LOCUS       sequence-215972-        5093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa257 to C-terminus of Cre recombinase fused to GAI CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2231_pCAG-PV1-GAI-L1-iCre-257C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     347..369
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     431..450
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             570..587
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1137..1157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1167..1391
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1631..1650)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1696..1751
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1750..1769)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2112..2128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2112..2128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2125..2147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2136..2152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2160..2166,2167..2184,2185..2190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2205..2226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2311..2445
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2465..2599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2502..2521)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2556..2575
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2667..2684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2838..3426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2918..2937)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3597..4388,4389..4457))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4220..4239
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4458..4562)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4593..4972
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc
       61 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
      121 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
      181 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
      241 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
      301 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
      361 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
      421 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgatt
      481 aattcgagcg aaggcgcgcc cattactcgc atccattctc aggctgtctc gtctcgtctc
      541 gcacgcgtgc cgccaccatg aagcgggacc accaccatca ccatcatcag gacaagaaaa
      601 ccatgatgat gaacgaagag gacgacggca acggcatgga cgagctgctg gctgtgctgg
      661 gctacaaagt gcggagcagc gagatggccg acgtggccca gaaactggaa cagctggaag
      721 tgatgatgag caacgtgcag gaagatgacc tgtcccagct ggccaccgag acagtgcact
      781 acaaccccgc cgagctgtac acctggctgg actccatgct gaccgacctg aactccggag
      841 ggtctggctc cggatcaagt ggtggcagcg gtacctccac ccgggccctg gaagggatct
      901 ttgaggccac ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg
      961 cctggtctgg ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt
     1021 ccatccctga aatcatgcag gctggtggct ggaccaatgt gaacattgtg atgaactaca
     1081 tcagaaacct ggactctgag actggggcca tggtgaggct gctcgaggat ggggacccca
     1141 agaaaaagcg gaaggtgtga gaattcctgt gccttctagt tgccagccat ctgttgtttg
     1201 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     1261 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     1321 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     1381 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     1441 gccctgtagc gtgtaccgct gggagttcgt agacggaaac aaacgcagaa tccaagcgcg
     1501 gccgcccagg atacatagat taccacaact ccgagccctt ccaccgctag cgcactcctc
     1561 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     1621 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     1681 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     1741 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     1801 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     1861 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     1921 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     1981 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     2041 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
     2101 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     2161 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2221 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     2281 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc agttccgccc
     2341 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     2401 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     2461 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2521 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2581 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2641 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2701 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2761 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2821 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2881 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2941 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3001 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3061 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3121 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3181 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3241 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3301 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3361 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3421 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3481 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3541 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3601 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3661 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3721 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3781 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3841 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3901 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3961 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4021 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4081 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4141 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4201 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4261 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4321 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4381 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4441 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4501 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4561 cgcacatttc cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata
     4621 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     4681 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     4741 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     4801 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     4861 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     4921 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
     4981 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
     5041 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg ggg
//
