LOCUS       sequence-218279-        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral system for reduced ectopic expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Dean KM, Roudot P, Reis CR, Welf ES, Mettlen M, Fiolka R
  TITLE     Diagonally Scanned Light-Sheet Microscopy for Fast Volumetric 
            Imaging of Adherent Cells.
  JOURNAL   Biophys J. 2016 Mar 29;110(6):1456-65. doi: 
            10.1016/j.bpj.2016.01.029.
  PUBMED    27028654
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2016.01"; journalName: "Biophys J"; date: 
            "2016-03-29- 29"; volume: "110"; issue: "6"; pages: "1456-65"
COMMENT     Sequence Label: pLVX-CMV300
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    112..168
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    527..642
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(905..949)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgcattcntt ttatgtttcn ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
       61 aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg tggatcccgg
      121 gcccgcggta ccgtcgactg cagaattcga agcttgagct cgagatctac tagttagcgc
      181 tagcggtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
      241 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
      301 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
      361 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
      421 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
      481 taactatcga taaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt
      541 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
      601 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaggcgatac cgtcgagatc
      661 cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt
      721 cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc
      781 cctgcctaac tctattcact atagaaagnn nagcaaaaac tattcttaaa cctaccaagc
      841 ctcctactat cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat
      901 tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt
      961 tttgcgattc ttcaattann ngtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
     1021 ctcntccagn cgtgngattc naatctnnnc ananattnat tantcnacta gcattncagg
     1081 nnnnncanng gngnaangan tttcnnancn ncccnatccc nngnnnnnnn nngnnnnnnt
     1141 tagg
//
