LOCUS       sequence-21754-e         617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pBabe-Neo-Myr-Flag-AKT1
FEATURES             Location/Qualifiers
     source          1..617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..85
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtcttcaaa tctaaaccaa aggaccccag ccagcgccgg cgcaggatcc gaggttacct
       61 tgactacaaa gacgatgacg acaagcaatt gacaagtttg tacaaaaaag ttggcatgag
      121 cgacgtggct attgtgaagg agggttggct gcacaaacga ggggagtaca tcaagacctg
      181 gcggccacgc tacttcctcc tcaagaatga tggcaccttc attggctaca aggagcggcc
      241 gcaggatgtg gaccaacgtg aggctcccct caacaacttc tctgtggcgc agtgccagct
      301 gatgaagacg gagcggcccc ggcccaacac cttcatcatc cgctgcctgc agtggaccac
      361 tgtcatcgaa cgcaccttcc atgtggagac tcctgaggag cgggaggagt ggacaaccgc
      421 catccagact gtggctgacg gcctcaagaa gcaggaggaa gaagagatgg acttccggtc
      481 gggctcaccc agtgacaact caggggctga agagatggag gtgtccctgg ccaagcccaa
      541 gcaccgcgtg accatgaacg aatttgagta cctgaagctg ctgggcaagg gcactttccg
      601 caaggtgatc ctggtga
//
