LOCUS       sequence-21734-e         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE
  TITLE     A UbcH5/ubiquitin noncovalent complex is required for processive 
            BRCA1-directed ubiquitination.
  JOURNAL   Mol Cell. 2006 Mar 17. 21(6):873-80.
  PUBMED    16543155
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Mar 17. 21(6):873-80."
COMMENT     Sequence Label: UbcH5c S22R pET28a
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             529..546
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(599..617)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      613..661
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctctg aatattttgt ttaactttaa gaaggagata taccatggcg ctgaaacgga
       61 ttaataagga acttagtgat ttggcccgtg accctccagc acaatgtcgt gcaggtccag
      121 ttggggatga tatgtttcat tggcaagcca caattatggg acctaatgac agcccatatc
      181 aaggcggtgt attctttttg acaattcatt ttcctacaga ctaccccttc aaaccaccta
      241 aggttgcatt tacaacaaga atttatcatc caaatattaa cagtaatggc agcatttgtc
      301 tcgatattct aagatcacag tggtcgcctg ctttaacaat ttctaaagtt cttttatcca
      361 tttgttcact gctatgtgat ccaaacccag atgaccccct agtgccagag attgcacgga
      421 tctataaaac agacagagat aagtacaaca gaatatctcg ggaatggact cagaagtatg
      481 ccatgtgatg ctaccttaaa gtcagaataa cctgcattat agctcgagca ccaccaccac
      541 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      601 gctgagcaat aactagcata accccttggg gcctctaaac ggggtcttga ggggtttttt
      661 gctg
//
