LOCUS       sequence-218042-        6103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa172 of VCre recombinase fused to ABI CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2583_pCAG-PV1-VCre-N172-L1-ABI-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2126..2149
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2150..2170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2180..2404
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2644..2663)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2709..2764
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2763..2782)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3125..3141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3125..3141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3138..3160)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3149..3165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3173..3179,3180..3197,3198..3203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3218..3239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3324..3458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3478..3612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3515..3534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3569..3588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3680..3697)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3851..4439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3931..3950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4610..5401,5402..5470))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5233..5252
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5471..5575)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5606..5985
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga tcgagaacca gctgagcctg ctgggcgact tttctggcgt
      721 gcggcccgac gatgtgaaaa ccgccattca ggccgcccag aaaaagggca tcaacgtggc
      781 cgagaacgag cagttcaagg ccgccttcga gcatctgctg aacgagttca agaagcggga
      841 agagagatac agccccaaca ccctgcggcg gctggaaagc gcctggacct gcttcgtgga
      901 ttggtgcctg gccaaccaca gacacagcct gcctgccacc cccgataccg tggaagcctt
      961 cttcatcgag cgggccgagg aactgcaccg gaacaccctg agcgtgtaca gatgggccat
     1021 cagccgggtg cacagagtgg ccggatgccc tgatccctgc ctggacatct acgtggaaga
     1081 tcggctgaag gccattgccc ggaagaaagt gcgggaaggc gaggccgtga agcaggccag
     1141 ccctttcaac gagcagcatc tgctgaagct gaccagcctg tggtacagaa gctccggagg
     1201 gtctggctcc ggatcaagtg gtggcagcgg tacccctttg tatggtttta cttcgatttg
     1261 tggaagaaga cctgagatgg aagatgctgt ttcgactata ccaagattcc ttcaatcttc
     1321 ctctggttcg atgttagatg gtcggtttga tcctcaatcc gccgctcatt tcttcggtgt
     1381 ttacgacggc catggcggtt ctcaggtagc gaactattgt agagagagga tgcatttggc
     1441 tttggcggag gagatagcta aggagaaacc gatgctctgc gatggtgata cgtggctgga
     1501 gaagtggaag aaagctcttt tcaactcgtt cctgagagtt gactcggaga ttgggtcagt
     1561 tgcgccggag acggttgggt caacgtcggt ggttgccgtt gttttcccgt ctcacatctt
     1621 cgtcgctaac tgcggtgact ctagagccgt tctttgccgc ggcaaaactg cacttccatt
     1681 atccgttgac cataaaccgg atagagaaga tgaagctgcg aggattgaag ccgcaggagg
     1741 gaaagtgatt cagtggaatg gagctcgtgt tttcggtgtt ctcgccatgt cgagatccat
     1801 tggcgataga tacttgaaac catccatcat tcctgatccg gaagtgacgg ctgtgaagag
     1861 agtaaaagaa gatgattgtc tgattttggc gagtgacggg gtttgggatg taatgacgga
     1921 tgaagaagcg tgtgagatgg caaggaagcg gattctcttg tggcacaaga aaaacgcggt
     1981 ggctggggat gcatcgttgc tcgcggatga gcggagaaag gaagggaaag atcctgcggc
     2041 gatgtccgcg gctgagtatt tgtcaaagct ggcgatacag agaggaagca aagacaacat
     2101 aagtgtggtg gtggttgatt tgaaggatta caaggacgat gacgataagc ccaagaaaaa
     2161 gcggaaggtg tgagaattcc tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2221 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2281 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2341 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     2401 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     2461 agcgtgtacc gctgggagtt cgtagacgga aacaaacgca gaatccaagc gcggccgccc
     2521 aggatacata gattaccaca actccgagcc cttccaccgc tagcgcactc ctcaggtgca
     2581 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     2641 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     2701 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     2761 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     2821 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     2881 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     2941 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     3001 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     3061 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     3121 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3181 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3241 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     3301 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccagttccg cccattctcc
     3361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     3481 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3541 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3601 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3661 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3721 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3781 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3961 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4021 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4081 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4141 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4201 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4261 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4321 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4381 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4441 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4501 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4561 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4621 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4681 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4741 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4801 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4861 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4921 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4981 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5041 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5101 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5161 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5221 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5281 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5341 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5401 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5461 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5521 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5581 ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca
     5641 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     5701 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     5761 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     5821 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     5881 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     5941 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     6001 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     6061 attttttaat tattttgtgc agcgatgggg gcgggggggg ggg
//
