LOCUS       sequence-218044-        3343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ATF1 in Ecoli off a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, 
            Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ
  TITLE     BioBits Explorer: A modular synthetic biology education kit.
  JOURNAL   Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. 
            eCollection 2018 Aug.
  PUBMED    30083608
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-08-1"; volume: "4(8):eaat5105. doi"; pages: " 
            10.1126/sciadv.aat5105. eCollection 2018 Aug"
COMMENT     Sequence Label: pJL1 ATF1
FEATURES             Location/Qualifiers
     source          1..3343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     617..636
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        617..635
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2328..2346)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2342..2389
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2407..3222)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3130..3149
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3214..3233
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga
      661 aataattttg ttaactttaa gaaggagata tacatatgaa tgaaattgat gaaaagaacc
      721 aagccccagt acagcaagaa tgcttaaaag aaatgattca gaacggacat gcccgtcgta
      781 tggggtcggt cgaggacctg tatgtggcgc ttaaccgtca gaacctttat cgtaactttt
      841 gtacatacgg ggagctgagt gactactgca cacgtgacca acttacactg gcgctgcgtg
      901 agatttgcct gaaaaatcct actcttttac atatcgtgtt gcccacgcgc tggccgaacc
      961 acgaaaacta ctatcgcagt tccgagtatt actcgcgtcc acatcccgtc cacgactaca
     1021 tctccgtatt acaagaattg aaacttagtg gagtagtgct taatgaacaa cctgagtact
     1081 cagcagtgat gaagcagatc ttagaagaat tcaagaattc aaagggcagt tatacagcga
     1141 aaatttttaa acttacaacc acactgacaa ttccttactt cggcccgact ggacccagtt
     1201 ggcgccttat ctgtcttccg gaggaacata ctgagaaatg gaagaaattt attttcgtca
     1261 gcaaccattg catgtcggac ggacgcagtt caattcattt tttccatgat ttacgcgacg
     1321 agttaaataa tattaagacc cctcctaaaa agttagacta tatttttaag tacgaggagg
     1381 attaccagtt gcttcgtaaa cttcccgaac caatcgaaaa ggtgatcgat ttccgccctc
     1441 cgtacttatt tatccccaaa agccttttat cgggattcat ttacaaccat cttcgtttca
     1501 gcagcaaagg ggtctgtatg cgcatggacg acgtggaaaa aactgacgat gttgtaacag
     1561 aaattattaa catttccccg actgaattcc aagccattaa ggcgaacatt aaatcaaata
     1621 tccaaggcaa atgtacgatt accccctttc tgcacgtgtg ttggttcgtg agccttcaca
     1681 agtggggcaa attttttaag cccttaaatt ttgagtggct tacggatatt ttcatccccg
     1741 cagattgtcg ttcccaatta cctgatgatg atgagatgcg tcagatgtac cgttacggtg
     1801 ccaacgtagg tttcattgac tttacgccat ggatttcaga atccgacatg aacgataaca
     1861 aagaaaattt ctggcctctg attgaacatt accatgaagt tatttcagaa gcactgcgta
     1921 ataagaaaca ccttcacgga ttagggttca acatccaagg atttgtacaa aagtacgtta
     1981 acatcgacaa ggtgatgtgc gatcgtgcaa ttggcaaacg tcgtggcggt acactgttgt
     2041 ccaatgtggg actgtttaat cagctggagg agcctgacgc taaatacagc atttgtgacc
     2101 ttgccttcgg tcagtttcaa ggcagctggc accaagcttt ttcattaggg gtgtgtagca
     2161 ctaatgtaaa agggatgaac attgtggtcg cgagcacgaa aaatgttgtg gggtcacagg
     2221 aatcattaga agaactgtgc agtatttaca aagctttgtt gctgggtcca taataagtcg
     2281 accggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     2341 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaagccaa
     2401 ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     2461 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     2521 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     2581 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     2641 gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac
     2701 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     2761 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     2821 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     2881 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
     2941 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     3001 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     3061 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     3121 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     3181 tcgcggcttc gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     3241 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
     3301 acatcagaga ttttgagaca caacgtgaga tcaaaggatc ttc
//
