LOCUS       sequence-216268-        9722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of SV40 large T antigen and mCherry red 
            fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Holczbauer A, Factor VM, Andersen JB, Marquardt JU, Kleiner DE, 
            Raggi C, Kitade M, Seo D, Akita H, Durkin ME, Thorgeirsson SS
  TITLE     Modeling pathogenesis of primary liver cancer in lineage-specific 
            mouse cell types.
  JOURNAL   Gastroenterology. 2013 Jul;145(1):221-31. doi: 
            10.1053/j.gastro.2013.03.013. Epub 2013 Mar 19.
  PUBMED    23523670
REFERENCE   2  (bases 1 to 9722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.03.013"; journalName: "Gastroenterology"; 
            date: "2013-07"; volume: "145"; issue: "1"; pages: "221-31"
COMMENT     Sequence Label: pRRLsin-SV40 T antigen-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..9722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             99..2225
                     /codon_start=1
                     /product="large T antigen from SV40 (simian virus 40)"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     misc_feature    join(2294..2868,2869..2871,2872..2880)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2461..2478)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2688..2707
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2881..3591
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3026..3044)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3294..3313
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3611..3627)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3611..3627)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    3642..4230
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3695..3715)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4113..4124)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4317..4550
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4622..4743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4659..4678)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4713..4732
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4762..4897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4917..4936)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4918..4936)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4946..4963)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4946..4962)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4955..4977)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5104..5559
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5191..5210)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5401..5422
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5585..5689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5690..5758,5759..6550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5908..5927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6721..7309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7210..7229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7463..7480
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7597..7618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7633..7638,7639..7656,7657..7663)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7671..7687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7676..7698
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7695..7711
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7695..7711
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7730..7750
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7732..7750
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7778..8004
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8005..8185
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8232..8357
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8855..9088
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9273..9317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9584..9701
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgag
       61 gatgaggatc ccgcgaccgg tcgaccagct ttgcaaagat ggataaagtt ttaaacagag
      121 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctgg gggaatattc
      181 ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat aaaggaggag
      241 atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa aatggaagat ggagtaaaat
      301 atgctcatca acctgacttt ggaggcttct gggatgcaac tgagattcca acctatggaa
      361 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
      421 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
      481 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
      541 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
      601 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
      661 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
      721 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
      781 aggaatattt gatgtatagt gccttgacta gagatccatt ttctgttatt gaggaaagtt
      841 tgccaggtgg gttaaaggag catgatttta atccagaaga agcagaggaa actaaacaag
      901 tgtcctggaa gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat
      961 tgcttgggat gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa
     1021 aagaacagcc cagccactat aagtaccatg aaaagcatta tgcaaatgct gctatatttg
     1081 ctgacagcaa aaaccaaaaa accatatgcc aacaggctgt tgatactgtt ttagctaaaa
     1141 agcgggttga tagcctacaa ttaactagag aacaaatgtt aacaaacaga tttaatgatc
     1201 ttttggatag gatggatata atgtttggtt ctacaggctc tgctgacata gaagaatgga
     1261 tggctggagt tgcttggcta cactgtttgt tgcccaaaat ggattcagtg gtgtatgact
     1321 ttttaaaatg catggtgtac aacattccta aaaaaagata ctggctgttt aaaggaccaa
     1381 ttgatagtgg taaaactaca ttagcagctg ctttgcttga attatgtggg gggaaagctt
     1441 taaatgttaa tttgcccttg gacaggctga actttgagct aggagtagct attgaccagt
     1501 ttttagtagt ttttgaggat gtaaagggca ctggagggga gtccagagat ttgccttcag
     1561 gtcagggaat taataacctg gacaatttaa gggattattt ggatggcagt gttaaggtaa
     1621 acttagaaaa gaaacaccta aataaaagaa ctcaaatatt tccccctgga atagtcacca
     1681 tgaatgagta cagtgtgcct aaaacactgc aggccagatt tgtaaaacaa atagatttta
     1741 ggcccaaaga ttatttaaag cattgcctgg aacgcagtga gtttttgtta gaaaagagaa
     1801 taattcaaag tggcattgct ttgcttctta tgttaatttg gtacagacct gtggctgagt
     1861 ttgctcaaag tattcagagc agaattgtgg agtggaaaga gagattggac aaagagttta
     1921 gtttgtcagt gtatcaaaaa atgaagttta atgtggctat gggaattgga gttttagatt
     1981 ggctaagaaa cagtgatgat gatgatgaag acagccagga aaatgctgat aaaaatgaag
     2041 atggtgggga gaagaacatg gaagactcag ggcatgaaac aggcattgat tcacagtccc
     2101 aaggctcatt tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc
     2161 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     2221 cataaaatga atgcaattgt tgttgttaac ggggatcctc atcgcggccg ctacgtaaat
     2281 tccgcccccc ccccccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     2341 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     2401 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     2461 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     2521 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     2581 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     2641 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     2701 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     2761 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     2821 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc
     2881 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     2941 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     3001 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     3061 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     3121 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     3181 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     3241 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     3301 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     3361 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     3421 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     3481 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     3541 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agaattcctg
     3601 cagcccgggg gatccactag ttctagagcg gccgcgtcga caatcaacct ctggattaca
     3661 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3721 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3781 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3841 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3901 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3961 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4021 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     4081 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4141 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4201 gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc
     4261 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4321 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     4381 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4441 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4501 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     4561 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     4621 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4681 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4741 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagtyccgc ccattctccg
     4801 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4861 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     4921 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     4981 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5041 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5101 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5161 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5221 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5281 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5341 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5401 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5461 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5521 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     5581 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5641 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5701 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5761 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5821 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5881 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5941 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6001 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6061 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6121 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6181 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6241 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6301 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6361 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6421 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6481 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6541 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6601 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6661 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6721 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6781 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6841 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6901 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6961 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7021 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7081 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7141 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7201 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7261 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7321 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7381 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7441 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7501 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7561 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7621 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7681 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     7741 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     7801 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     7861 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     7921 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     7981 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     8041 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8101 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8161 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     8221 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     8281 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     8341 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     8401 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     8461 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     8521 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     8581 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     8641 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggcct
     8701 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     8761 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     8821 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     8881 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     8941 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     9001 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9061 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     9121 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     9181 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     9241 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     9301 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9361 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     9421 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     9481 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     9541 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg
     9601 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     9661 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc
     9721 tc
//
