LOCUS       sequence-216904-       10832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IKZF5-3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 10832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UBC- IKZF5-3xHA-pGK-PUR
FEATURES             Location/Qualifiers
     source          1..10832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        78..1289
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1126..1145
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1337..1361
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2622..2646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2693..2782
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        2824..3323
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2859..2882)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3256..3275
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3344..3943
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3344..3363)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3840..3860
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(3974..4007)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4063..4651
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4116..4136)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4534..4545)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4654..4670)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4655..4671)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4860..5093
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5165..5286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5202..5221)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5256..5275
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5326..5461
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5481..5500)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5482..5500)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5523..5881)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5563..5584)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5775..5794
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5908..6012
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6014..6371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6014..6205
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6037..6057)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6222..6357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6406..7200
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6406..7200
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6460..6479)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7070..7089
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7388..7407)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7432..7479
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7808..8396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8297..8316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8571..8874
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8876..9074
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9029..9049
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9092..9272
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9319..9444
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9941..10174
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10359..10403
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10701..10818
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggacagcaga gatccagttt ggttagtacc gggcccgctc tagccctaga tttaacccaa
       61 cagaaggctc gacgcgtggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
      121 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
      181 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
      241 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
      301 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
      361 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
      421 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag
      481 tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa
      541 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact
      601 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct
      661 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
      721 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
      781 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
      841 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
      901 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
      961 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     1021 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     1081 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     1141 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     1201 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     1261 tctggccgtt tttggctttt ttgttagacg ctagactgcc ggatcgaatt gtcgacgaat
     1321 tcggatctgg ccaatcacaa gtttgtacaa aaaagttggc atgggtgaaa aaaaaccaga
     1381 gcctttggac ttcgtgaaag attttcagga atacctgact cagcagaccc atcacgtgaa
     1441 catgatttct ggatcagtta gtggggacaa agaagcagag gctcttcagg gagctggaac
     1501 agatggtgat caaaatggac ttgatcaccc atctgttgaa gtttccttgg atgaaaactc
     1561 aggaatgtta gtagacgggt ttgaaaggac ctttgatggg aagcttaagt gtcggtactg
     1621 caactatgcc agcaaaggaa cagcccggct tattgaacac atcagaatcc acacaggtga
     1681 aaaacctcat cgatgtcatc tttgtccatt tgcatctgct tatgagcgtc atctggaagc
     1741 ccatatgcgt tctcatactg gagaaaaacc atacaaatgt gaattatgtt ccttccgctg
     1801 cagtgatcga agtaacttgt cccatcatcg aaggcgcaag cataaaatgg taccaattaa
     1861 aggtactagg tcttccttaa gcagcaagaa aatgtggggg gttttacaga agaaaacaag
     1921 caatctgggc tatagcagaa gagcactaat caacttaagt ccaccttccg tggtggttca
     1981 gaaaccagac taccttaacg attttaccca cgaaatccca aatatccaga ctgactccta
     2041 tgaaagtatg gcaaaaacca caccaactgg tggccttcca agggaccccc aagaactcat
     2101 ggttgataac cctttgaatc agctctcgac tctagcaggg cagttgtcca gtctgccacc
     2161 cgaaaaccaa aaccctgcat cacctgatgt agttccctgc cctgatgaaa agcctttcat
     2221 gattcagcag ccctctaccc aagcagtagt ttctgccgta tcagcaagta ttcctcagag
     2281 ctcctctccc acaagcccag aacctcggcc atcccatagt caaaggaact atagtccagt
     2341 ggcaggtcca agcagtgagc caagtgccca cacgagcact cccagcatag gaaacagcca
     2401 gccaagcacc ccagccccag ccctgccggt ccaggaccct cagcttctgc accactgcca
     2461 gcactgtgat atgtactttg cagacaacat cctttacact attcatatgg gatgtcatgg
     2521 gtatgaaaat ccttttcagt gtaatatatg tggatgcaaa tgtaaaaaca agtatgattt
     2581 tgcctgtcat tttgcaagag ggcacataac caacattgat tgcccaactt tcttgtacaa
     2641 agtggtgggt ggaggcggtt caggtggcgg aggaagcgga ggcggtggaa gttaccccta
     2701 cgacgtgccc gactacgccg gctatccgta tgatgtcccg gactatgcag gatcctatcc
     2761 atacgacgtt ccagattacg cttagttaat taaggccggc cctcgaggtt aacgaattct
     2821 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     2881 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     2941 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     3001 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     3061 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     3121 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     3181 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     3241 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     3301 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     3361 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     3421 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     3481 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     3541 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     3601 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     3661 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     3721 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     3781 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     3841 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     3901 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgatgtacaa gtaggaattc
     3961 gtcgagggac ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg
     4021 ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac
     4081 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4141 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4201 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4261 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4321 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4381 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4441 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4501 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4561 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4621 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     4681 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     4741 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     4801 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4861 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     4921 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4981 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5041 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     5101 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     5161 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5221 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5281 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     5341 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5401 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5461 ctagggacgt acccaattcg ccctatagtg agtcgtatta gcatgtgagc aaaaggccag
     5521 cataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     5581 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     5641 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5701 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5761 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     5821 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     5881 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5941 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6001 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     6061 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6121 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6181 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6241 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6301 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6361 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6421 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6481 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6541 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     6601 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6661 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6721 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6781 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6841 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6901 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     6961 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     7021 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     7081 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     7141 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7201 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7261 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7321 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     7381 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     7441 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     7501 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     7561 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     7621 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     7681 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7741 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7801 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7861 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7921 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7981 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8041 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8101 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8161 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8221 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8281 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8341 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8401 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8461 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
     8521 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     8581 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     8641 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     8701 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     8761 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     8821 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     8881 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     8941 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     9001 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     9061 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     9121 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9181 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9241 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     9301 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     9361 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     9421 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     9481 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     9541 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     9601 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     9661 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     9721 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     9781 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     9841 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     9901 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     9961 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
    10021 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
    10081 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
    10141 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
    10201 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
    10261 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
    10321 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
    10381 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
    10441 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
    10501 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
    10561 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
    10621 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
    10681 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
    10741 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
    10801 acaaaaattc aaaattttcg ggtttattac ag
//
