LOCUS       sequence-217910-       10261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. 
            Lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10261)
  AUTHORS   Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen 
            DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, 
            Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE
  TITLE     BCL11A enhancer dissection by Cas9-mediated in situ saturating 
            mutagenesis.
  JOURNAL   Nature. 2015 Sep 16. doi: 10.1038/nature15521.
  PUBMED    26375006
REFERENCE   2  (bases 1 to 10261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Sep 16. doi: 10.1038/nature15521."
COMMENT     Sequence Label: lentiGuide-Crimson
FEATURES             Location/Qualifiers
     source          1..10261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13..690
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     411..430
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    706..1294
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(759..779)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1177..1188)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1366..1599
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1677..1798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1714..1733)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1768..1787
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1838..1973
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1993..2012)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1994..2012)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2022..2039)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2022..2038)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2031..2053)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2180..2635
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2267..2286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2477..2498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2661..2765
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2766..2834,2835..3626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2984..3003)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3797..4385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4286..4305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4539..4556
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4673..4694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4709..4714,4715..4732,4733..4739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4747..4763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4752..4774
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4771..4787
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4771..4787
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4806..4826
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4808..4826
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4854..5080
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5081..5261
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5308..5433
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5926..6159
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6344..6388
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6566..6814
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6566..6586
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6737..6756
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8696..8771
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    8827..8944
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9083..10261
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9314..10252
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10209..10229
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cgtacggcca ccatggatag cactgagaac gtcatcaagc ccttcatgcg cttcaaggtg
       61 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgtggg cgagggcaag
      121 ccctacgagg gcacccagac cgccaagctg caagtgacca agggcggccc cctgcccttc
      181 gcctgggaca tcctgtcccc ccagttcttc tacggctcca aggcgtacat caagcacccc
      241 gccgacatcc ccgactacct caagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg
      301 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
      361 accctcatct accacgtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtaatg
      421 cagaagaaga ctctgggctg ggagccctcc actgagcgca actacccccg cgacggcgtg
      481 ctgaagggcg agaaccacat ggcgctgaag ctgaagggcg gcggccacta cctgtgtgag
      541 ttcaagtcca tctacatggc caagaagccc gtgaagctgc ccggctacca ctacgtggac
      601 tacaagctcg acatcacctc ccacaacgag gactacaccg tggtggagca gtacgagcgc
      661 gccgaggccc gccaccacct gttccagtag acgcgttaag tcgacaatca acctctggat
      721 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
      781 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
      841 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
      901 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
      961 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     1021 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     1081 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     1141 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     1201 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     1261 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     1321 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     1381 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     1441 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1501 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1561 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     1621 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     1681 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     1741 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     1801 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     1861 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     1921 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     1981 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     2041 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     2101 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     2161 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2221 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2281 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     2521 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     2581 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     2641 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2701 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2761 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2821 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2881 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2941 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3001 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3061 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3121 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3181 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3241 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3301 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3361 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3421 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3481 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3541 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3601 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3661 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3721 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3841 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3901 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3961 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4021 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4081 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4141 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4201 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4261 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4321 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4381 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4441 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4501 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4561 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4621 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4681 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4741 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     4801 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     4861 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     4921 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     4981 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     5041 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     5101 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5161 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5221 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     5281 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     5341 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     5401 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     5461 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     5521 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     5581 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     5641 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     5701 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     5761 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     5821 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     5881 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     5941 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     6001 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     6061 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     6121 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     6181 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     6241 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     6301 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     6361 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     6421 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     6481 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     6541 cctcccaacc ccgaggggac ccagagaggg cctatttccc atgattcctt catatttgca
     6601 tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat
     6661 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
     6721 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
     6781 ggctttatat atcttgtgga aaggacgaaa caccggagac ggttgtaaat gagcacacaa
     6841 aatacacatg ctaaaatatt atattctatg acctttataa aatcaaccaa aatcttcttt
     6901 ttaataactt tagtatcaat aattagaatt tttatgttcc tttttgcaaa cttttaataa
     6961 aaatgagcaa aataaaaaaa cgctagtttt agtaactcgc gttgttttct tcacctttaa
     7021 taatagctac tccaccactt gttcctaagc ggtcagctcc tgcttcaatc attttttgag
     7081 catcttcaaa tgttctaact ccaccagctg ctttaactaa agcattgtct ttaacaactg
     7141 acttcattag tttaacatct tcaaatgttg cacctgattt tgaaaatcct gttgatgttt
     7201 taacaaattc taatccagct tcaacagcta tttcacaagc tttcatgatt tcttcttttg
     7261 ttaataaaca attttccata atacatttaa caacatgtga tccagctgct ttttttacag
     7321 ctttcatgtc ttctaaaact aattcataat ttttgtcttt taatgcacca atatttaata
     7381 ccatatcaat ttctgttgca ccatctttaa ttgcttcaga aacttcgaat gcttttgtag
     7441 ctgttgtgca tgcacctaga ggaaaaccta caacatttgt tattcctaca tttgtgcctt
     7501 ttaataattc tttacaatag cttgttcaat atgaattaac acaaactgtt gcaaaatcaa
     7561 attcaattgc ttcatcacat aattgtttaa tttcagcttt cgtagcatct tgttttaata
     7621 atgtgtgatc tatatatttg tttagtttca ttttttctcc tatatattca tttttaattt
     7681 taattcttta ataatttcgt ctactttaac tttagcgttt tgaacagatt caccaacacc
     7741 tataaaataa atttttagtt taggttcagt tccacttggg cgaacagcaa atcatgactt
     7801 atcttctaaa taaaatttta gtaagtcttg tcctggcata ttatacattc catcgatgta
     7861 gtcttcaaca ttaacaactt taagtccagc aatttgagtt aagggtgttg ctctcaatga
     7921 tttcattaat ggttcaattt ttaatttctt ttcttctggt ttaaaattca agtttaaagt
     7981 gaaagtgtaa tatgcaccca tttctttaaa taaatcttct aaatagtcta ctaatgtttt
     8041 attttgtttt ttataaaatc aagcagcctc tgctattaat atagaagctt gtattccatc
     8101 tttatctcta gctgagtcat caattacata tccataactt tcttcataag caaaaacaaa
     8161 atttaatccg ttatcttctt ctttagcaat ttctctaccc attcatttaa atccagttaa
     8221 agtttttaca atattaactc catatttttc atgagcgatt ctatcaccca aatcacttgt
     8281 tacaaaactt gaatatagag ccggattttt tggaatgcta tttaagcgtt ttagatttga
     8341 taattttcaa tcaattaaaa ttggtcctgt ttgatttcca tctaatctta caaaatgacc
     8401 atcatgtttt attgccattc caaatctgtc agcatctggg tcattcataa taataatatc
     8461 tgcatcatgt ttaataccat attcaagcgg tatttttcat gcaggatcaa attctggatt
     8521 tggatttaca acatttttaa atgtttcatc ttcaaatgca tgctcttcaa cctcaataac
     8581 gttatatcct gattcacgta atatttttgg ggtaaattta gttcctgttc cattaactgc
     8641 gctaaaaata atttttaaat cttttttagc ttcttgctct tttttgtacg tctctgtttt
     8701 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     8761 cgagtcggtg cttttttaag cttggcgtaa ctagatcttg agacaaatgg cagtattcat
     8821 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     8881 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     8941 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg
     9001 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
     9061 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     9121 cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg
     9181 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     9241 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     9301 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     9361 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
     9421 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     9481 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     9541 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     9601 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     9661 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     9721 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     9781 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     9841 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     9901 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     9961 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
    10021 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
    10081 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
    10141 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
    10201 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
    10261 a
//
