LOCUS       sequence-217943-       10474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prp5 in yCp50 plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Noble SM, Guthrie C
  TITLE     Identification of novel genes required for yeast pre-mRNA splicing 
            by means of cold-sensitive mutations.
  JOURNAL   Genetics. 1996 May;143(1):67-80.
  PUBMED    8722763
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "1996-05"; volume: "143(1)"; pages: "67-80"
COMMENT     Sequence Label: yCp50-PRP5
FEATURES             Location/Qualifiers
     source          1..10474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(437..456)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3989..4204
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4205..5008
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      7735..8188
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(8216..8238)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8324..8464
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8379..8398
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8479..8496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8650..9238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8730..8749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9409..10200,10201..10269))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10032..10051
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10270..10374)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10442..10460
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattctcat gtttgacagc ttatcatcga taaaaatttc aagatttttt tctacatcat
       61 atattatata ttattttgtt tttatttttc ctaataattc tctttttttt tttttattat
      121 gattgaatat gctttaaatt accaccctaa aaatttcaga actaactacg aaagtatata
      181 gcaccacgag tgagttaaat tctaaaaact agtatttagt actcttcaaa gacaagcttg
      241 cagcctttac gaccccctct ttaacttttt gctccagcaa ttctatactt aattgtatgt
      301 ctttctcatc ttsgcscttc gctrstwkrr rmsasyakyr mctwcrmrat yrwkkysttc
      361 agtttcccac ycgggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
      421 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
      481 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
      541 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
      601 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
      661 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
      721 gccgcactta tgactgtctt ctttatcatg caactcgtag gacagttcta tgatattctt
      781 gttccacaaa taaaataact cgaagcgaat gtgtgcgctt ccattcagga acagtaacca
      841 aaatggcagc taattgtaga atcagtgtat acgtatcaaa gttagtagtg atagccgcag
      901 gctggtcacc ctttgcctca acttttccgc acatttggat gggataaaga tctatagttt
      961 tttttggaaa gcagcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgy waamywmymm wwrsyagway
     1381 wtagtactct tcaaagacaa gcttgcagcc tttacgaccc cctctttaac tttttgctcc
     1441 agcaattcta tacttaattg tatgtctttc tcatcttggc cttcgatcaa taagtatagt
     1501 ttaggctcat cgttttcatt ctttggttcc ttcccttcag ggtaaaattt acccttgtta
     1561 gtaatactac atccggtttc atgcttgata aataataagg ttgtattctt tgtagcctcc
     1621 catcttacaa tctggggtaa atcattaata taaaccttgg cgtaaaagat gatcgacccg
     1681 tctgtagact ctttgaatag ttcataattg agtctcggaa taagcgcaga agactcagac
     1741 acactgtcat gcccttcgcg tggattgctg attttctttt ccaagtcatc gctcctctta
     1801 tcattctttt tgagctccaa atctttattt tgagcttcct ctcttttact tttgatattt
     1861 tccagtcctt tgccgccgaa ccctttcgac aacctaaact tacccttttt cataccggat
     1921 tcaaacttgg cactcatttc ctgaagttct tttgcttgca gagggtctaa agcttttatt
     1981 tcttcatcgc gcattgcctt gctcaagata taagcacctg acagttcatc atgcaataga
     2041 agagtaatag cagtaccgga gcgactacct ctggcagttc ttccagtagt atgaacatat
     2101 tgcgcgaaag ttttgacggc gttatagata ataaccaacg acacttcggg aacatttaaa
     2161 cctcttgaga gaacctctgt acagagaaga atactgttct tttctcgttt gaacttttct
     2221 aagttcataa gcctttcttg atatggctta cctgcatgaa tagcacaggt cacaattcca
     2281 gcatttaaca gcttttttga aatgaagtcg caaatattct gacttgatac aaatataatg
     2341 gctttagcat caacttcttc aacatcgctt gactgtccgt cattttcact ttgaacctcg
     2401 tcaaaaaatt cactgcgctc atggataagc tgcaccagat tgtcaaattt ttcatcttct
     2461 gaatggcata ttctaaactt ttgttttacg ttttcattaa ccattccctt tgaattaatc
     2521 gtaatagata ttggggaatg caaaactctt acggcaaaac tgcgtagttt gttcggaaaa
     2581 gttgcactaa atagaacaca ctgtttatcc ggtcgaacag ttttcatgat ttgcgttatt
     2641 tgaggttcaa aacctaaatc gaacagcctg tctgcctcat ccattactac gaacgttatt
     2701 cttttagtac taagtaattt cccatcattt agtgttaata tatcaataaa tcgtcccggt
     2761 gtggcaacaa caatctcagt gcctctttta agatcagtaa tctgcttttt catttcagaa
     2821 cctcctgtac agcatactga tctaatggat gtatctgctt ctgtgaattt agtaacttct
     2881 tcatgtattt gtaaagctag ctctctagtt ggagccagaa ttaaacccat gggccctgtt
     2941 tcatgttttg ataatggccg ttgagctttt acttgtctta gcaatggtaa aagataggag
     3001 atggtcttgc cggagccagt ttttgatatt cctataacat cacgacctga cataatagcg
     3061 ggaagagcct gggattgaat aggcgttaag gagccaaagt gcaacttttc tgtaattaaa
     3121 accatggtgt ccgttgatag tcccagttga gaccactttg tgactggttt ggggcaacct
     3181 gttcccttta ttttgatatt atccagacta agtctaagct cctcaacttc catctctgac
     3241 atcgaggaaa ctgtttcaga ttctatataa aaattttttt ggaacggctc aagctcttct
     3301 ggagagtaat aaatttgctt tacacgtttt tttgctttga gtttagcaat tttcgctata
     3361 ttagaattat ccgtattatc ttcaacatcc tcatcgtcgg ttcctccaag ttcaaacaat
     3421 tgatcctcta catcaagaat gtcgcctctg tcgtacatgc ctttactatt actcattttc
     3481 tcctccttca atgacgtcat aaactcatcc agtggatctt tttcattatc atgctctgga
     3541 gctttttgaa tgtgctcctt cgatctaaaa gaaactccta tttcagaatc atcgtcatca
     3601 ctgaagtcga aggatatttt cttctttacc ttacttctct tcgacttttt cttcgaaggc
     3661 ttaaccgata caggttcgtc agatttccta aattcatttt ttctcttccg aagctcttct
     3721 tttacacgtt cctgtctttc cgtaaacttt tcagtagtct gcttaccttc taaactgtta
     3781 gtaacaatgt cattgcgtga agtttgatgc tctttctgag catcaaattg tgcttttttt
     3841 tgtttccatt ttgctagttt tttcctcctt tcctccaata aagactccct attaatattt
     3901 tgcttcgaat caatagtttc catacctcaa ataaacccaa ggcaacagcg ttatgatttc
     3961 ccttctcgaa ttatkkggsg skgkrkkwtt caattcatca tttttttttt attctttttt
     4021 ttgatttcgg tttccttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg
     4081 aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg
     4141 aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata
     4201 aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc
     4261 caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg
     4321 taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa
     4381 aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc
     4441 attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa
     4501 tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac
     4561 gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga
     4621 agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct
     4681 atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt
     4741 tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat
     4801 tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac
     4861 cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag gactatttgc
     4921 aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata
     4981 tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact
     5041 aaactcacaa attagagctt caatttaatt atatcagtta ttacccggga atctcggtcg
     5101 taatgatttt tataatgacg aaaaaaaaaa aattggaaag aammcccccc ccccgcagcg
     5161 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
     5221 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
     5281 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
     5341 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
     5401 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
     5461 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
     5521 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
     5581 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     5641 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     5701 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     5761 ttcacgacca cgctgatgag ctttaccgct gggccattct catgaagaat atcttgaatt
     5821 tattgtcata ttactagttg gtgtggaagt cctaatatcg gtgatcaata tagtggttga
     5881 catgctggct agtcaacatt gagccttttg atcatgcaaa tatattacgg tattttacaa
     5941 tcaaatatca aacttaacta ttgactttat aacttattta ggtggtaaca ttcttataaa
     6001 aaagaaaaaa attactgcaa aacagtacta gcttttaact tgtatcctag gttatctatg
     6061 ctgtctcacc atagagaata ttacctattt cagaatgtat gtccatgatt cgccgggtaa
     6121 atacatataa tacacaaatc tggcttaata aagtctataa tatatctcat aaagaagtgc
     6181 taaattggct agtgctatat atttttaaga aaatttcttt tgactaagtc catatcgact
     6241 ttgtaaaagt tcacattagc atacatatat tacacgagcc agaaatagta acttttgcct
     6301 aaatcacaaa ttgcaaaatt taattgcttg caaaaggtca catgcttata atcaactttt
     6361 ttaaaaattt aaaatacttt tttatttttt atttttaaac ataaatgaaa taatttattt
     6421 attgtttatg attaccgaaa cataaaacct gctcaagaaa aagaaactgt tttgtccttg
     6481 gaaaaaaagc actacctagg agcggccaaa atgccgaggc tttcatagct taaactcttt
     6541 acagaaaata ggcattatag atcagttcga gttttcttat tcttccttcc ggttttatcg
     6601 tcacagtttt acagtaaata artayyammt yttaknnnag ttaactatga gataagcaag
     6661 tatcatctca tttcattacc tgaagtcgag taaacagaaa atccaattgt tgatgaacct
     6721 caatgactta gaactatcta tcggcagatc atataaagag gatttaggta cctagaggac
     6781 tgtacctgga gtatatatat atatatatat atattatctc aactatagtc catagaggtt
     6841 tctttcttga ggccttaaac tgctaaagaa tgatattggt ggaatgcaag caccaagcgc
     6901 tcttcttgcg tgactgttca tatacttcaa accaagaatg taactggcat tgacccatcc
     6961 aaaaccttca gtagctgccc ctttaaagtc agcaccttga ttaccgtatt ctgcttcaac
     7021 acgatgagga tctgttcctc ttgtgacatc atatttttca accacaatac cattataatc
     7081 gacaaaagcc tttgtcatca tgaaaagcca tctataagct agcctattcg ttacagttaa
     7141 ataaccataa gaacggaggc cttcccaagc aagaatttga tggggtgccc aaccaaatgg
     7201 atagtcccat tgtctaattg gtctcgaaat agaaattggg cctcgagaac gctccgtaca
     7261 tgcagctaaa cctccaagca tctctaactt gggtagtgct ttctccacca ttttctgtgc
     7321 ttgctccttc gtggcaagtc cagcccataa tgcccagaat gtagttgcgg attcgtatga
     7381 cgttctgtgc ttgatttttg tgttgtagtc aaagaaaagg tccggttaaa cggatctcgc
     7441 attgatgagg caacgctaat tatcaacata tagattgtta tctatctgca tgaacacgaa
     7501 atctttactt gacgacttga ggctgatggt gtttatgcaa agaaaccact gtgtttaata
     7561 tgtgtcactg tttgatatta ctgtcagcgt agaagataat agtaaaagcg gttaataagt
     7621 gtatttgaga taagtgtgat aaagttttta cagcgaaaag acgataaata caagaaaatg
     7681 attacgagga tacggagaga ggtatgtaca tgtgtattta tatactaagc tgccggcggt
     7741 tgtttgcaag accgagaaaa ggctagcaag aatcgggtca ttgtagcgta tgcgcctgtg
     7801 aacattctct tcaacaagtt tgattccatt gcggtgaaat ggtaaaagtc aaccccctgc
     7861 gatgtatatt ttcctgtaca atcaatcaaa aagccaaatg atttagcatt atctttacat
     7921 cttgttattt tacagatttt atgtttagat cttttatgct tgcttttcaa aaggcctgca
     7981 ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat ttcttagcat
     8041 ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc acacctccgc
     8101 ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt tctggcgtca
     8161 gtccaccagc taacataaaa tgttgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     8221 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     8281 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     8341 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     8401 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     8461 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8521 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8581 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8641 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8701 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8761 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8821 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8881 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8941 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9001 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9061 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9121 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9181 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9241 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9301 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9361 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9421 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9481 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9541 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9601 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9661 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9721 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9781 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9841 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9901 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9961 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10021 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10081 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10141 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10201 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10261 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10321 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10381 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10441 aaataggcgt atcacgaggc cctttcgtct tcaa
//
