LOCUS       sequence-218345-       10355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral ProteinA-mCitrine expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, 
            Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau 
            K, Schnoegl S, Garner CC, Wanker EE
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology 
            for quantitative mapping of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Mol Syst Biol. 2018 Jul 11;14(7):e8071. doi: 10.15252/msb.20178071.
  PUBMED    29997244
REFERENCE   2  (bases 1 to 10355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2018-07-11"; volume: "14(7):e8071. doi"; pages: " 
            10.15252/msb.20178071"
COMMENT     Sequence Label: pLenti PA-mCit
FEATURES             Location/Qualifiers
     source          1..10355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        148..1359
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1196..1215
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1397..1570
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1571..1744
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             2086..2097
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    2573..3161
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2626..2646)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3044..3055)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3164..3180)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3165..3181)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3686..3866
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3892..3909)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3898..4122
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4168..4596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4255..4274)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4465..4486
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4605..4625)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4610..4939
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4790..4925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4852..4871
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4987..5034
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5053..5427
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5557..5678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5594..5613)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5648..5667
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5727..5743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5727..5743)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5740..5762)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5751..5767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5775..5781,5782..5799,5800..5805))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5820..5841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5958..5975)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6129..6717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6209..6228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6888..7679,7680..7748))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7511..7530
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7749..7853)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7928..7947)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8119..8498
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8500..8698
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8653..8673
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8716..8896
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8943..9068
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9561..9794
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9979..10023
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(10321..10355,1..83)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
       61 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
      121 taattaaccc gtgtcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg
      181 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
      241 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
      301 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
      361 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
      421 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
      481 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
      541 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
      601 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
      661 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
      721 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
      781 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
      841 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
      901 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
      961 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
     1021 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
     1081 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     1141 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     1201 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     1261 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     1321 tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc ccgggaatta
     1381 attcgctagc accatggtgg acaacaaatt caacaaagaa caacaaaacg cgttctatga
     1441 gatcttacat ttacctaact taaacgaaga acaacgaaac gccttcatcc aaagtttaaa
     1501 agatgaccca agccaaagcg ctaacctttt agcagaagct aaaaagctaa atgatgctca
     1561 ggcgccgaaa gtagacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt
     1621 acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga
     1681 cccaagccaa agcgctaacc ttttagcaga agctaaaaag ctaaatggtg ctcaggcgcc
     1741 gaaagtagac gcgaactcgg aattcggctc gggctcggta tcgaaaggtg aagagctgtt
     1801 tactggcgtc gtgcccattt tggtggaact tgacggtgac gtgaacggtc acaaattctc
     1861 agtctcggga gaaggcgagg gggacgcgac atatgggaaa ctcacactga agttcatctg
     1921 cacgactgga aagctgcctg tcccttggcc tacactcgtc acgacgttcg gttatggttt
     1981 gatgtgtttc gcaagatatc ctgaccacat gaaacagcat gatttcttta agagcgccat
     2041 gcccgaaggt tatgtccagg agaggactat cttctttaag gacgatggga actacaagac
     2101 tcgggcagag gtgaaatttg agggggacac gcttgtgaat agaatcgaac tcaaaggcat
     2161 cgatttcaag gaggatggaa acattctcgg acacaaactt gagtacaatt acaacagcca
     2221 caacgtgtat atcatggcgg acaaacagaa gaatggaatc aaagtgaact tcaagatcag
     2281 acacaatatc gaagatgggt cagtgcagct ggctgatcat taccagcaga atacgcccat
     2341 cggggacggt ccagtcttgt tgccggataa ccactacctt tcataccagt cgaagttgtc
     2401 caaagacccc aacgagaaga gggatcacat ggtgcttctt gaatttgtca cagccgctgg
     2461 ttgaaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat
     2521 ccagcacagt ggcggccgct cgagtctaga attcgatatc aagcttatcg ataatcaacc
     2581 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     2641 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     2701 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     2761 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     2821 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     2881 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     2941 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     3001 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3061 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3121 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     3181 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     3241 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     3301 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3361 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     3421 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     3481 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     3541 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     3601 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     3661 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     3721 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3781 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3841 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     3901 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3961 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     4021 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4081 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4141 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4201 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4261 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4321 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4381 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4441 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4501 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4561 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     4621 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4681 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4741 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4801 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4861 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4921 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4981 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     5041 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     5101 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     5161 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     5221 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     5281 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     5341 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     5401 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     5461 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     5521 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     5581 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5641 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     5701 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5761 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5821 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5881 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5941 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6001 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6061 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6121 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6181 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6241 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6301 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6361 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6421 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6481 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6541 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6601 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6661 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6721 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6781 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6841 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6901 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6961 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7021 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7081 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7141 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7201 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7261 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7321 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7381 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7441 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7501 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7561 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7621 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7681 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7741 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7801 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7861 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     7921 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     7981 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     8041 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     8101 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     8161 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     8221 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     8281 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     8341 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     8401 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     8461 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     8521 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     8581 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     8641 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     8701 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8761 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8821 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8881 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8941 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     9001 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     9061 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     9121 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     9181 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     9241 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     9301 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     9361 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     9421 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     9481 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     9541 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     9601 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     9661 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     9721 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     9781 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     9841 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9901 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     9961 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    10021 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    10081 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    10141 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    10201 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    10261 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
    10321 ttttaaaaga aaagggggga ttggggggta cagtg
//
