LOCUS       sequence-217977-       10562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prp2 in yCp50 plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Noble SM, Guthrie C
  TITLE     Identification of novel genes required for yeast pre-mRNA splicing 
            by means of cold-sensitive mutations.
  JOURNAL   Genetics. 1996 May;143(1):67-80.
  PUBMED    8722763
REFERENCE   2  (bases 1 to 10562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "1996-05"; volume: "143(1)"; pages: "67-80"
COMMENT     Sequence Label: yCp50-PRP2
FEATURES             Location/Qualifiers
     source          1..10562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(13..18,19..35,36..41)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(441..460)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        4077..4292
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4293..5096
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      7823..8276
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(8304..8326)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8412..8552
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8467..8486
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8567..8584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8738..9326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8818..8837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9497..10288,10289..10357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10120..10139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10358..10462)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10530..10548
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
       61 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
      121 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
      181 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
      241 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
      301 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
      361 gwcymmwycc rtccngygga tccwmtacgc cggacgcatc gtggccggca tcaccggcgc
      421 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      481 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      541 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      601 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
      661 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
      721 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
      781 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
      841 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
      901 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
      961 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     1021 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     1081 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg
     1141 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     1201 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     1261 ccgggsywcc tmgammtraa yrkaasccgg caaaaaacct tcaccgcctc cccctatttg
     1321 ttttatcgtc aattaaatct ttaaagattt gtggaaccat gtctatcaac cattcttcct
     1381 tagggataac tagacagtcc ctgataaatt ccttcgacgt tagcatcaat tgttgataaa
     1441 gcacatattt gcttggtctc tgcgcctttt ccttatgatt aacgaaaagt atactggtag
     1501 gatgaacact aacgttcagc ccacctgatg acctacccat ggtttgatag ccggtggggc
     1561 ctaattgtac aatattcatc ggaaacccgc tgatgaagca tcgagttatt ctagcattta
     1621 tgtagcccgc tatattgcct atcttcatcc tggcttggtc gttcttttca actaatccaa
     1681 ccttttcact gcacctgaat aattggtttc taatatttct taccctcaac atcgttttga
     1741 attgaatttt gtggtcttga caccaacttc tggaaaattt agagtttctc cattggttga
     1801 agatttccaa atacaatatg tgatcacttt ccacttcgct taacacactt gccgcagcat
     1861 ctcttttttg accaataaat aatgaaggtg tttcatgtag catcgaaact atggtcaaac
     1921 actcttctag tacaccttga cattgttcgt gcgtagcagc tgtataaagc acttttgcaa
     1981 attcaggttc acaaggaaac tcgcacatca ttttacctaa ccgagtgatt gtccctttgc
     2041 tatttaaggc acctaaaatg tataaatttt ccaaagactt ccgtaatgtg gggatactgg
     2101 gcttatccat caggggaaac tttattaggt cagtaacgcc aagcgataat aataataaca
     2161 ccgtattaga aagatttgtt cttgtgatct caggcttagg cataagttcc aactcgtgca
     2221 aatatgacca tttggtaaaa attctaaaac attttcccgg cccaacacga ccagccctac
     2281 cggcacgctg atcaactgaa gctcttgagc atggaactgt taaaagttgg gtcattccag
     2341 tggagggtac gtacgaattt tccttaacaa atccaggatc aatgacatat ctaataccat
     2401 caatggtcaa tgaagtttcc gcaatattag ttgccaaaac aactttccta caattttccg
     2461 gagtcggctg gaaaattttc aattgctgct cttggggtaa atttgcgtat ataggagtta
     2521 taatcatttg ttttgttcta gaacctaatt tagacatgat ctcctccagt ttggtttttg
     2581 ttctttcaat ttcttcttgc cccgttaaaa acaccaatat atcccccggt aaactttgcg
     2641 tagtatgaat ttgaaaaata gttgttatag cggcatgtat atagtttgcc tccggctgta
     2701 atgtgtagtg gatatctaca ggatatcttc tcccaggaac gttgaaaatg ggacaattat
     2761 caaaaaactc tgaaaatttt tttgcgttca ttgttgctga tgatataagc aattttaaag
     2821 tgggcctctg cggcaaaata tctttcagca aacctatcag gatatctgtg gctagagtac
     2881 gttcatgagc ttcatcaatc ataatacaag aatatttgct taatttggaa tctgtaagaa
     2941 attctcttag taacatacca tccgtcatat actttagaac cgttttattt ggggttgttt
     3001 tgtcttcaaa acggatctga taacccactt ccttcccaag cacaacattc atttcatctg
     3061 ctactcttgc agcaacagaa gtggccgcca cacgacgtgg ttgtgttatg gcaatctgta
     3121 actttccctg atcagtgaat ccatcttcta ccaaatattg gggtaactgt gtggttttac
     3181 cagaaccggt ttcacccatt ataatcaaaa cttgattttt tttgatctct tgtagtagct
     3241 catccttgta ttgatgtaca ggcaaaagtt ttcttgcctc ttgtatagtc agtatacgct
     3301 tttcctcagt ttctaaagct tgtgctagac gtgcctcata ttgaagtttc tcttcaggta
     3361 gtaaatcgtc ctcctcatta gtataatcaa tcattgcatc tgtgtcgaac acataatcgt
     3421 acttgtcaga gccttccaat gttatatcat cagggtgatc gctttgcccc gccatagcat
     3481 ttcgcaattg ttgttcttcc cacatgcgtt gtctgtttat tttgtcaatt tcttttgcat
     3541 tctttatttt atgagtaact tctacgttaa atttagaatt agaagaggcg tacttagttt
     3601 cattctcgga gtcacctagt agacccttct ttgtagtaag ttcattattt gacgttattt
     3661 tttcatcata ctttgatgac tttttttgtg atccaagttg ttctcctttt aaattgttct
     3721 tttctggacc ttggttcgta ttagaaaaaa ctttattggg gtcatagcgt gatcgtttta
     3781 gctgcaaggc agagttctta tccttggcct ctttgtcttc ttcctcaccc aaggaagaag
     3841 gctctattct tactgcattg tcattctgtc ttgtaacttc atacgttcgt ttcactcttc
     3901 gcttaccggt ttcagatgta atacttgaca tgtgttgcta tacacgcttg ttatctatat
     3961 agatgccaat taaacatata gcagctatcc gttattactt atacamyykw kkmakawcay
     4021 wtyywwmkyg tccgscwtcy ycagcagccg cacgcggcgc rkcggggggg gggggtttca
     4081 attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat ttttttgatt
     4141 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
     4201 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
     4261 gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
     4321 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
     4381 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
     4441 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
     4501 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
     4561 aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
     4621 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
     4681 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
     4741 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
     4801 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
     4861 gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg
     4921 gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca
     4981 ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac
     5041 gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa
     5101 ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat
     5161 atcagttatt acccgggaat ctcggtcgta atgattttta taatgacgaa aaaaaaaaaa
     5221 ttggaargaa amcccccccc ccgcagcgtt gggtcctggc cacgggtgcg catgatcgtg
     5281 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa
     5341 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca
     5401 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg
     5461 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca
     5521 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc
     5581 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca
     5641 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac
     5701 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg
     5761 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg
     5821 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgctgg
     5881 gccattctca tgaagaatat cttgaattta ttgtcatatt actagttggt gtggaagtcc
     5941 taatatcggt gatcaatata gtggttgaca tgctggctag tcaacattga gccttttgat
     6001 catgcaaata tattacggta ttttacaatc aaatatcaaa cttaactatt gactttataa
     6061 cttatttagg tggtaacatt cttataaaaa agaaaaaaat tactgcaaaa cagtactagc
     6121 ttttaacttg tatcctaggt tatctatgct gtctcaccat agagaatatt acctatttca
     6181 gaatgtatgt ccatgattcg ccgggtaaat acatataata cacaaatctg gcttaataaa
     6241 gtctataata tatctcataa agaagtgcta aattggctag tgctatatat ttttaagaaa
     6301 atttcttttg actaagtcca tatcgacttt gtaaaagttc acattagcat acatatatta
     6361 cacgagccag aaatagtaac ttttgcctaa atcacaaatt gcaaaattta attgcttgca
     6421 aaaggtcaca tgcttataat caactttttt aaaaatttaa aatacttttt tattttttat
     6481 ttttaaacat aaatgaaata atttatttat tgtttatgat taccgaaaca taaaacctgc
     6541 tcaagaaaaa gaaactgttt tgtccttgga aaaaaagcac tacctaggag cggccaaaat
     6601 gccgaggctt tcatagctta aactctttac agaaaatagg cattatagat cagttcgagt
     6661 tttcttattc ttccttccgg ttttatcgtc acagttttac agtwaaymar tayyammtyt
     6721 taknnnagtt aactatgaga taagcaagta tcatctcatt tcattacctg aagtcgagta
     6781 aacagaaaat ccaattgttg atgaacctca atgacttaga actatctatc ggcagatcat
     6841 ataaagagga tttaggtacc tagaggactg tacctggagt atatatatat atatatatat
     6901 attatctcaa ctatagtcca tagaggtttc tttcttgagg ccttaaactg ctaaagaatg
     6961 atattggtgg aatgcaagca ccaagcgctc ttcttgcgtg actgttcata tacttcaaac
     7021 caagaatgta actggcattg acccatccaa aaccttcagt agctgcccct ttaaagtcag
     7081 caccttgatt accgtattct gcttcaacac gatgaggatc tgttcctctt gtgacatcat
     7141 atttttcaac cacaatacca ttataatcga caaaagcctt tgtcatcatg aaaagccatc
     7201 tataagctag cctattcgtt acagttaaat aaccataaga acggaggcct tcccaagcaa
     7261 gaatttgatg gggtgcccaa ccaaatggat agtcccattg tctaattggt ctcgaaatag
     7321 aaattgggcc tcgagaacgc tccgtacatg cagctaaacc tccaagcatc tctaacttgg
     7381 gtagtgcttt ctccaccatt ttctgtgctt gctccttcgt ggcaagtcca gcccataatg
     7441 cccagaatgt agttgcggat tcgtatgacg ttctgtgctt gatttttgtg ttgtagtcaa
     7501 agaaaaggtc cggttaaacg gatctcgcat tgatgaggca acgctaatta tcaacatata
     7561 gattgttatc tatctgcatg aacacgaaat ctttacttga cgacttgagg ctgatggtgt
     7621 ttatgcaaag aaaccactgt gtttaatatg tgtcactgtt tgatattact gtcagcgtag
     7681 aagataatag taaaagcggt taataagtgt atttgagata agtgtgataa agtttttaca
     7741 gcgaaaagac gataaataca agaaaatgat tacgaggata cggagagagg tatgtacatg
     7801 tgtatttata tactaagctg ccggcggttg tttgcaagac cgagaaaagg ctagcaagaa
     7861 tcgggtcatt gtagcgtatg cgcctgtgaa cattctcttc aacaagtttg attccattgc
     7921 ggtgaaatgg taaaagtcaa ccccctgcga tgtatatttt cctgtacaat caatcaaaaa
     7981 gccaaatgat ttagcattat ctttacatct tgttatttta cagattttat gtttagatct
     8041 tttatgcttg cttttcaaaa ggcctgcagg caagtgcaca aacaatactt aaataaatac
     8101 tactcagtaa taacctattt cttagcattt ttgacgaaat ttgctatttt gttagagtct
     8161 tttacaccat ttgtctccac acctccgctt acatcaacac caataacgcc atttaatcta
     8221 agcgcatcac caacattttc tggcgtcagt ccaccagcta acataaaatg ttgcctcgcg
     8281 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     8341 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     8401 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     8461 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     8521 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     8581 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8641 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8701 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8761 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8821 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8881 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8941 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9001 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9061 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9121 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9181 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9241 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9301 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9361 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9421 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9481 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9541 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9601 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9661 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9721 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9781 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     9841 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9901 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9961 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10021 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10081 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
    10141 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10201 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10261 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10321 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10381 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10441 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10501 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
    10561 aa
//
