LOCUS       sequence-217527-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pLX317-SNAI1
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(143..184)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    192..216
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctttccaaa ttttgtaatc cagaggttga ttgtcgactt aacgcgttcg aacagtatga
       61 gaactactca gtcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa
      121 gttacggact agttcattac tacgtagaat cgagaccgag gagagggtta gggataggct
      181 taccgatatc aaccactttg tacaagaaag ttgggcagcg gggacatcct gagcagccgg
      241 actcttggtg cttgtggagc agggacattc gggagaaggt ccgagcacac gcctggcact
      301 ggtacttctt gacatctgag tgggtctgga ggtgggcccg caggttggag cggtcagcga
      361 aggcacggct gcagtgggga caggagaagg gcttctcgcc agtgtgggtc cggacatggc
      421 cttgtagcag ccagggccta gagaaggcct tcccgcaggt tccgcagacg cagggcagcg
      481 tgtggcttcg gatgtgcatc ttgagggcac ccaggctgag gtattccttg ttgcagtatt
      541 tgcagttgaa ggcctttcga gcctggagat ccttggcctc agagagctgg gccagctgct
      601 tgggcacttg gcccaagcct gggaaggcag cataggcctc ggcctccaag gaagagactg
      661 aagtagagga gaaggacgaa ggagccggtg agggtgggct ggggggctgg gagcctttcc
      721 cactgtcctc atctgaca
//
