LOCUS       sequence-218267-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Spacer as a Type 234 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK048
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(166..200)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       61 tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcaaac gactagagat
      121 ctatgtgagg atcctaactc gagatcgagc tgtgagacca gaccaataaa aaacgcccgg
      181 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
      241 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
      301 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
      361 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
      421 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
      481 ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag
      541 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
      601 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
      661 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
      721 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
      781 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
      841 ttgagcaact gactg
//
