LOCUS       sequence-217002-         279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses IMX-DogTag in bacterial cells for modular antigen 
            multimerization using SnoopLigase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Andersson AC, Buldun CM, Pattinson DJ, Draper SJ, Howarth M
  TITLE     SnoopLigase peptide-peptide conjugation enables modular vaccine 
            assembly.
  JOURNAL   Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.1038/s41598-019-40985-w.
  PUBMED    30874593
REFERENCE   2  (bases 1 to 279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-03-15"; volume: "9(1):4625. doi"; pages: " 
            10.1038/s41598-019-40985-w"
COMMENT     Sequence Label: pET28a-IMX-DogTag
FEATURES             Location/Qualifiers
     source          1..279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             205..273
                     /codon_start=1
                     /product="peptide derived from the RrgA adhesin of 
                     Streptococcus pneumoniae"
                     /label=DogTag
                     /note="forms a covalent bond with the DogCatcher protein 
                     when inserted into an intra-domain protein loop (Keeble et 
                     al., 2022)"
                     /translation="DIPATYEFTDGKHYITNEPIPPK"
ORIGIN
        1 atgggcagca gcaaaaaaca aggtgatgca gatgtttgtg gcgaagtcgc ctatatccaa
       61 tccgttgtta gtgattgtca cgtaccaact gccgaattgc gtaccctttt ggagatccgt
      121 aagctgtttc ttgagattca aaaactgaaa gttgaattac agggtcttag caaggaaggt
      181 tccggatccg gtgaaggttc cggtgatatt ccggctacat atgaatttac cgatggtaaa
      241 cattatatca ccaatgaacc gataccgccg aaataataa
//
