LOCUS       sequence-218402-        4964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H,
            Ross SE, Mostoslavsky R, Cohen HY, Hu LS, Cheng HL, Jedrychowski MP,
            Gygi SP, Sinclair DA, Alt FW, Greenberg ME
  TITLE     Stress-dependent regulation of FOXO transcription factors by the 
            SIRT1 deacetylase.
  JOURNAL   Science 2004 Mar 26;303(5666):2011-5. Epub 2004 Feb 19.
  PUBMED    14976264
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2004-03-26"; volume: "303(5666)"; pages: "2011-5. Epub 2004 
            Feb 19"
COMMENT     Sequence Label: FLAG-FOXO3a WT
FEATURES             Location/Qualifiers
     source          1..4964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..50)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             379..402
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2436..2452)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2436..2452)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    2567..2701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2604..2623)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2658..2677
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2760..2779)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3044..3061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3215..3803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3295..3314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3974..4765,4766..4834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4597..4616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4835..4939)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
       61 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaggaa
      121 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
      181 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
      241 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
      301 ctgagctatt ccagaagtag tgaagaggct tttttggagg agatctaagc ttggtaccga
      361 gctcggatcc tcaggatgga ctacaaggac gatgacgata aacccgggaa ttcggcagag
      421 gcaccggctt ccccggcccc gctctctccg ctcgaagtgg agctggaccc ggagttcgag
      481 ccccagagcc gtccgcgatc ctgtacgtgg cccctgcaaa ggccggagct ccaagcgagc
      541 cctgccaagc cctcggggga gacggccgct gactccatga tccccgagga ggaggacgat
      601 gaagacgacg aggacggcgg gggacgggcc ggctcggcca tggcgatcgg cggcggcggc
      661 gggagcggca cgctgggctc cgggctgctc cttgaggact cggcccgggt gctggcaccc
      721 ggagggcaag accccgggtc tgggccagcc accgcggcgg gcgggctgag cgggggtaca
      781 caggcgctgc tgcagcctca gcaaccgctg ccaccgccgc agccgggggc ggctgggggc
      841 tccgggcagc cgaggaaatg ttcgtcgcgg cggaacgcct ggggaaacct gtcctacgcg
      901 gacctgatca cccgcgccat cgagagctcc ccggacaaac ggctcactct gtcccagatc
      961 tacgagtgga tggtgcgttg cgtgccctac ttcaaggata agggcgacag caacagctct
     1021 gccggctgga agaactccat ccggcacaac ctgtcactgc atagtcgatt catgcgggtc
     1081 cagaatgagg gaactggcaa gagctcttgg tggatcatca accctgatgg ggggaagagc
     1141 ggaaaagccc cccggcggcg ggctgtctcc atggacaata gcaacaagta taccaagagc
     1201 cgtggccgcg cagccaagaa gaaggcagcc ctgcagacag cccccgaatc agctgacgac
     1261 agtccctccc agctctccaa gtggcctggc agccccacgt cacgcagcag tgatgagctg
     1321 gatgcgtgga cggacttccg ttcacgcacc aattctaacg ccagcacagt cagtggccgc
     1381 ctgtcgccca tcatggcaag cacagagttg gatgaagtcc aggacgatga tgcgcctctc
     1441 tcgcccatgc tctacagcag ctcagccagc ctgtcacctt cagtaagcaa gccgtgcacg
     1501 gtggaactgc cacggctgac tgatatggca ggcaccatga atctgaatga tgggctgact
     1561 gaaaacctca tggacgacct gctggataac atcacgctcc cgccatccca gccatcgccc
     1621 actgggggac tcatgcagcg gagctctagc ttcccgtata ccaccaaggg ctcgggcctg
     1681 ggctccccaa ccagctcctt taacagcacg gtgttcggac cttcatctct gaactcccta
     1741 cgccagtctc ccatgcagac catccaagag aacaagccag ctaccttctc ttccatgtca
     1801 cactatggta accagacact ccaggacctg ctcacttcgg actcacttag ccacagcgat
     1861 gtcatgatga cacagtcgga ccccttgatg tctcaggcca gcaccgctgt gtctgcccag
     1921 aattcccgcc ggaacgtgat gcttcgcaat gatccgatga tgtcctttgc tgcccagcct
     1981 aaccagggaa gtttggtcaa tcagaacttg ctccaccacc agcaccaaac ccagggcgct
     2041 cttggtggca gccgtgcctt gtcgaattct gtcagcaaca tgggcttgag tgagtccagc
     2101 agccttgggt cagccaaaca ccagcagcag tctcctgtca gccagtctat gcaaaccctc
     2161 tcggactctc tctcaggctc ctccttgtac tcaactagtg caaacctgcc cgtcatgggc
     2221 catgagaagt tccccagcga cttggacctg gacatgttca atgggagctt ggaatgtgac
     2281 atggagtcca ttatccgtag tgaactcatg gatgctgatg ggttggattt taactttgat
     2341 tccctcatct ccacacagaa tgttgttggt ttgaacgtgg ggaacttcac tggtgctaag
     2401 caggcctcat ctcagagctg ggtgccaggc tgaaggatcc actagttcta gataagtaat
     2461 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
     2521 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     2581 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     2641 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     2701 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     2761 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     2821 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     2881 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     2941 cttcctaatg caggagtcgc ataagggaga gcgtcgatat gcggtgtgaa ataccgcaca
     3001 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     3061 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3121 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3181 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3241 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3421 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3541 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3601 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3661 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3721 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3781 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3841 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3901 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3961 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4021 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4081 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4141 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4201 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4261 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     4321 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4381 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4441 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4501 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4561 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     4621 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4681 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4741 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4801 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4861 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4921 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acct
//
