LOCUS       sequence-217750-       10217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P element vector for transposon insertion of png-GFP into Drosophila
            genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10217)
  TITLE     Orr-Weaver plasmids
REFERENCE   2  (bases 1 to 10217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C2 G10 png/GFP in casper4
FEATURES             Location/Qualifiers
     source          1..10217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(824..854,855..1409)
                     /label=P element 5' end
     CDS             complement(join(2188..2802,2873..3004,3208..3523,3585..4239,
                     4314..4587,4989..5060))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     5512..5530
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     5561..5577
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5562..5578
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(5855..6565,6566..6568,6569..6571))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5855..6565,6566..6568,6569..6571))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5897..5918)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6246..6265
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6505..6526
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(8047..8248,8249..8279)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(8817..8836)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8936..8958
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8996..9014)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9082..9186
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9187..9255,9256..10047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9405..9424)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccttctt
      601 cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg
      661 ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag
      721 ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa
      781 cttctccgcc gccgatctgc cgctggacta cgtgggtctg gcccatgatg aaataacata
      841 aggtggtccc gtcgatagcc gaagcttacc gaagtataca cttaaattca gtgcacgttt
      901 gcttgttgag aggaaaggtt gtgtgcggac gaattttttt ttgaaaacat taacccttac
      961 gtggaataaa aaaaaatgaa atattgcaaa ttttgctgca aagctgtgac tggagtaaaa
     1021 ttaattcacg tgccgaagtg tgctattaag agaaaattgt gggagcagag ccttgggtgc
     1081 agccttggtg aaaactccca aatttgtgat acccacttta atgattcgca gtggaaggct
     1141 gcacctgcaa aaggtcagac atttaaaagg aggcgactca acgcagatgc cgtacctagt
     1201 aaagtgatag agcctgaacc agaaaagata aaagaaggct ataccagtgg gagtacacaa
     1261 acagagtaag tttgaatagt aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt
     1321 taggtcctgt tcattgttta atgaaaataa gagcttgagg gaaaaaattc gtactttgga
     1381 gtacgaaatg cgtcgtttag agcagcagcc gaattaattc tagttccagt gaaatccaag
     1441 cattttctaa attaaatgta ttcttattat tatagttgtt atttttgata tatataaaca
     1501 acactattat gcccaccatt tttttgagat gcatctacac aaggaacaaa cactggatgt
     1561 cactttcagt tcaaattgta acgctaatca ctccgaacag gtcacaaaaa attaccttaa
     1621 aaagtcataa tattaaatta gaataaatat agctgtgagg gaaatatata caaatatatt
     1681 ggagcaaata aattgtacat acaaatattt attactaatt tctattgaga cgaaatgaac
     1741 cactcggaac catttgagcg aaccgaatcg cgcggaacta acgacagtcg ctccaaggtc
     1801 gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga gacagcgaaa gagcaactac
     1861 gaaacgtggt gtggtggagg tgaattatga agagggcgcg cgatttgaaa agtatgtata
     1921 taaaaaatat atcccggtgt tttatgtagc gataaacgag tttttgatgt aaggtatgca
     1981 ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct acttgtgggg atgttcgaag
     2041 gggaaatact tgtattctat aggtcatatc ttgtttttat tggcacaaat ataattacat
     2101 tagctttttg agggggcaat aaacagtaaa cacgatggta ataatggtaa aaaaaaaaaa
     2161 caagcagtta tttcggatat atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc
     2221 cagatatgcg agcacccgga agctcacgat gagaatggcc agacccacgt agtccagcgg
     2281 cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt
     2341 ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag
     2401 cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg
     2461 caccgagccc gagttcaaga agaagccgcc aaagagcagg aatggtatga taaccggcgg
     2521 acccacagac agcgccatcg aggtcgagga gctggcgcag gatattagat atccgaagga
     2581 cgttgacaca ttggccacca gagtgaccag cgccaggcag ttgaagaagt gcagcactcc
     2641 ggcccgcagt ccgatcatcg gataggcaat cgccgtgaag accagtggca ctgtgagaaa
     2701 aagcggcaat tcggcaatcg ttttgcccag aaagtatgtg tcacagcgat aaagtcgact
     2761 tcgggcctcc ctcataaaaa ctggcagctc tgaggtgaac acctaaatcg aatcgattca
     2821 ttagaaagtt agtaaattat tgaaatgcaa atgtattcta aacatgactt acatttatcg
     2881 tggcaaagac gttttgaaag gtcatgttgg tcaggaagag gaagatggct ccgttgatat
     2941 tcatcacacc cacttgcgtg agttgttggc ccaaaaagat gaggccaatc aagatggcaa
     3001 ccatctgcaa attaaaatgt tactcgcatc tcattaatat tcgcgagtta aatgaaattt
     3061 atttatcttc tgcaaaacta taaactatac atctcattga aaaaaactaa gaagggtgtg
     3121 gaatcaggca attctatcta aaatctagcg aatttgtttc caagaattgt aagcgttata
     3181 tcatttgttt ccactggaac cactcaccgt tgtctgaata agtcgcactt ttacgaggag
     3241 tggttccttg agcaccgaca gccaggatcg ccacaggacc gcccggaact gcatgaacca
     3301 ggtggccttg taggtgtacc cattctccgg ctgctccagt ggcttctcca gatttttggt
     3361 ggccaacaac tgctccatat cccgggctac tttgctaatg gcaaaattgt cgcatatctt
     3421 ggcgatccga tcacgggact cgatctcccg tccgggcaca acggccaaca cctgtacgta
     3481 aaagtccgcc ggattgtagt tggtaggaca ctgggcaccc acgctggata ggagttgaga
     3541 tgttatgtaa tactagatac ccttaataaa cacatcgaac tcactaggaa aagaagtcga
     3601 cggcttcgct gggagtgccc aagaaagcta ccctgccctc ggccatcaga aggatcttgt
     3661 caaagagctc aaacagctcg gaagacggct gatgaatggt caggatgacg gtcttgccct
     3721 tctgcgacag cttcttcagc acctggacga cgctgtgggc ggtaaaggag tccagtccgg
     3781 aggtgggctc atcgcagatc agaagcggcg gatcggttag agcctcggag gcgaatgcca
     3841 gacgcttcct ttctccgccg gacagacctt tcaccctgcc gggcacaccg atgatcgtgt
     3901 gctgacattt gctgagcgaa agctcctgga tcacctgatc cacgcgggcc actcgctgcc
     3961 gataggtcag atgtcgtggc atccgcacca tggcttggaa aatcaggtgt tccctggccg
     4021 ttagggagcc gataaagagg tcatcctgct ggacataggc gcacctggcc tgcatctcct
     4081 tggcgtccac aggttggcca ttgagcagtc gcatcccgga tggcgatact tggatgccct
     4141 gcggcgatcg aaaggcaagg gcattcagca gggtcgtctt tccggcaccg gaactgccca
     4201 tcacggccaa aagttcgccc ggataggcca cgccgcaaac tgagtttcaa attggtaatt
     4261 ggacccttta ttaagatttc acacagatca gccgactgcg aatagaaact caccgttctt
     4321 gagcaaatgt ttcctgggcg ccggtatgtg tcgctcgttg cagaatagtc cgcgtgtccg
     4381 gttgaccagc tgccgccatc cggagcccgg ctgattgacc gccccaaaga tgtccatatt
     4441 gtgccaggca taggtgaggt tctcggctag ttggccgctc cctgaaccgg agtcctccgg
     4501 cggactgggt ggccggagcg tgccgtagtt tttggcctgc ccgaagccct ggttaatgca
     4561 gctctgcgaa gccgctccgc tgtcaccctg caatgatagg ggatctcaaa tatcaactac
     4621 aagcgttatg ctcatctaac cccgaacaaa aagtaccccg aagtatccta cgaagtaggt
     4681 ttatactttt atttattttt tgtgcatcta ggatcagctt aaaatatctg gttgttatat
     4741 tttttgtaaa aaagaatata gtcgaaaatg aatgccttta gatgtcttga tcatgatatg
     4801 atctcaaaaa ttgtcttata tagcgagaac agctaccaga ataatctgtt tcgtgtcact
     4861 atttgtttgt gcaattgcgg tttgggattt ttgtgggtcg cagttctcac gccgcagaca
     4921 atttgatgtt gcaatcgcag ttcctataga tcaagtgaac ttaagatgta tgcacatgta
     4981 ctactcacat tgttcagatg ctcggcagat gggtgtttgc tgcctccgcg aattaatagc
     5041 tcctgatcct cttggcccat tgccgggatt tttcacactt tcccctgctt acccacccaa
     5101 aaccaatcac caccccaatc actcaaaaaa caaacaaaaa taagaagcga gaggagtttt
     5161 ggcacagcac tttgtgttta attgatggcg taaaccgctt ggagcttcgt cacgaaaccg
     5221 ctgacaaaat gcaactgaag gcggacattg acgctacgta acgctacaaa cggtggcgaa
     5281 agagatagcg gacgcagcgg cgaaagagac ggcgatattt ctgtggacag agaaggaggc
     5341 aaacagcgct gactttgagt ggaatgtcat tttgagtgag aggtaatcga aagaacctgg
     5401 tacatcaaat acccttggat cgaagtaaat ttaaaactga tcagataagt tcaatgatat
     5461 ccagtgcagt aaaaaaaaaa aatgtttttt ttatctactt tccgcaaaaa tgggttttat
     5521 taacttacat acatactaga attcggtacc gggccccccc tcgaggtcga cggtatcgat
     5581 aagctttaga ggattaatgt tactgtacaa taggtgaaca aatgtaaatt aaagactacg
     5641 ttaatgacat tgagcgagac tgttttggaa tatacattat atactgttta tagctgacag
     5701 atcccctggc aaaagcacag gcgcacacaa agcacagcac acaccttaat ttatcgctta
     5761 agctagccta gttcttaagt acaatgaaat atttgttgag gtctcctcgt gctggtgagc
     5821 tgaccattgg cttccgttag gatctgagtc cggacttgta cagctcgtcc atgccgagag
     5881 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     5941 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     6001 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
     6061 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
     6121 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     6181 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     6241 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     6301 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
     6361 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     6421 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     6481 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     6541 cggtgaacag ctcctcgccc ttgctcacca tggagtagct gtagctgaag taatgcttgt
     6601 agagggtcac agtcatgccg gcgtcgaggt ggaagatgtc tggtaggcag atgcggcgct
     6661 cctgctcgta aacgaccatc agctcgcaaa cttgcgccca cacgggatcg tagagacgcc
     6721 ggatgagctg gcagtcgagc cgggggcgga ccagcgcctg gactacggtg tgcagtaggg
     6781 caggggtggc gttcttgtcg agaaacgcgg cgaaggggct gcgtccgagg cacagctcgt
     6841 agaggactag ccccagtgac cacacgtccg acttgaaatc caccttgccc tggctggaca
     6901 tcgcctccgg ggccatgtac atgggcgtgc ccatgcccgc ctgcagttga gtgctgggcg
     6961 catgaacgtt cgcgatccca aagtcggcga tcttgacgcg gtcgttggcg tcgaggagca
     7021 tgttctcggg cttgatgtct cggtggatga cgcagcggat gtgcaggtac tcgagtccca
     7081 ctaccatgtc gcggaagtag cccatcagcc gctcctgatt gactcccccg gtgcctgacg
     7141 gcagctgctg gatgacatcc ctcaaggtgc cgttgggcac gtactccatc acgatgttca
     7201 cagtgcccgc gtggctaaag gagcgcagga actcaacgat gtgcgggtgg cgcagctgcg
     7261 agatgatgta cacctcctcc ttgatcaatc ccaactctgt cttcggattc cggacgatga
     7321 tccgcttcac gcacaccttc ctctccggct gtccgcgcac ctcgttttgg tggcagagga
     7381 agacccggcc aaaggtgccc tggcccagca cccttaccgc acgcagctta gagtcaccca
     7441 gttccatctc gagtgcgcag agttccgaaa gcgtaatccg cgatcccgcc ttgatgtaca
     7501 gttagccagc aaaagcgcaa gcgcgccatc tatcgattgc ttcgaaacaa gagatgcaca
     7561 gttccatcgg aaaaagccga aaactcggaa cttcggaaac cccatatgaa aattgctcat
     7621 ctggccagca gcaacagtgc tcaatgaaag taagttatta gagtctggtt aattaatcaa
     7681 actaacgcat cgtgaactaa ccccattcaa aaagtccatc catgtgaaaa gtatgactat
     7741 cctaaatttt tatttatttt atttaacaaa attaaatata gtatacaata ctaaacctaa
     7801 ttaaatttaa tgagtactag ctacgggggc cttcgactcg agacttatgg gttaattgct
     7861 ttatataata aacttagctt tttggacaaa tttgtaaatt tttgagatat tttcacagga
     7921 tgggatactt aaacaattcg agagactatg tgtaccaaat aagtgagatc ctctagagtc
     7981 gacctcgaac gttaacgtta acgtaacgtt aactcgaggc ctcgaggtcg acctgcagcc
     8041 aagctttgcg tactcgcaaa ttattaaaaa taaaacttta aaaataattt cgtctaatta
     8101 atattatgag ttaattcaaa ccccacggac atgctaaggg ttaatcaaca atcatatcgc
     8161 tgtctcactc agactcaata cgacactcag aatactattc ctttcactcg cacttattgc
     8221 aagcatacgt taagtggatg tctcttgccg acgggaccac cttatgttat ttcatcatgg
     8281 tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc
     8341 ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt tttttaccat
     8401 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
     8461 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
     8521 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
     8581 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
     8641 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
     8701 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
     8761 cgttagttcc gcgcgattcg gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     8821 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     8881 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     8941 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     9001 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     9061 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     9121 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     9181 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9241 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9301 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9361 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9421 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9481 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9541 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9601 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9661 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9721 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9781 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9841 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9901 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9961 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10021 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    10081 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10141 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10201 aagatcaaag gatcttc
//
