LOCUS       sequence-217093-        8689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prp16 in pSE358(Trp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Burgess SM, Guthrie C
  TITLE     A mechanism to enhance mRNA splicing fidelity: the RNA-dependent 
            ATPase Prp16 governs usage of a discard pathway for aberrant lariat 
            intermediates.
  JOURNAL   Cell. 1993 Jul 2;73(7):1377-91.
  PUBMED    8324826
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1993-07-2"; volume: "73(7)"; pages: "1377-91"
COMMENT     Sequence Label: pSB58 (Prp16)
FEATURES             Location/Qualifiers
     source          1..8689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    66..87
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(102..107,108..125,126..132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    140..156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     145..167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     164..180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     164..180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4748..4765)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4748..4764)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4757..4779)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5279..5571
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      5572..6025
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(5864..6538)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(6539..6640)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(6657..6675)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6743..6847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6848..6916,6917..7708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7066..7085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7879..8467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8368..8387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8621..8638
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
       61 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
      121 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
      181 atgattacga attcgagctc ggtacccggg ctcccattag cagcagcaat tggcttcttt
      241 ggtgtcttct tcttcttcaa agttttcttc aattccgcca gcgtgctttc caaatcgaaa
      301 ttgttgtcaa acaaagctag cttaagtgaa agattatccc agccttggta atcctgtaac
      361 tgcgccttta gagtaggaaa tacctcgttc attagatcat attcatcgtc attcaaataa
      421 tcatcaaatt caccttcgtc gtgaaagtcc ggcatgtcgt ctgctccatc gctgtagtca
      481 ctgtaagcca tctcgatagt caatagttgt cgaagcagaa gcctttgggt gtctccaagc
      541 tgtacttaac tctttttaaa ttgcttattg gttagctttc atgtggtata ttcaagtcac
      601 attaatgggc gtctccgtgc attgtgatca atatatgaat acgaagtact tatagagtag
      661 tatttacacg attgttatta tatttatata gcattcacgc ctggtaccac aggagttttc
      721 tacgcttacg gtcctctctg gctatacgcc tcttctcctt ccagcgtgat tggatttctt
      781 gcaatctact ttccataatt tgtacatgtt gttcactagg tttcgagacc atggatacaa
      841 ataatggcga aatgccatat tttgcagcca aaaatttgcg tgtatacttt tgaggattgt
      901 caagacgtaa cttccggatc tcatcaattt cttgaggtcc cagatgatag gttttgccgt
      961 tcactgtgct cttacctacg agaacatttg gacactgctt cgctttagtg ctttgaacag
     1021 gcatgtcaag ccctctacga gggtcactgg ctgccataaa gctacgtgga atggtctcac
     1081 tgttaacaga tccggttgcg atggattctg atggttgata gtacattcct ggagcatctc
     1141 tgtgctttaa atacccttta tagttagatg cgctcttagt cttgttgtaa atcgttgtca
     1201 cttgtgtttt gggaaatcca aattgcttcc aggcagatcc agcagtgtgg aacgatctgc
     1261 agcacacacc tctgccaatc attcttgtgc tattttactt tgtctttaat tttccatttt
     1321 aacttgttga aagtatctct ttttgccctc ttttctattt tctcaaataa taaaagcggt
     1381 gaaagctgaa aaattcatga gatgactgca gatgataaaa cacaagaagg cccaaagaca
     1441 atacaaatac ctctggtatg ggtcattcgg ggcgtgagga aaggataaaa gacatattta
     1501 aggaacttac gtcaaaagaa ctaacgcctg ggttgctttt gacactgcag aaactagcgc
     1561 aaaagccgaa tactaatcta gaacagttta ttgccagctg taaagctttg acgaaactca
     1621 gcagtaacaa tccaatcatt tttaatgaac ttctggagct cctcaagaat aaatctgaag
     1681 aggactcgac aggcccaaag aaaatcgccc cttctataaa taagagaaag aaatttaaga
     1741 tccagttaga ccttgacgat aatgaagatg agctagattc tccagttcaa aaaaaaccag
     1801 cacctacacg gacgttgttc aagagaatag ataaactaaa ggccaaacag ttgaggcagt
     1861 attcgcccac cgtgaaggat ccttctccaa atagtgaaca gcaaacacag aatggtcatg
     1921 ctgaaacgaa ggactatgaa ccgactcgaa gcgaagtagt tgaggaggac agagaatggt
     1981 atgataatga tgatgattat ggaaatttgg tgcctgaacc tctgtctgaa ttgcctgagg
     2041 aagcgaaact actacctgtc attaggaata tcgacaacga tgatgcctta cgaaataccg
     2101 ttcaattata ccccatacca ctcaagcaaa gaatggaatg gatccctcct tttttatcaa
     2161 agtttgctct tgaaaacaaa gtgcccactt ccataattat cgggtccatc tctgagacgt
     2221 ccagccaagt atccgcttta tcaatggtga atccatttag gaatcctgac agtgaatttt
     2281 ctgcgaacgc caaacgaggt agtaagctgg ttgccttgag aaggataaac atggaacata
     2341 ttcaacaatc tcgagacaac accactgttt tgaacactgc catgggggaa gtactaggct
     2401 tggagaataa caataaagca aaagataaga gcaaccaaaa gatttgcgac gatacagctc
     2461 ttttcacgcc atcaaaagat gacattaaac atactaaaga gcaactgcct gttttccgct
     2521 gcagatctca attgttatca ttgataagag aaaatcaagt agtggtgata attggtgaaa
     2581 cgggctcagg taaaaccacg caacttgcac agtatttata tgaagaagga tatgccaacg
     2641 atagggggaa atctattgtt gtcacacagc cgagaagagt agcagccata tccgttgcaa
     2701 aaagggttgc aatggaaatg caagttccat tgggtaagga agtaggctat tctataaggt
     2761 ttgaagatgt gacggattct gagtgtacaa aacttaagtt tgtaacggat ggtattctac
     2821 tcagagagac cttattggat gatacactcg ataaatattc gtgtgttatt attgatgaag
     2881 ctcatgaaag gtcattaaat acagacattt tactaggttt tttcaagatt ttattagcac
     2941 gtagaagaga tctaaaactc ataatcactt cagcaacaat gaatgcgaag aagttctccg
     3001 cattttttgg taatgctcct cagttcacaa ttcctggaag aactttcccc gtacagacca
     3061 tatatacttc caaccctgtt caagactacg tagaagctgc agtttctcag gctgtgaaaa
     3121 ttcatttagc aaacgattgt tcaagcggtg atattctcat tttcatgaca ggtcaagaag
     3181 atattgaaac aacgttcgac actctgcaag aaaagttttt gcaagtatat tctaaaaagt
     3241 ttggtaccgc aaactttgaa gagatcaatg atattgaaat tttaccaatc tactcggctt
     3301 tacctgcaga tttgcaattc aaaatttttc aggatctaca tggcactaaa aggaaaatta
     3361 taattgcgac aaatattgca gagacttcat tgaccattaa aggtattaga tatgtcattg
     3421 attgtggtta ctctaaactg aaagtttaca atccaaagat tgggcttgat agtttagtaa
     3481 taacgcccat atcaaaagct aacgccgacc aaagatccgg aagagcggga agaactgcgc
     3541 caggcactgc atatcgatta tacacggaag atacattcaa agaagatatg tacttacaga
     3601 caatacctga gatccaaaga acgaatttgt caaacaccct tttactgcta aaatctctgg
     3661 acgttactga cgaattgagt aagttcccgt tcattgataa accaccctta caaacatttt
     3721 tatcctcatt gtatgagtta tggttcatag gggctataga cactagtgga caattgactc
     3781 cattgggatt acagatggca aaatttcccc tgcaaccttc cctttccaaa attttattaa
     3841 ttgcagttag aaacggctgc agtgatgaaa tgttaacaat agtttccatg ctttctgttc
     3901 cacaagtctt ttatagaccc aaggaaagac agaaagaagc agatattgct agaaataaat
     3961 tttttatcgc gaaatcagat cacttaacgt tacttaatgt ttttgaacaa tggagggcaa
     4021 ataattttag ttcgcattgg tgtaacaaac attttgttca atacaaatca cttgtcaggg
     4081 caagagacat tcgagaccag ttgctaacaa tactaaaatc tcaaaaaatt ccggtcattt
     4141 cctccggtaa ggactgggat attattaaaa aatgtatttg ttcaggattt gctcatcaag
     4201 cagccaaaat aacaggacta cgaaattacg ttcatttaaa aactggcgta agtgttcaac
     4261 tacaccctac aagtgcatta cacggattgg gggatttacc cccttacgtg gtatatcatg
     4321 aattattaat gactagcaaa gaatacattt gttgtgttac ttctgtagat ccattctggt
     4381 taatggaata tggtgggtta ctctacgaca tcaagcgtat taaaaatgat caggaggcca
     4441 caactacagg cctctttggt gaacactatg aacatactct ggacaaggtt gaagatgata
     4501 ttgatatcaa tattaggaga tgtaaggata tgagagacag tgtgatacag gagctcaaaa
     4561 tgaccgacaa ttcaaacaaa gaagacaaaa agcaaaaaac gaaaaagcaa aatatactga
     4621 acggcaaaga aaattcaatg aaacctttca aaagaaggaa gccttttttt tagtttatgg
     4681 gaagagcgtg ctaataggca aaatattcat atatgttatt atatagttat atgcatgcaa
     4741 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4801 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4861 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     4921 ttctccttac gcatctgtgc ggtatttcac accgcataac tagcttttaa cttgtatcct
     4981 aggttatcta tgctgtctca ccatagagaa tattacctat ttcagaatgt atgtccatga
     5041 ttcgccgggt aaatacatat aatacacaaa tctggcttaa taaagtctat aatatatctc
     5101 ataaagaagt gctaaattgg ctagtgctat atatttttaa gaaaatttct tttgactaag
     5161 tccatatcga ctttgtaaaa gttcacatta gcatacatat attacacgag ccagaaatag
     5221 taacttttgc ctaaatcaca aattgcaaaa tttaattgct tgcaaaaggt cacatgctta
     5281 taatcaactt ttttaaaaat ttaaaatact tttttatttt ttatttttaa acataaatga
     5341 aataatttat ttattgttta tgattaccga aacataaaac ctgctcaaga aaaagaaact
     5401 gttttgtcct tggaaaaaaa gcactaccta ggagcggcca aaatgccgag gctttcatag
     5461 cttaaactct ttacagaaaa taggcattat agatcagttc gagttttctt attcttcctt
     5521 ccggttttat cgtcacagtt ttacagtaaa taagtatcac ctcttagagt tggcggttgt
     5581 ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg tagcgtatgc gcctgtgaac
     5641 attctcttca acaagtttga ttccattgcg gtgaaatggt aaaagtcaac cccctgcgat
     5701 gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt tagcattatc tttacatctt
     5761 gttattttac agattttatg tttagatctt ttatgcttgc ttttcaaaag gcctgcaggc
     5821 aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc ttagcatttt
     5881 tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta
     5941 catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc
     6001 caccagctaa cataaaatgt aagctttcgg ggctctcttg ccttccaacc cagtcagaaa
     6061 tcgagttcca atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac aagggaataa
     6121 acgaatgagg tttctgtgaa gctgcactga gtagtatgtt gcagtctttt ggaaatacga
     6181 gtcttttaat aactggcaaa ccgaggaact cttggtattc ttgccacgac tcatctccat
     6241 gcagttggac gatatcaatg ccgtaatcat tgaccagagc caaaacatcc tccttaggtt
     6301 gattacgaaa cacgccaacc aagtatttcg gagtgcctga actattttta tatgctttta
     6361 caagacttga aattttcctt gcaataaccg ggtcaattgt tctctttcta ttgggcacac
     6421 atataatacc cagcaagtca gcatcggaat ctagagcaca ttctgcggcc tctgtgctct
     6481 gcaagccgca aactttcacc aatggaccag aactacctgt gaaattaata acagacatac
     6541 tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt gctcaatagt caccaatgcc
     6601 ctccctcttg gccctctcct tttctttttt cgaccgaatt tcttgaagac gaaagggcct
     6661 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     6721 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6781 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6841 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6901 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6961 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7021 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7081 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7141 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7201 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7261 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7321 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7381 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7441 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7501 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7561 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7621 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7681 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7741 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7801 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7861 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7921 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7981 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8041 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8101 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8161 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8221 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8281 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8341 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8401 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8461 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8521 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8581 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8641 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccg
//
