LOCUS       sequence-217950-        3240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUC19 backbone with E. coli CRISPR array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Wright AV, Doudna JA
  TITLE     Protecting genome integrity during CRISPR immune adaptation.
  JOURNAL   Nat Struct Mol Biol. 2016 Oct;23(10):876-883. doi: 
            10.1038/nsmb.3289. Epub 2016 Sep 5.
  PUBMED    27595346
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3289";
            journalName: "Nat Struct Mol Biol"; date: "2016-10"; volume: "23"; 
            issue: "10"; pages: "876-883"
COMMENT     Sequence Label: pCRISPR
FEATURES             Location/Qualifiers
     source          1..3240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(716..1304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(796..815)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1475..2266,2267..2335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2098..2117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2336..2440)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2508..2526
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2564..2586)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2686..2705
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2899..2921
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2913..2930
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2914..2930
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcgggtgttc cccgcgccag cggggataaa ccgacaatca gggaacgatt gttgacactg
       61 taaaagtgtt ccccgtgcta gcggggataa accgacctga acgaaacgca aaatagtttc
      121 tttatggtgt tccccgcgcc agcggggata aaccgtggct gtggctttgt tttttgctct
      181 gatatatgtg ttccccgcgc cagcggggat aaaccggcgt ccgtcaaaga ggaagccaaa
      241 gccatgcagt gttccccgcg ccagcgggga taaaccggcg accacgcaga gcggcgacaa
      301 cctgacgttg tgttccccgc gccagcgggg ataaaccgct atagcagcac tccaccagct
      361 cgagcagcag gtgttccccg cgccagcggg gataaaccgc aaaaaaataa tatccggcag
      421 tctgtacggt agtgttcccc gcgccagcgg ggataaaccg tcggtttgcg cgtattgttg
      481 ttcggggtct atgtgttccc cgcgccagcg gggataaacc gtaggtaaat cacagctatt
      541 tgataagggc gtgtgtgttc cccgcgccag cggggataaa ccggagcctg acgagactgc
      601 tgaggccgtt ctgtcgagtt ccccgcgcca gcggggataa accaccatat aaccacatgt
      661 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      721 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      781 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      841 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      901 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      961 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1021 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1081 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1141 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1201 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1261 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1321 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1381 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1441 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1501 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1561 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1621 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1681 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1741 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1801 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1861 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1921 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1981 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2041 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     2101 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     2161 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     2221 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     2281 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     2341 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2401 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2461 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2521 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2581 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2641 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2701 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     2761 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2821 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     2881 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattctacc
     2941 tttttaatca atggattaag tactctttaa cataatggat gtgttgtttg tgtgatacta
     3001 taaagttggt agattgtgac tggcttaaaa aatcattaat taataatagg ttatgtttag
     3061 agtgttcccc gcgccagcgg ggataaaccg agcacaaata tcatcgctca aaccacttac
     3121 gggtgttccc cgcgccagcg gggataaacc gcctcgctgt aaattccaaa aacgatctct
     3181 atagtgttcc ccgcgccagc ggggataaac cgacagtacc ggagtttgac ggtgccaacg
//
