacgcgaat tgcgggaatg gcgcagccgt attgatgccg
      421 cgtttgctgg ccaaccagcg gatgatccca tctcaaccgc gctggcctgg gcggcaggtc
      481 ggtacgccat cccgcactca gctttcgcgg aactgcatga aggcctcaac atggatttac
      541 gcggtcatga ataccgtgat atggatgact tgttactgta ttgccgccgt gtggcaggtg
      601 tggttggctt tatggtggca ccgatttctg gctaccgtgg gggggctgct accctgaatg
      661 atgctctcca actagggcag gcgatgcaac tgacgaatat tctgcgcgat gtcggtgaag
      721 atctgacccg cggccgcgta tacctgccac agtctctgct tgatgaatat ggcctgtctc
      781 gcgccgcgtt agagcgctgg ggtcagggtg agcccctgtc accggcctac cgtgctctca
      841 tgactcatct tggcggcctt gcacgtgaat ggtatgcagc aggtcgtgct ggtattcctc
      901 aacttgatgg acgcggtcct ctcgccgttc tgactgccgc ccgtgcgtat gagggtattc
      961 tggacgattt ggaacgggcc ggctacgaca acttcggtcg gcgcgcgtac gtgtcaggtc
     1021 gtcgtaaact tctgatgtta ccgcaggcct ggtgggaact gcgtagtctg ggcgctgtcc
     1081 acggctaatg aagagcgtaa gacctctagg gcggcggatt tgtcctactc aggagagcgt
     1141 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
     1201 tttgatgccc gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     1261 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1321 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1381 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1441 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1501 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1561 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1621 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1681 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1741 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1801 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1861 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1921 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1981 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2041 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2101 aaatctggag ccggtgagcg tgggtcccgc ggtatcattg cagcactggg gccagatggt
     2161 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2221 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2281 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2341 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2581 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3061 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3121 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3181 cttg
//
