LOCUS       sequence-217600-        9602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P element vector for transposon insertion of Myc-tagged png into 
            Drosophila genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Fenger DD, Carminati JL, Burney-Sigman DL, Kashevsky H, Dines JL, 
            Elfring LK, Orr-Weaver TL
  TITLE     PAN GU: a protein kinase that inhibits S phase and promotes mitosis 
            in early Drosophila development.
  JOURNAL   Development. 2000 Nov;127(22):4763-74.
  PUBMED    11044392
REFERENCE   2  (bases 1 to 9602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2000-11"; volume: "127(22)"; pages: "4763-74"
COMMENT     Sequence Label: C2 M2 png-myc in Casper4
FEATURES             Location/Qualifiers
     source          1..9602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1793..1811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1849..1871)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1971..1990
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2528..2558,2559..2760)
                     /label=P element 3' end
                     /note="P element 3' end"
     CDS             4242..4271
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4278..4307
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4314..4343
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4614..4630)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4615..4631)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4662..4680)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5132..5203,5605..5878,5953..6607,6669..6984,7188..7319,
                     7390..8004)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8783..9337,9338..9368)
                     /label=P element 5' end
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1801 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     1861 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     1921 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     1981 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcaccgaa tcgcgcggaa
     2041 ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg
     2101 ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc
     2161 gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt agcgataaac
     2221 gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac aaatccaaag
     2281 tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat atcttgtttt
     2341 tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt aaacacgatg
     2401 gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg ctactccttg
     2461 cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac gatgagaatg
     2521 gccagaccat gatgaaataa cataaggtgg tcccgtcggc aagagacatc cacttaacgt
     2581 atgcttgcaa taagtgcgag tgaaaggaat agtattctga gtgtcgtatt gagtctgagt
     2641 gagacagcga tatgattgtt gattaaccct tagcatgtcc gtggggtttg aattaactca
     2701 taatattaat tagacgaaat tatttttaaa gttttatttt taataatttg cgagtacgca
     2761 aagcttggct gcaggtcgac ctcgaggcct cgagttaacg ttacgttaac gttaacgttc
     2821 gaggtcgact ctagaggatc tcacttattt ggtacacata gtctctcgaa ttgtttaagt
     2881 atcccatcct gtgaaaatat ctcaaaaatt tacaaatttg tccaaaaagc taagtttatt
     2941 atataaagca attaacccat aagtctcgag tcgaaggccc ccgtagctag tactcattaa
     3001 atttaattag gtttagtatt gtatactata tttaattttg ttaaataaaa taaataaaaa
     3061 tttaggatag tcatactttt cacatggatg gactttttga atggggttag ttcacgatgc
     3121 gttagtttga ttaattaacc agactctaat aacttacttt cattgagcac tgttgctgct
     3181 ggccagatga gcaattttca tatggggttt ccgaagttcc gagttttcgg ctttttccga
     3241 tggaactgtg catctcttgt ttcgaagcaa tcgatagatg gcgcgcttgc gcttttgctg
     3301 gctaactgta catcaaggcg ggatcgcgga ttacgctttc ggaactctgc gcactcgaga
     3361 tggaactggg tgactctaag ctgcgtgcgg taagggtgct gggccagggc acctttggcc
     3421 gggtcttcct ctgccaccaa aacgaggtgc gcggacagcc ggagaggaag gtgtgcgtga
     3481 agcggatcat cgtccggaat ccgaagacag agttgggatt gatcaaggag gaggtgtaca
     3541 tcatctcgca gctgcgccac ccgcacatcg ttgagttcct gcgctccttt agccacgcgg
     3601 gcactgtgaa catcgtgatg gagtacgtgc ccaacggcac cttgagggat gtcatccagc
     3661 agctgccgtc aggcaccggg ggagtcaatc aggagcggct gatgggctac ttccgcgaca
     3721 tggtagtggg actcgagtac ctgcacatcc gctgcgtcat ccaccgagac atcaagcccg
     3781 agaacatgct cctcgacgcc aacgaccgcg tcaagatcgc cgactttggg atcgcgaacg
     3841 ttcatgcgcc cagcactcaa ctgcaggcgg gcatgggcac gcccatgtac atggccccgg
     3901 aggcgatgtc cagccagggc aaggtggatt tcaagtcgga cgtgtggtca ctggggctag
     3961 tcctctacga gctgtgcctc ggacgcagcc ccttcgccgc gtttctcgac aagaacgcca
     4021 cccctgccct actgcacacc gtagtccagg cgctggtccg cccccggctc gactgccagc
     4081 tcatccggcg tctctacgat cccgtgtggg cgcaagtttg cgagctgatg gtcgtttacg
     4141 agcaggagcg ccgcatctgc ctaccagaca tcttccacct cgacgccggc atgactgtga
     4201 ccctctacaa gcattacttc agctacagct actctagagg tgaacaaaag ttgatttctg
     4261 aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac ggtgaacaaa
     4321 aattaatctc agaagaagac ttgaacggat cctaacggaa gccaatggtc agctcaccag
     4381 cacgaggaga cctcaacaaa tatttcattg tacttaagaa ctaggctagc ttaagcgata
     4441 aattaaggtg tgtgctgtgc tttgtgtgcg cctgtgcttt tgccagggga tctgtcagct
     4501 ataaacagta tataatgtat attccaaaac agtctcgctc aatgtcatta acgtagtctt
     4561 taatttacat ttgttcacct attgtacagt aacattaatc ctctaaagct tatcgatacc
     4621 gtcgacctcg agggggggcc cggtaccgaa ttctagtatg tatgtaagtt aataaaaccc
     4681 atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg gatatcattg
     4741 aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt accaggttct
     4801 ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc
     4861 tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc tttcgccacc
     4921 gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg cattttgtca gcggtttcgt
     4981 gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct
     5041 ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg
     5101 taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat
     5161 tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc
     5221 atacatctta agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg
     5281 cgtgagaact gcgacccaca aaaatcccaa accgcaattg cacaaacaaa tagtgacacg
     5341 aaacagatta ttctggtagc tgttctcgct atataagaca atttttgaga tcatatcatg
     5401 atcaagacat ctaaaggcat tcattttcga ctatattctt ttttacaaaa aatataacaa
     5461 ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc
     5521 gtaggatact tcggggtact ttttgttcgg ggttagatga gcataacgct tgtagttgat
     5581 atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag ctgcattaac
     5641 cagggcttcg ggcaggccaa aaactacggc acgctccggc cacccagtcc gccggaggac
     5701 tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca caatatggac
     5761 atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa ccggacacgc
     5821 ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct caagaacggt
     5881 gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc caattaccaa
     5941 tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg atgggcagtt
     6001 ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc
     6061 aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc aaggagatgc
     6121 aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta acggccaggg
     6181 aacacctgat tttccaagcc atggtgcgga tgccacgaca tctgacctat cggcagcgag
     6241 tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag cacacgatca
     6301 tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg
     6361 cctccgaggc tctaaccgat ccgccgcttc tgatctgcga tgagcccacc tccggactgg
     6421 actcctttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga
     6481 ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt gacaagatcc
     6541 ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc gtcgacttct
     6601 tttcctagtg agttcgatgt gtttattaag ggtatctagt attacataac atctcaactc
     6661 ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt ttacgtacag
     6721 gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc caagatatgc
     6781 gacaattttg ccattagcaa agtagcccgg gatatggagc agttgttggc caccaaaaat
     6841 ctggagaagc cactggagca gccggagaat gggtacacct acaaggccac ctggttcatg
     6901 cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta
     6961 aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg atataacgct
     7021 tacaattctt ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt ccacaccctt
     7081 cttagttttt ttcaatgaga tgtatagttt atagttttgc agaagataaa taaatttcat
     7141 ttaactcgcg aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct
     7201 tgattggcct catctttttg ggccaacaac tcacgcaagt gggtgtgatg aatatcaacg
     7261 gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg
     7321 taagtcatgt ttagaataca tttgcatttc aataatttac taactttcta atgaatcgat
     7381 tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt
     7441 tatcgctgtg acacatactt tctgggcaaa acgattgccg aattgccgct ttttctcaca
     7501 gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg
     7561 cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga
     7621 tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt
     7681 atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac
     7741 ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac
     7801 caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc
     7861 agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac
     7921 tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga
     7981 cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt
     8041 tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta
     8101 tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca
     8161 tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct
     8221 tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt atatacatac
     8281 ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct
     8341 ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga
     8401 gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc
     8461 gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt
     8521 gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa
     8581 ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt
     8641 gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat
     8701 atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat gcttggattt
     8761 cactggaact agaattaatt cggctgctgc tctaaacgac gcatttcgta ctccaaagta
     8821 cgaatttttt ccctcaagct cttattttca ttaaacaatg aacaggacct aacgcacagt
     8881 cacgttattg tttacataaa tgattttttt tactattcaa acttactctg tttgtgtact
     8941 cccactggta tagccttctt ttatcttttc tggttcaggc tctatcactt tactaggtac
     9001 ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct tttgcaggtg cagccttcca
     9061 ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt tcaccaaggc tgcacccaag
     9121 gctctgctcc cacaattttc tcttaatagc acacttcggc acgtgaatta attttactcc
     9181 agtcacagct ttgcagcaaa atttgcaata tttcattttt ttttattcca cgtaagggtt
     9241 aatgttttca aaaaaaaatt cgtccgcaca caacctttcc tctcaacaag caaacgtgca
     9301 ctgaatttaa gtgtatactt cggtaagctt cggctatcga cgggaccacc ttatgttatt
     9361 tcatcatggg ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc
     9421 tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg
     9481 cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat
     9541 gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaaggcg
     9601 tt
//
