LOCUS       sequence-217562-       10381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted DNA methylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Lu Z, Liu Z, Mao W, Wang X, Zheng X, Chen S, Cao B, Huang S, Zhang 
            X, Zhou T, Zhang Y, Huang X, Sun Q, Li JD
  TITLE     Locus-specific DNA methylation of Mecp2 promoter leads to 
            autism-like phenotypes in mice.
  JOURNAL   Cell Death Dis. 2020 Feb 3;11(2):85. doi: 10.1038/s41419-020-2290-x.
  PUBMED    32015323
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2020-02-3"; volume: "11(2):85. doi"; pages: " 
            10.1038/s41419-020-2290-x"
COMMENT     Sequence Label: pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas...
FEATURES             Location/Qualifiers
     source          1..10381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          231..1173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1231..1250
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1231..1249
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1278..1298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1953..1970
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6987..7007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7038..7094
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7095..7805
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTRGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     7508..7527
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7825..7842)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7831..8055
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8217..8234)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8388..8976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8468..8487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9147..9938,9939..10007))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9770..9789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10008..10112)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10187..10206)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(10381,1..1181)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt ggagctctct ggctaactag
     1201 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
     1261 tggctagcac catgggacct aagaaaaaga ggaaggtggc ggccgctggc ggcagcatgt
     1321 tcgaaaccgt gcctgtgtgg aggagacagc cagtccgggt gctgtccctt tttgaagaca
     1381 tcaagaaaga gctgacgagt ttgggctttt tggaaagtgg ttctgacccg ggacaactga
     1441 agcatgtggt tgatgtcaca gacacagtga ggaaggatgt ggaggagtgg ggacccttcg
     1501 atcttgtgta cggcgccaca cctcccctgg gccacacctg tgaccgtcct cccagctggt
     1561 acctgttcca gttccaccgg ctcctgcagt acgcacggcc caagccaggc agccccaggc
     1621 ccttcttctg gatgttcgtg gacaatctgg tgctgaacaa ggaagacctg gacgtcgcat
     1681 ctcgcttcct ggagatggag ccagtcacca tcccagatgt ccacggcgga tccttgcaga
     1741 atgctgtccg cgtgtggagc aacatcccag ccataaggag cagcaggcac tgggctctgg
     1801 tttcggaaga agaattgtcc ctgctggccc agaacaagca gagctcgaag ctcgcggcca
     1861 agtggcccac caagctggtg aagaactgct ttctccccct aagagaatat ttcaagtatt
     1921 tttcaagcag cgggcgcagc ttcagcagcg gactagtgcc ccgcggcagc cacccagctg
     1981 agaagaggaa gcccatccgg gtgctgtctc tctttgatgg aatcgctaca gggctcctgg
     2041 tgctgaagga cttgggcatt caggtggacc gctacattgc ctcggaggtg tgtgaggact
     2101 ccatcacggt gggcatggtg cggcaccagg ggaagatcat gtacgtcggg gacgtccgca
     2161 gcgtcacaca gaagcatatc caggagtggg gcccattcga tctggtgatt gggggcagtc
     2221 cctgcaatga cctctccatc gtcaaccctg ctcgcaaggg cctctacgag ggcactggcc
     2281 ggctcttctt tgagttctac cgcctcctgc atgatgcgcg gcccaaggag ggagatgatc
     2341 gccccttctt ctggctcttt gagaatgtgg tggccatggg cgttagtgac aagagggaca
     2401 tctcgcgatt tctcgagtcc aaccctgtga tgattgatgc caaagaagtg tcagctgcac
     2461 acagggcccg ctacttctgg ggtaaccttc ccggtatgaa caggccgttg gcatccactg
     2521 tgaatgataa gctggagctg caggagtgtc tggagcatgg caggatagcc aagttcagca
     2581 aagtgaggac cattactacg aggtcaaact ccataaagca gggcaaagac cagcattttc
     2641 ctgtcttcat gaatgagaaa gaggacatct tatggtgcac tgaaatggaa agggtatttg
     2701 gtttcccagt ccactatact gacgtctcca acatgagccg cttggcgagg cagagactgc
     2761 tgggccggtc atggagcgtg ccagtcatcc gccacctctt cgctccgctg aaggagtatt
     2821 ttgcgtgtgt gagcggcagc gagacccccg gtaccagcga gagcgccacc cccgagagcg
     2881 acaagaaata ctctattgga ctggctatcg ggacaaactc cgttggctgg gccgtcataa
     2941 ccgacgagta taaggtgcca agcaagaaat tcaaggtgct gggtaatact gaccgccatt
     3001 caatcaagaa gaacctgatc ggagcactcc tcttcgactc cggtgaaacc gctgaagcta
     3061 ctcggctgaa gcggaccgca aggcggagat acacccgccg caagaatcgg atatgttatc
     3121 tgcaagagat ctttagcaac gaaatggcta aggtggacga ctccttcttt caccgcctgg
     3181 aagagagctt tctggtggag gaggataaga aacacgagag gcaccctata ttcggaaata
     3241 tcgtggatga ggtggcttac catgaaaagt atcctacaat ctaccatctg aggaagaagc
     3301 tggtggacag caccgataaa gcagacctga ggctcatcta tctggccctg gctcatatga
     3361 taaagtttag aggacacttt ctgatcgagg gcgacctgaa tcccgataat tccgatgtgg
     3421 ataaactctt cattcaactg gtgcagacat ataaccaact gttcgaggag aatcccataa
     3481 acgcttctgg tgtggatgcc aaggctattc tgtccgctcg gctgtccaag tcacgcagac
     3541 tggagaatct gattgcccaa ctgccaggag aaaagaagaa cggcctgttt gggaacctca
     3601 tcgccctgag cctgggcctg acacctaact tcaagtccaa ttttgatctg gccgaagatg
     3661 ctaaactcca gctctccaag gacacctatg acgatgatct ggacaacctg ctcgcacaga
     3721 taggcgacca gtacgccgat ctctttctgg ctgctaagaa tctctccgac gccattctgc
     3781 tgagcgacat actccgggtc aacactgaga tcaccaaagc acctctgagc gcctccatga
     3841 taaaacgcta tgatgaacac catcaagacc tgactctgct caaagccctc gtgaggcaac
     3901 agctgccaga gaagtacaaa gagatattct tcgaccagag caagaatgga tatgccggat
     3961 acatcgatgg cggagcatca caggaagaat tttacaagtt catcaaacca atcctcgaga
     4021 agatggacgg tactgaagag ctgctggtga agctgaacag ggaggacctg ctgaggaagc
     4081 agaggacctt tgataatggc tccattccac atcagataca cctgggagag ctgcatgcaa
     4141 tcctccgcag gcaggaggat ttctatcctt tcctgaagga taaccgggag aagatagaga
     4201 agatcctgac cttcaggatc ccttattacg tcggccctct ggctagaggc aactcccgct
     4261 tcgcttggat gaccaggaaa tctgaggaga caattactcc ttggaacttc gaagaggtcg
     4321 tggataaggg cgcaagcgcc cagtcattca tcgaacggat gaccaatttc gataagaacc
     4381 tgccaaacga gaaggtcctg cccaaacatt cactcctgta cgagtatttc accgtctata
     4441 acgagctgac taaagtgaag tacgtgaccg agggcatgag gaagcctgcc ttcctgtccg
     4501 gagagcagaa gaaggctatc gttgatctgc tcttcaagac taatagaaag gtgacagtga
     4561 agcagctcaa ggaggattac tttaagaaga tcgaatgctt tgactcagtg gaaatctctg
     4621 gcgtggagga ccgctttaat gccagcctgg gcacttacca tgatctgctg aagataatca
     4681 aagacaaaga tttcctcgat aatgaggaga acgaggacat cctggaagat atcgtgctga
     4741 ccctgactct gttcgaggat agagagatga tcgaagagcg cctgaagacc tatgcccatc
     4801 tgtttgacga taaagtcatg aaacagctca agcggcggcg ctacactggg tggggtagac
     4861 tctccaggaa actcataaac ggcatccgcg acaaacagag cggaaagacc atcctggatt
     4921 tcctgaaatc cgacggattc gctaacagga acttcatgca actgattcac gatgactctc
     4981 tgacatttaa agaggacatc cagaaggcac aggtgagcgg tcaaggcgac agcctgcacg
     5041 agcacatcgc caacctcgct ggatcacccg ccataaagaa gggaatactg cagacagtca
     5101 aggtcgtgga cgaactcgtc aaagtgatgg gtcggcacaa gccagagaat atcgttatcg
     5161 aaatggcaag ggagaaccaa accacccaga agggccagaa gaactctcgg gaacggatga
     5221 aaagaatcga agagggaatt aaggagctgg gatctcagat actgaaggag caccctgtgg
     5281 agaatacaca gctccagaac gagaaactct acctgtacta cctccagaac gggcgggaca
     5341 tgtacgttga ccaggaactc gacatcaacc ggctgtccga ttatgacgtg gacgctattg
     5401 ttccacagtc cttcctcaaa gatgactcca ttgacaacaa ggtgctgacc agatccgata
     5461 aggcccgcgg taagtctgac aatgttccat cagaagaggt ggtcaagaag atgaagaatt
     5521 actggcggca gctcctcaac gccaaactga tcacccagcg gaagtttgac aatctgacta
     5581 aggcagaaag aggaggtctg agcgaactcg acaaggccgg ctttattaag aggcaactgg
     5641 tcgaaacacg ccagattacc aaacacgtgg cacaaatcct cgactctagg atgaacacta
     5701 agtacgatga gaacgataag ctgatcaggg aagtgaaagt gataactctg aagagcaagc
     5761 tggtgtctga cttccggaag gactttcaat tctacaaagt tcgcgaaata aacaattacc
     5821 atcatgctca cgatgcctat ctcaatgctg tcgttggcac cgccctgatc aagaaatacc
     5881 ctaaactgga gtctgagttc gtgtacggtg actataaagt ctacgatgtg aggaagatga
     5941 tagcaaagtc tgagcaagag attggcaaag ccaccgccaa gtacttcttc tactctaata
     6001 tcatgaattt ctttaagact gagataaccc tggctaacgg cgaaatccgg aagcgcccac
     6061 tgatcgaaac aaacggagaa acaggagaaa tcgtgtggga taaaggcagg gacttcgcaa
     6121 ctgtgcggaa ggtgctgtcc atgccacaag tcaatatcgt gaagaagacc gaagtgcaga
     6181 ccggcggatt ctcaaaggag agcatcctgc caaagcggaa ctctgacaag ctgatcgcca
     6241 ggaagaaaga ttgggaccca aagaagtatg gcggtttcga ttcccctaca gtggcttatt
     6301 ccgttctggt cgtggcaaaa gtggagaaag gcaagtccaa gaaactcaag tctgttaagg
     6361 agctgctcgg aattactatt atggagagat ccagcttcga gaagaatcca atcgatttcc
     6421 tggaagctaa gggctataaa gaagtgaaga aagatctcat catcaaactg cccaagtact
     6481 ctctctttga gctggagaat ggtaggaagc ggatgctggc ctccgccgga gagctgcaga
     6541 aaggaaacga gctggctctg ccctccaaat acgtgaactt cctgtatctg gcctcccact
     6601 acgagaaact caaaggtagc cctgaagaca atgagcagaa gcaactcttt gttgagcaac
     6661 ataaacacta cctggacgaa atcattgaac agattagcga gttcagcaag cgggttattc
     6721 tggccgatgc aaacctcgat aaagtgctga gcgcatataa taagcacagg gacaagccaa
     6781 ttcgcgaaca agcagagaat attatccacc tctttactct gactaatctg ggcgctcctg
     6841 ctgccttcaa gtatttcgat acaactattg acaggaagcg gtacacctct accaaagaag
     6901 ttctcgatgc caccctgata caccagtcaa ttaccggact gtacgagact cgcatcgacc
     6961 tgtctcagct cggcggcgac ggttctccca agaagaagag gaaagtctcg agcggtggag
     7021 ctgcaggagg atccggcgcc acgaacttct ctctgttaaa gcaagcagga gacgtggaag
     7081 aaaaccccgg tcctatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     7141 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     7201 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaggg
     7261 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     7321 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     7381 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     7441 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     7501 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     7561 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     7621 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     7681 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     7741 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     7801 agtaagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7861 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7921 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7981 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     8041 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     8101 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8161 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8221 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8281 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8341 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8401 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8461 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8521 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8581 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8641 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8701 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8761 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8821 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8881 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8941 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9001 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9061 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10141 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
    10201 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
    10261 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
    10321 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtgtga
    10381 g
//
