LOCUS       sequence-215978-        6517 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa270 of Cre recombinase fused to GID1 CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6517
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2564..2584
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2594..2818
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3058..3077)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3123..3178
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3177..3196)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3539..3555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3539..3555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3552..3574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3563..3579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3587..3593,3594..3611,3612..3617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3632..3653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3738..3872
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3892..4026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3929..3948)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3983..4002
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4094..4111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4265..4853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4345..4364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5024..5815,5816..5884))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5647..5666
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5885..5989)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6020..6399
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgt ccaacctgct gactgtgcac caaaacctgc ctgccctccc
      721 tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca
      781 ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg
      841 gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga gggactacct
      901 cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc tgggccagct
      961 caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg ctgtgtccct
     1021 ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca agcaggccct
     1081 ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact ctgacagatg
     1141 ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc tgcgcattgc
     1201 cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga gaatgctgat
     1261 ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg ccctgtccct
     1321 gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg atgaccccaa
     1381 caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctg ccacctccca
     1441 actgtccacc cgggccctgg aagggatctt tgaggccacc caccgctccg gagggtctgg
     1501 ctccggatca agtggtggca gcggtaccat ggccgccagc gacgaagtga acctgatcga
     1561 gagcagaacc gtggtgcccc tgaacacctg ggtgctgatc tccaacttca aggtggccta
     1621 caacatcctg cggaggcccg acggcacctt caacagacac ctggccgagt acctggaccg
     1681 gaaagtgacc gccaacgcca accctgtgga cggcgtgttc agcttcgacg tgctgatcga
     1741 ccggcggatc aacctgctga gccgggtgta cagacccgcc tacgccgatc aggaacagcc
     1801 cccctctatc ctggatctgg aaaagcccgt ggatggcgac atcgtgcccg tgatcctgtt
     1861 cttccacggc ggcagctttg cccacagcag cgccaatagc gccatctacg acaccctgtg
     1921 cagacggctc gtgggcctgt gcaaatgcgt ggtggtgtcc gtgaactacc gcagagcccc
     1981 cgagaaccct tacccctgcg cctacgatga tggctggatc gccctgaact gggtcaacag
     2041 cagaagctgg ctgaagtcca agaaagacag caaggtgcac atctttctgg ccggcgatag
     2101 cagcggcggc aatatcgccc ataacgtggc cctgagagcc ggcgagtctg gcatcgatgt
     2161 gctgggcaat atcctgctga accccatgtt cggcggcaac gagcggaccg agagcgagaa
     2221 gtctctggac ggcaagtact tcgtgaccgt gcgggaccgg gactggtact ggaaggcctt
     2281 tctgcccgag ggcgaggaca gagagcaccc cgcctgcaat cccttcagcc ccagaggcaa
     2341 aagcctggaa ggcgtgtcct tcccaaagtc cctggtggtg gtggccggcc tggacctgat
     2401 cagagattgg cagctggcct atgccgaggg cctgaagaaa gccggccagg aagtgaagct
     2461 gatgcacctg gaaaaggcca ccgtgggctt ctacctgctg cccaacaaca accacttcca
     2521 caacgtgatg gacgagatca gcgccttcgt gaacgccgag tgccccaaga aaaagcggaa
     2581 ggtgtgagaa ttcctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2641 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2701 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2761 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     2821 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcgtg
     2881 taccgctggg agttcgtaga cggaaacaaa cgcagaatcc aagcgcggcc gcccaggata
     2941 catagattac cacaactccg agcccttcca ccgctagcgc actcctcagg tgcaggctgc
     3001 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     3061 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     3121 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     3181 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     3241 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     3301 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     3361 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     3421 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     3481 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     3541 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3601 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     3661 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa
     3721 ttagtcagca accatagtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3781 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3841 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     3901 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3961 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4021 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4441 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4681 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4921 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4981 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5041 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5101 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5161 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5221 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5281 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5341 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5401 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5461 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5521 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5581 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5641 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5701 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5761 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5821 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5881 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5941 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6001 gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg
     6061 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     6121 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     6181 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     6241 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     6301 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     6361 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     6421 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     6481 taattatttt gtgcagcgat gggggcgggg ggggggg
//
