LOCUS       sequence-217521-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pLX317-MBNL1
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(143..184)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    192..216
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctttccaaa ttttgtaatc cagaggttga ttgtcgactt aacgcgtggt ctgttgccga
       61 gtaccttcac atcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa
      121 gttacggact agttcattac tacgtagaat cgagaccgag gagagggtta gggataggct
      181 taccgatatc aaccactttg tacaagaaag ttgggtacat ctgggtaaca tacttgtggc
      241 tagtcagatg ttcggcagat attatgggta tctggttggc tgtggctgtt gcagcgaagg
      301 gaacacttgt ggcagatgtt gttgctgcgg acacagtggc tggcgtagca ccgtgcacca
      361 tgggaacaac acttgtagcg ggtgtcatgc acaatattga gcctggtggg agaaatgctg
      421 tatgctgttg taactgcatg ttggctagag cctgttggta ttggaaaata ccagtgttaa
      481 agactgcggt ggcaccgttg gttttttcaa gagcaggcct ctttggtaat gggggaagta
      541 cagcttgagg aattcccatg gcagctgcgg tggctgcagc ctgtgcagct gcagcctggt
      601 tgacctggta ttgggcagcc ttgatcttgg cttgcaaatg tgcaggggga tgaaagtatt
      661 tgcacttttc ccgagagcat ctccctttga tgtaatccat acacacagtg actgtgttgt
      721 cattggtgtc aatcattgtg ct
//
