LOCUS       sequence-218381-        2612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Upstream homology arm, SpsC is involved in spore coat polysaccharide
            synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-A-spsCU
FEATURES             Location/Qualifiers
     source          1..2612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(560..577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(731..1319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(811..830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1490..2281,2282..2350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2113..2132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2351..2455)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2523..2541
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2545..2588)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2591..2610
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tccttactca ttgccgttaa tcggtaagga agaaattcag gaggttacag
       61 aaacgcttga atcgggatgg ctctctaaag gcccgaaggt tcagcagttt gaaaaagagt
      121 ttgccgcgtt tgtcggggcg aagcacgccg tggcggtgaa ttcatgtacc gcagctttat
      181 ttttggcttt gaaagcaaaa gggattgggc ccggggacga ggtcattacc tctccgctta
      241 cgttcagctc gaccgccaat acgattattc atacgggagc gacgcctgtg tttgcggata
      301 ttgacgaaaa cacgctcaat attgacccgg taaagcttga ggcggccgtc acaccgcgaa
      361 cgaaagcagt tgtgcccgtc cactttggcg ggcagtcctg cgatatggat gcaatattgg
      421 ctgttgccca aaatcatggt ttattcgtgc tggaggatgc ggcacatgcg gtatatacga
      481 cttataagca aagaatgatc ggttctatcg gggatgcaac ggcattcagc ttttacgtgc
      541 cagagacgct gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      721 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1141 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1621 tgataccgcg agagccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     1981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2461 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     2521 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     2581 atgatttctg gaattcgcgg ccgcttctag aa
//
