LOCUS       sequence-217065-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked
            with URA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Costa EA, Subramanian K, Nunnari J, Weissman JS
  TITLE     Defining the physiological role of SRP in protein-targeting 
            efficiency and specificity.
  JOURNAL   Science. 2018 Jan 18. pii: science.aar3607. doi: 
            10.1126/science.aar3607.
  PUBMED    29348368
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Jan 18. pii: science.aar3607. doi: 10.1126/science.aar3607."
COMMENT     Sequence Label: pJW1672
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..767)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(54..767)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(792..812)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(819..860)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aataaaaatc ataaatcata agaaattcgc ttatttagaa gtggcgcgcc ttatttgtac
       61 aattcatcca taccatgggt aataccagca gcagtaacaa attctaacaa gaccatgtgg
      121 tctctctttt cgtttggatc tttggataag gcagattgag tggataagta atggttgtct
      181 ggtaacaaga ctggaccatc accaattgga gtattttgtt gataatggtc agctaattga
      241 acagaaccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgatacc attcttttgt
      301 ttgtcagcca tgatgtaaac attgtgagag ttatagttgt attccaattt gtgacctaaa
      361 atgttaccat cttctttaaa atcaatacct tttaattcga ttctattaac taaggtatca
      421 ccttcaaact tgacttcagc tctggtcttg tagttaccgt catctttgaa aaaaatagtt
      481 ctttcttgaa cataaccttc tggcatggca gacttgaaaa agtcatgttg tttcatatga
      541 tctgggtatc tagaaaaaca ttgaacacca taagttaaag tagtgactaa ggttggccat
      601 ggaactggca atttaccagt agtacaaata aattttaagg tcaatttacc gtaagtagca
      661 tcaccttcac cttcaccgga gacagaaaat ttgtgaccat taacatcacc atctaattca
      721 accaaaattg ggacaacacc agtgaataat tcttcacctt tagacatgtt aattaaacca
      781 gcaccgtcac ctccttgaaa atacaaattt tctgaacccg tagaatcgag accgaggaga
      841 gggttaggga taggcttacc cattatagac atgatgattg ccgtcaaaga ccgaccaaag
      901 gggttgtttg g
//
