LOCUS       sequence-217515-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pLX317-hcRED
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(143..184)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    192..216
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctttccaaa ttttgtaatc cagaggttga ttgtcgactt aacgcgtggg acctgtacgg
       61 tggtgatagc atcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa
      121 gttacggact agttcattac tacgtagaat cgagaccgag gagagggtta gggataggct
      181 taccgatatc aaccactttg tacaagaaag ttggcaagtt ggccttctcg ggcaggtcgc
      241 tgtaccgggc cacgctggcc tcgtacagct cgaagtactc gtccttcttc ttccgcagca
      301 tctggagccg gatgtcggtg aagtggaagc cgggcatggt cagggcgcgc acggccttct
      361 tgctccggta gctggtgtag tggtggcaga tcaggtgccg gtcgcccacc ttcagggcca
      421 tcacgttccg gccgcacagc acgccgttct cggggtacac cacctcggtg ctgggctccc
      481 agccgccgct cttgttcttc atcacggggc cgtcggcggg gaagttggtg ccgtgcacct
      541 tcaccttgta gatcaggcag ttgccctcca ggctggtgtc ctggtgggcg gtcaggatgc
      601 cgccgtcctc gtaggtggtg gttctctccc aggtgaagcc ctcggggaag ctctgcttga
      661 agaagtcggg gatctcggcg gtgtggtgca cgaaggtcct gctgccgtac tcgcagca
//
