LOCUS       sequence-21676-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Arora T, Liu B, He H, Kim J, Murphy TL, Murphy KM, Modlin RL, Shuai 
            K
  TITLE     PIASx is a transcriptional co-repressor of signal transducer and 
            activator of transcription 4.
  JOURNAL   J Biol Chem. 2003 Jun 13. 278(24):21327-30.
  PUBMED    12716907
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Jun 13. 278(24):21327-30."
COMMENT     Sequence Label: Flag-hPIASx alpha
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..59
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctcgttta gtgaaccgtc agaattgcca ccatggacta caaggacgac gatgacaaga
       61 attctgcagg atccaccatg gcggatttcg aagagttgag gaatatggtt tctagtttta
      121 gggtttctga actacaagta ttactaggct ttgctggacg gaataaaagt ggacgcaagc
      181 atgacctcct gatgagggcg ctgcatttat tgaagagcgg ctgcagccct gcggttcaga
      241 ttaaaatccg agaattgtat agacgccgat atccacgaac tcttgaagga ctttctgatt
      301 tatccacaat caaatcatcg gttttcagtt tggatggtgg ctcatcacct gtagaacctg
      361 acttggccgt ggctggaatc cactcgttgc cttccacttc agttacacct cactcaccat
      421 cctctcctgt tggttctgtg ctgcttcaag atactaagcc cacatttgag atgcagcagc
      481 catctccccc aattcctcct gtccatcctg atgtgcagtt aaaaaatctg cccttttatg
      541 atgtccttga tgttctcatc aagcccacga gtttagttca aagcagtatt cagcgatttc
      601 aagagaagtt ttttattttt gctttgacac ctcaacaagt tagagagata tgcatatcca
      661 gggatttttt gccaggtggt aggagagatt atacagtcca agttcagttg agactttgcc
      721 tggcagagac aagt
//
