LOCUS       sequence-21735-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE
  TITLE     A UbcH5/ubiquitin noncovalent complex is required for processive 
            BRCA1-directed ubiquitination.
  JOURNAL   Mol Cell. 2006 Mar 17. 21(6):873-80.
  PUBMED    16543155
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Mar 17. 21(6):873-80."
COMMENT     Sequence Label: BRCA1 (1-304)
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..36
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..106
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             107..127
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctagaaaata aattttgttt aactttaaga aggagatata catatgcatc atcaccatca
       61 ccatggtaag cctatcccta accctctcct cggtctcgat tctacggaaa acctgtattt
      121 tcagggaatt gatcccttca ccatggattt atctgctctt cgcgttgaag aagtacaaaa
      181 tgtcattaat gctatgcaga aaatcttaga gtgtcccatc tgtctggagt tgatcaagga
      241 acctgtctcc acaaagtgtg accacatatt ttgcaaattt tgcatgctga aacttctcaa
      301 ccagaagaaa gggccttcac agtgtccttt atgtaagaat gatataacca aaaggagcct
      361 acaagaaagt acgagattta gtcaacttgt tgaagagcta ttgaaaatca tttgtgcttt
      421 tcagcttgac acaggtttgg agtatgcaaa cagctataat tttgcaaaaa aggaaaataa
      481 ctctcctgaa catctaaaag atgaagtttc tatcatccaa agtatgggct acagaaaccg
      541 tgccaaaaga cttctacaga gtgaacccga aaatccttcc ttgcaggaaa ccagtctcag
      601 tgtccaactc tctaaccttg gaactgtgag aactctgagg acaaagcagc ggaatacaac
      661 ctcaaagacg tctgtctaca ttgaattggg atctgattct tctgaagata ccgttaa
//
