LOCUS       sequence-217075-       11337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer plasmid for expression of SAD B19 G and 
            mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lavin TK, Matsuyama M, Fu X, Lea NE, Xu 
            R, Hou Y, Rutigliani L, Pruner M, Babcock KR, Ip JPK, Hu M, Daigle 
            TL, Zeng H, Sur M, Feng G, Wickersham IR
  TITLE     Long-term labeling and imaging of synaptically connected neuronal 
            networks in vivo using double-deletion-mutant rabies viruses.
  JOURNAL   Nat Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8.
  PUBMED    38212587
REFERENCE   2  (bases 1 to 11337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8."
COMMENT     Sequence Label: pLV-TTBG
FEATURES             Location/Qualifiers
     source          1..11337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2547..2565,2566..2588,2589..2607,2608..2630,2631..2649,
                     2650..2672,2673..2691,2692..2714,2715..2733,2734..2756,
                     2757..2775,2776..2798,2799..2817)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2850..2874
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2960..3580
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             3734..3790
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3791..4489
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4499..4555
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    6140..6728
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6193..6213)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6611..6622)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7138..7318
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7344..7361)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7350..7574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7620..8048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7707..7726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7917..7938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8057..8077)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8062..8392
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8243..8378
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8305..8324
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8440..8487
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8506..8880
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9010..9131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9047..9066)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9101..9120
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9180..9196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9180..9196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9193..9215)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9204..9220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9228..9234,9235..9252,9253..9258))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9273..9294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9411..9428)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9582..10170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9662..9681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10341..11132,11133..11201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10964..10983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11202..11306)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcgt tggctcgacc
     2521 cgggtaccga gctcgacttt cacttttctc tatcactgat agggagtggt aaactcgact
     2581 ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac
     2641 tgatagggag tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac
     2701 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
     2761 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
     2821 gtaaaggatc cctcgaccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     2881 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc
     2941 ggccctgcag gccgccacca tgtccagact ggacaagagc aaagtcatca actctgcctt
     3001 ggagctcctg aatgaagttg gcattgaggg cttgaccacc aggaagctgg cccagaagct
     3061 gggtgtggag cagcctaccc tgtactggca tgtgaagaac aagagggctc tgcttgatgc
     3121 cctggccatt gagatgttgg acaggcacca cacccacttc tgccctctgg aaggggagtc
     3181 ctggcaggac ttcctgagga acaatgccaa gagcttcaga tgtgccttgc tctcccaccg
     3241 ggatggtgcc aaagttcact tgggcaccag gcctacagag aagcagtatg agaccctgga
     3301 gaaccagctg gcattcctgt gccaacaagg cttctccctg gaaaatgcct tgtatgccct
     3361 ctctgctgtg ggccacttca ccttgggctg tgtgctggag gaccaggagc accaagttgc
     3421 caaggaggag agggagaccc ccaccactga ctccatgcca ccactgctgc ggcaagctat
     3481 tgagttgttt gaccaccaag gggctgagcc tgcattcctt tttggcctgg aactgatcat
     3541 ctgtggcctg gaaaagcagc tgaaatgtga gtctggctct cacgtgccca aaaagagaaa
     3601 gcacgtgcct gctgatgcct tggatgattt tgacctggac atgctgcctg ctgatgccct
     3661 ggatgacttt gatttggaca tgctccctgc tgatgcactt gatgattttg acctggatat
     3721 gctgggaagc ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa
     3781 ccctggacct atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     3841 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     3901 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     3961 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
     4021 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     4081 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     4141 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
     4201 actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag
     4261 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac
     4321 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
     4381 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc
     4441 agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc
     4501 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatggt
     4561 tcctcaggct ctcctgtttg taccccttct ggtttttcca ttgtgttttg ggaaattccc
     4621 tatttacacg ataccagaca agcttggtcc ctggagtccg attgacatac atcacctcag
     4681 ctgcccaaac aatttggtag tggaggacga aggatgcacc aacctgtcag ggttctccta
     4741 catggaactt aaagttggat acatcttagc cataaaagtg aacgggttca cttgcacagg
     4801 cgttgtgacg gaggctgaaa cctacactaa cttcgttggt tatgtcacaa ccacgttcaa
     4861 aagaaagcat ttccgcccaa caccagatgc atgtagagcc gcgtacaact ggaagatggc
     4921 cggtgacccc agatatgaag agtctctaca caatccgtac cctgactacc gctggcttcg
     4981 aactgtaaaa accaccaagg agtctctcgt tatcatatct ccaagtgtgg cagatttgga
     5041 cccatatgac agatcccttc actcgagggt cttccctagc gggaagtgct caggagtagc
     5101 ggtgtcttct acctactgct ccactaacca cgattacacc atttggatgc ccgagaatcc
     5161 gagactaggg atgtcttgtg acatttttac caatagtaga gggaagagag catccaaagg
     5221 gagtgagact tgcggctttg tagatgaaag aggcctatat aagtctttaa aaggagcatg
     5281 caaactcaag ttatgtggag ttctaggact tagacttatg gatggaacat gggtctcgat
     5341 gcaaacatca aatgaaacca aatggtgccc tcccgataag ttggtgaacc tgcacgactt
     5401 tcgctcagac gaaattgagc accttgttgt agaggagttg gtcaggaaga gagaggagtg
     5461 tctggatgca ctagagtcca tcatgacaac caagtcagtg agtttcagac gtctcagtca
     5521 tttaagaaaa cttgtccctg ggtttggaaa agcatatacc atattcaaca agaccttgat
     5581 ggaagccgat gctcactaca agtcagtcag aacttggaat gagatcctcc cttcaaaagg
     5641 gtgtttaaga gttgggggga ggtgtcatcc tcatgtgaac ggggtgtttt tcaatggtat
     5701 aatattagga cctgacggca atgtcttaat cccagagatg caatcatccc tcctccagca
     5761 acatatggag ttgttggaat cctcggttat cccccttgtg caccccctgg cagacccgtc
     5821 taccgttttc aaggacggtg acgaggctga ggattttgtt gaagttcacc ttcccgatgt
     5881 gcacaatcag gtctcaggag ttgacttggg tctcccgaac tgggggaagt atgtattact
     5941 gagtgcaggg gccctgactg ccttgatgtt gataattttc ctgatgacat gttgtagaag
     6001 agtcaatcga tcagaaccta cgcaacacaa tctcagaggg acagggaggg aggtgtcagt
     6061 cactccccaa agcgggaaga tcatatcttc atgggaatca cacaagagtg ggggtgagac
     6121 cagactgtaa tgagctagca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6181 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6241 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6301 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6361 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6421 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6481 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     6541 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6601 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6661 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6721 ctccccgcct gttcgaattc gagctcggta cctttaagac caatgactta caaggcagct
     6781 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6841 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattga
     6901 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     6961 ctagtaccag ttgagccaga taagatagaa gaggccaata aaggagagaa caccagcttg
     7021 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     7081 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga ctgtactggg
     7141 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7201 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7261 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     7321 gccgctttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     7381 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7441 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7501 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     7561 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     7621 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7681 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7741 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag
     7801 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     7861 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7921 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     7981 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8041 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     8101 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8161 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8221 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8281 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8341 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8401 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     8461 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     8521 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8581 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     8641 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     8701 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     8761 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     8821 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     8881 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     8941 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9001 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9061 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9121 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9181 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9241 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9301 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9361 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9421 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9481 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9541 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9601 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9661 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9721 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     9781 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9841 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9901 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9961 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10021 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10081 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10141 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10201 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10261 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10321 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10381 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10441 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10501 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10561 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10621 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10681 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10741 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10801 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10861 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10921 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10981 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11041 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11101 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11161 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11221 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11281 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
