LOCUS       sequence-217876-       15357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15357)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 15357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU36.2-2.2
FEATURES             Location/Qualifiers
     source          1..15357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1521..1820
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        5659..5879
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5880..6683
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             8410..8421
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(11504..11524)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11504..11522)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(11861..12449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11941..11960)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12620..13411,13412..13480))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13243..13262
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13481..13585)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13612..14954
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(14575..14622)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     14995..15013
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15332..15348)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15332..15348)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggatactt ttactatgaa aaaggacaaa ggtggttaca
      541 ggcagattaa taaatcgttg aatatctgtg ccttcgatga gtaccttcgg ggccaaactg
      601 cgcgactacc gcatatagag agtgttgaac agctcactcc gagagtactt cgcattctcg
      661 gtcagaaccc gggaaagttc acgcatcaag gaactaatac atacatcgtc ggcaccggcc
      721 aacgccgact cattatcgac acaggtggcg gccagcctga atggaccgaa ctaatcgcgt
      781 cgacttttaa atcaatgaac ataagcttat ctcatgttct gttgacccat tggcacggtg
      841 accacactgg tggtgtctca ggtttattgc gcctgtatcc gtatctcacg gcttcgatct
      901 acaagaacga gccgaatcag ggccagcaga acatcgctga cggacagata ttctccgttg
      961 atggcgcaac aatccatgct atccatgcac ccggacattc agaggaccat atgtgtttta
     1021 tcctagaaga ggagaatgcg atgtttaccg gcgataatat ccttggccat ggtactagtg
     1081 ctgtagaaga cctaggcacc ttcatggcca gcctcgagaa gatggccgcc cagaattgtc
     1141 aaatcggtta ttctgctcat ggcgctacaa tcgcaaactt gccagcaaag attgctggag
     1201 aattagaaag caagtggcga cgagagaagc aagttatgaa aggactggcg cgagttcgca
     1261 tccgtggcga gaaaagcgcc accgtcaggg acctcgtaac tgagatctac ggcggttcgc
     1321 tagacaaaga cacgaggacg ctcgcattgg aaccatttat tgatgaagtg ttacggaagt
     1381 tggcggatga ctccaaggtt gcttttgaaa tgagaggagg caagaagaag tggttctctg
     1441 tggaggaggt ggaacaaaca accacctggc ggttcacagc cacgaataaa agttcaccag
     1501 ctgtacctgt tcaagtttga gcggatctct tatgtcttta cgatttatag ttttcattat
     1561 caagtatgcc tatattagta tatagcatct ttagatgaca gtgttcgaag tttcacgaat
     1621 aaaagataat attctacttt ttgctcccac cgcgtttgct agcacgagtg aacaccatcc
     1681 ctcgcctgtg agttgtaccc attcctctaa actgtagaca tggtagcttc agcagtgttc
     1741 gttatgtacg gcatcctcca acaaacagtc ggttatagtt tgtcctgctc ctctgaatcg
     1801 tctccctcga tatttctcat tttccttcgc atgccagcat tgaaatgatc gaagttcaat
     1861 gatgaaacgg taattcttct gtcatttact catctcatct catcaagtta tataattcta
     1921 tacggatgta atttttcact tttcgtcttg acgtccaccc tataatttca attattgaac
     1981 cctcacgatc cagttctcca gtgacacagc ctttatctgg tcaaaccttt ctttctaatc
     2041 acctatgctg atgcttaatt aagggatttt tgtctccatc aacggcatgc gcccaaaaat
     2101 gacgtttttt ttaacccata gacacgaaac tacccatttt ccaccggcct gacctaccac
     2161 cggaacaacg gccatctcca acttgcaagt tggggaaatt aagagcatcg caggtttaat
     2221 ggaagaaaaa aaaaaggtac agcacagcgc aaatggagtt agttccctta tgtcacacac
     2281 tcacacacag tcggtcagat caagcatact gggtgcgtat aaatagagtg gccattgcca
     2341 ccctgtttat ctcaaaatct gtcttgttag tggtcttctc cctttttcag gttacaattc
     2401 tcttgtttct acttagtata taagtatatc aagctatatt aagcatacta tcaactgtca
     2461 actctatcct caaaatacaa tacaaaatgg acgaggtgag atatcgcgac cagctcgccg
     2521 cagccgtcaa gagcctcatc gatagctgtc gaggagctga ttcttccgac caactggcgg
     2581 caagcgctga tactgaagaa aacaaggact atgcttcttt aaacagagcc aggtcaagta
     2641 ttcttgccag tactgcagca atcaagtcac tagttgctga accaaaaaac ttgcttcagg
     2701 atttcgctcg tcagattgag atccttgcca gtcttcggtg gctagcagaa tttcaggttt
     2761 tggcgtgcat cccgtttgac gaaagtttgc ctgtcgagga cctggcagac ttggtaggag
     2821 ttcccgagag ccagctggtc cgggttatcc gtctgacggc cacatgcggc tttttacgag
     2881 agccaatatc gggttttgta tcccacagcg ctctctcggc acaattcata accaatcagt
     2941 ctttacttga cgccactgtg tttattgccg acttggccac tcctacagca ttgcagatgc
     3001 cacaagcaac gcagcgcttt gggaattcct cgagtgccac agatagcgca tacaatttgg
     3061 ctttgaatac ggtgcgaccg tttcatgtgg ctgtccagga gcgctctcgg ctgcgccgcc
     3121 aatggtcggc ctacctttgc cacgcagctg ggttgcatca agaagaagag atagttgacg
     3181 ttctatcgaa gctcaactgg tcaaaccttg gaaatgcatg tatagttgag gtcggcgcaa
     3241 agtcaacttc aatggcgggt tctctttcca aaatgttccc aagcttgcga ttcgtcgtgc
     3301 aaattgacaa cacaagatct tatttgttag atcaggacta cgcgtggcag agtgcagttc
     3361 taggaggctc gacccaagat agctctggct ccgggtctag tccttccccc acaaaccctt
     3421 tcgtcacggt ttcttatcgc gccatgggta tgcctcagtc cgtgacagat gctgccgtat
     3481 atataatcca cattcctgtg gcatcatccg gattgacaat taaagcagag ctggatgatt
     3541 atttgggtgt tcttcgagcc agcggaggta tcatgctcat catgacggca cggttgcttc
     3601 ccgagccagg ctcactctcc aactcagagg ctgagagtat ggcgcgcgca cgggatctga
     3661 gcatgctaca tctagctaac gagggtgaga tggaaatttc cgatctcctg agtactataa
     3721 agacggtggg ggacagcgct gggaaactcg ttgttactaa tcacctacgc tcaaacgacg
     3781 gcataattct agctttaacc atcaagtatc aagcttacat tattcataag cctacattcg
     3841 atagtagatc accagcattt acctccgaca cactcccgat cgttggctcc tggaactatt
     3901 ttacccagca caaaaatttt tggaaggact gcgtcgcaga gtctaagaac ggccacttta
     3961 gtttttggat cggcaagaac catatcattg gcctatctgg cgcggctgct cgcaagatgt
     4021 atctagacag ccgcgatctc gatcctattg gagggtctat gcttattggg catggaccag
     4081 acattataga tggtaaatca tccattatac ctgccatctg gacgactacc gtaaccggcg
     4141 accgtctctt tgcccatcgc cggcttctcg atttgcaaaa atcggaacag ctcacgaagc
     4201 gcctcgctcg tgtgaccaag gactgccgcg acacatttag ttcaatgtct acaaactcgt
     4261 ttggggtcat aaatccaact aagtcgtgct atcccattgt tgtgaagcag tactctcgca
     4321 ttgtgggcgc cgatgaaatc tcgaataacc caaagatgct agaaaagctc caatattaca
     4381 tgcatatcct acagaccact tcaagtgtcc atctccttgc ttttccttgg ctgtcatact
     4441 ttagtgtggc ctattggaaa cgtcgctatg gccgttgggg gttttctaac attgtgacgc
     4501 ctattgtcaa caagcgaatg atgaagggcg cacttcgcgt tgatgatgcg ctgcaacttc
     4561 ttattgataa tggcgacagc aaaacctata ttatcgactt cctcattagc atgttattta
     4621 tcgccggtgc caacgccact gttttaaccg gtgccatgct aaatatcatc gcccaccacc
     4681 cagactggca agagagaata taccgtgaga ttaaggctac cgctgcatcc cactcaacga
     4741 acaaaggtgc atcattggta gaccaactcg actcaatgcc attgtccgcc tgggaatcac
     4801 tttctcagtc tcttgaactt tgtttcaagg aaaccatccg catgtgggct gttttcccag
     4861 taagtcgcag aaatatcacc gatcatatta taaatatcgc aggaacacac gaggttatcc
     4921 cacctggtgg ctatactgca tatcatagca ctgatgtaca ttataacgaa aacctctacc
     4981 cggatccgat gagatgggac ccggaacgct tttgcgatgg tcgcgaagag tttaaaaagg
     5041 aggattatgg atatattggt tggggtcagg gacgtcatcc ttgcattggc atgcgttggg
     5101 ctaaactcca gcaaaatata aatatcgcct atcctctagc tatgtacaag tggagcggct
     5161 gcgatgagaa tggacagccc aaccctaact ttgcacataa gccaacagta aacagaggcg
     5221 cccccactct gccgatggga ctttactgta aatatgttcc gcgaggagat acataaacaa
     5281 atcgctctta aatatatacc taaagaacat taaagctata ttataagcaa agatacgtaa
     5341 attttgctta tattattata cacatatcat atttctatat ttttaagatt tggttatata
     5401 atgtacgtaa tgcaaaggaa ataaatttta tacattattg aacagcgtcc aagtaactac
     5461 attatgtgca ctaatagttt agcgtcgtga agactttatt gtgtcgcgaa aagtaaaaat
     5521 tttaaaaatt agagcacctt gaacttgcga aaaaggttct catcaactgt ttaaaaggag
     5581 gatatcaggt cctatttctg acaaacaata tacaaattta gtttcaaaga tgaatcagtg
     5641 cgcgaaggac ataactcatt caattcaatt catcattttt tttttattct tttttttgat
     5701 ttcggtttct ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga
     5761 aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt
     5821 gcccagtatt cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca
     5881 tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc
     5941 tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca
     6001 ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac
     6061 atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat
     6121 ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag
     6181 tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg
     6241 cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa
     6301 caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta
     6361 ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg
     6421 gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga
     6481 cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg
     6541 atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg
     6601 gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga
     6661 gaagatgcgg ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact
     6721 cacaaattag agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg
     6781 cacagatgcg taaggagaaa ataccgcatc aggataggaa aaaaccgagc ttcctttcat
     6841 ccggcgcggc tgtgttctac atatcactga agctccgggt attttaagtt atacaaggga
     6901 aagatgccgg ctagactagc aagttttagg ctgcttaaca ttatggatag gcggataaag
     6961 ggcccaaaca ggattgtaaa gcttagacgc ttctggttgg acaatggtac gtttgtgtat
     7021 taagtaaggc ttggctgggg atagcaacat tgggcagagt atagaagacc acaaaaaaaa
     7081 ggtatataag ggcagagaag tctttgtaat gtgtgtaact tctcttccat gtgtaatcag
     7141 tatttctact tacttcttaa atatacagaa gtaagacaga taaccaacag cctttcccag
     7201 atatacatat atatctttat ttcagcttaa acaataatta tatttgttta actcaaaaat
     7261 aaaaaaaaaa aaccaaactc acgcaactaa ttattccata ataaaataac aacatgaata
     7321 ccaccaacat tgcccgtggg gcaaatggac acgtcaaaat aagctcgaaa tgggacgaga
     7381 ttacagctgt cgaccgagaa acgtcagaaa agctacgatt ggggcttcta ccctcgtcga
     7441 agaacataga catcaatatt ctaaactctc ctggtattgt atatcctagt gtatgctctt
     7501 cttccgctgt atgtcaagta aagactgtca ctacaccctc tgcagaagac atagcggttt
     7561 cagaagcaat agatgcaatt gaagatctta caagcgactt tgaagctgtc acagatgaga
     7621 gcaataccga ggaagttcca agtgagctga ctccaaaggt caccaaacgg aaaaatacac
     7681 gagtttccaa gctaaaatat gccgcggaac cttctcaaaa gacccaatgg ccccgaacct
     7741 ggttaggtaa cgacagcctg tgcaacgaca agtctagtcc caagaccatc gaatacggct
     7801 gcaagaatag tcccgcatca tctctgtgga cagatagcga atcttgggat actccaagct
     7861 tcctcagata tgatgcagaa aggccctcac ggtatcgatt tgtgcggccg cgcgaaacag
     7921 ttcgccagcg agttacttca ctgaagcata aagttgttga aattgcgaga actgatcttg
     7981 gctgggtgaa agaggccatg ggcaacattc gtaccaaagc tagcccggaa aatcccaacc
     8041 atagagttgg aagtgccgaa aactcacgcg agagttcaag agacgactct acttatttga
     8101 gtctgagggg cggagcgggc gatatttcaa gcttgaacgg agcgggaaat gacatttttg
     8161 ggattggcgg ggggactggt gattgttgtg ctcaactttc tgcagagttg ggatcctctg
     8221 tgggttttga attgagcagc atctatgcca actcgcagag ttcgtattgg gcaaaacaac
     8281 aatcggagct cactccaagc tgtattattg tgccttcgac ggcgtctgaa gtcgcggttg
     8341 ccatctccat catttcaaca atcgagtcgt gcaacttcgc agtcaaaggt gcagggcacg
     8401 gaacagttgt gggagctaca aatatcaatg gaggtgtcaa gtttgatatg agccgcctga
     8461 acgaaattga gaccaactcg gaaggaactg tcaccaggat cggggccgga agccaatggg
     8521 gagaggtcta tgagtatctt gacaacagag gcctttccgt tgctggaggt cgaaacggcg
     8581 atgttggcgt agctggagtg ctccttggag gcggaatatc attctatggt ccccgagtag
     8641 gctgggcaac ggataatatt ttgaatgtcg aagtcgtgct tgcctcggga gagattacca
     8701 acgccaattc cacttcaaac caagacctac ttaaagcatt gaaaggcggg aacagcaact
     8761 tcggtatcgc cacattattt gatctgcgca cattccccca aggtgaaatg tgggtgggtt
     8821 atctcggtca atccattgat agccgcgatg aagtttttaa agccatcgcc gatatcgcgt
     8881 caaatgccaa tgaggaccct ttcgctgctt tggtgggcga cttcaagttt aattccgcca
     8941 ccaaatcttg ggtcatgaac catactgtgg catatacaaa gccagttgcc aacccgccga
     9001 tatttcagcc attggttaac atcaagcccc agttgagcaa caacattgca atcacaaaca
     9061 ttagcaccat tgctacgggg cagaagaatg gagccaatgt tgcttataac aagaaccatc
     9121 tgtcttatac gggcacgtac ggaaatgatg cggcgcttct ctccaagatt ttcgacatca
     9181 gcaacgccac cgttcacaag ataatgtcgc agaccaatgg tgggatcaaa tggacaacaa
     9241 tgatggaacc atttccagcg ctggtggaca gctttggaga taaaaacggc ggaaactcac
     9301 tcggtttgag cgcagagact ggagatcgta ttgtcatctt gcaattggca caatttgagg
     9361 acagagatgc gaacgacatg gtcgacaaag agcttcacaa catgtttgag gagattgcaa
     9421 aagttgcatc ggaaatggga ctcctccgcc gctttcggta cttgaattat gcggacaagc
     9481 agcagaaccc cattgcctca tacgggccgg aaaatgtggc gcaattgaag gctacaagca
     9541 agaaatacga tccgaagggt ttattccaga gacaagcgcc tggtgggttt aagcttggaa
     9601 tgcagcagtg aagtgctttt aactaagaat tattagtctt ttctgcttat tttttcatca
     9661 tagtttagaa cactttatat taacgaatag tttatgaatc tatttaggtt taaaaattga
     9721 tacagtttta taagttactt tttcaaagac tcgtgctgtc tattgcataa tgcactggaa
     9781 ggggaaaaaa aaggtgcaca cgcgtggctt tttcttgaat ttgcagtttg aaaaataact
     9841 acatggatga taagaaaaca tggagtacag tcactttgag aaccttcaat cagctggtaa
     9901 cgtcttcgtt aattggatac tcaaaaaaga tggatagcat gaatcacaag atggaaggaa
     9961 atgcgggcca cgaccacagt gatatgcata tgggagatgg agatgatacc tccattgggc
    10021 cgatgaagtt agtcgacgga tagaagcggt tgtccccttt cccggcgagc cggcagtcgg
    10081 gccgaggttc ggataaattt tgtattgtgt tttgattctg tcatgagtat tacttatgtt
    10141 ctctttaggt aaccccaggt taatcaatca cagtttcata ccggctagta ttcaaattat
    10201 gacttttctt ctgcagtgtc agccttacga cgattatcta tgagctttga atatagtttg
    10261 ccgtgattcg tatctttaat tggataataa aatgcgaagg atcgatgacc cttattatta
    10321 tttttctaca ctggctaccg atttaactca tcttcttgaa agtatataag taacagtaaa
    10381 atataccgta cttctgctaa tgttatttgt cccttatttt tcttttcttg tcttatgcta
    10441 tagtacctaa gaataacgac tattgttttg aactaaacaa agtagtaaaa gcacataaaa
    10501 gaattaagaa aatggctacg ctggatgagt cagggatgcc taagttgtca gagtcgacaa
    10561 aagacggcta tctgtgcctt accgtttgtg ggtaccgtaa accaggcatg aacgaggaag
    10621 attaccgcaa ttacatggtc aatgtcttag cgcctatggc cagggacctt atggtgaagt
    10681 acgatatcaa acgatggacc cagatccaca atcaaagcgc cactcgggat ttaatgaacc
    10741 agctatctga tcatcagatg aacaatattg cagacttcga ctgtttcagc caggtggttt
    10801 tcaagagtat tgaagacttc aagcgtatga aacaagaccc atggtacaag gagcatttag
    10861 gcaaccacgg aagttttgct gatacgaagc gaagcaaggt gacaataggc tggattgaag
    10921 aatttgtgag agatggtgag gtagtcgatg gcttcaagat ttgctaggga gattgataag
    10981 acttttctag ttgcatatct tttatattta aatcttatct attagttaat tttttgtaat
    11041 ttatccttat atatagtctg gttattctaa aatatcattt cagtatctaa aaattcccct
    11101 cttttttcag ttatatctta acaggcgaca gtccaaatgt tgatttatcc cagtccgatt
    11161 catcagggtt gtgaagcatt ttgtcaatgg tcgaaatcac atcagtaata gtgcctctta
    11221 cttgcctcat agaatttctt tctcttaacg tcaccgtttg gtcttttata gtttcgaaat
    11281 ctatggtgat accaaatggt gttcccaatt catcgttacg ggcgtatttt ttaccaattg
    11341 aagtattgga atcgtcaatt ttaaagtata tctctctttt acgtaaagcc tgcgagatcc
    11401 tcttaagtat agcggggaag ccatcgttat tcgatattgt cgtaacaaat actttgatcg
    11461 gcgctatgcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
    11521 ttgcgcgctt ggcgagactc aaacaagata tattttgccc gccttacaga cgaaactagt
    11581 tggaggttat ggagcatact atcacgtggg cggccactgg tgagttacta caccccaggg
    11641 gcaacgttga tgctcctaaa aaactctggc tggacgcaag ccgtaacacc cgtgtcactt
    11701 cataatcgtt tgcaattcag ggcttgatct acacattacc ctgttatccc tattaattaa
    11761 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    11821 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    11881 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    11941 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12001 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    12061 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12121 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12181 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12241 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12301 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12361 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12421 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12481 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    12541 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    12601 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    12661 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    12721 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    12781 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    12841 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    12901 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    12961 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    13021 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    13081 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    13141 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    13201 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    13261 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    13321 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    13381 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    13441 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    13501 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    13561 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaacgaagca
    13621 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
    13681 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
    13741 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
    13801 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
    13861 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
    13921 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
    13981 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
    14041 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
    14101 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
    14161 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
    14221 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
    14281 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
    14341 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
    14401 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
    14461 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
    14521 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
    14581 cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg
    14641 agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct
    14701 atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat
    14761 gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc
    14821 tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc
    14881 tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc
    14941 tttgatattg gatcatacta agaaaccatt attatcatga cattaaccta taaaaatagg
    15001 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctcggcgcg
    15061 ccattaccct gttatcccta atatacaacg caaagaagct ggaaaattgg cgagggaatc
    15121 ctgtttctgt ctatccaaga atgggcatga ggtggcaacc gtcgtgctag cgtacagggt
    15181 gcactttgta accatttggg acaccggaca ctcgctgttt tcgaaattac cctttaagcg
    15241 cgggtattga accaggctta tgcccaagat cgtagcaagc atttaaatga cggtatcgat
    15301 aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagt taattaa
//
