LOCUS       sequence-218692-       10447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of BDNF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10447)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-BDNF
FEATURES             Location/Qualifiers
     source          1..10447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(22..24,25..27,28..738)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(22..24,25..27,28..738)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(67..88)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(328..347)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     675..696
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             754..813
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             1882..2253
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    2279..2867
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2332..2352)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2750..2761)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2870..2886)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2871..2887)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(3015..3048)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             3068..3248
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3286..3305)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3351..3406
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3405..3424)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3767..3783)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3767..3783)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3780..3802)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3791..3807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3815..3821,3822..3839,3840..3845))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3860..3881
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3940..4135
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3986..4121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4141..4275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4178..4197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4232..4251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4343..4360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4514..5101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4594..4613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5272..6063,6064..6132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5895..5914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6133..6237)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6268..6647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             6649..6906
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6953..7078
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7570..7687
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8047..8280
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8465..8509
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    8784..8804
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8831..8849
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    8872..8890
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    9036..9054
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    9116..9140
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9896..9920)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        9937..10447
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10366..10384
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ggggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
       61 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      121 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      181 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      241 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      301 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      361 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      421 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      481 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      541 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      601 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
      661 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      721 atggacgagc tgtacaaggc tagcggatca ggtcaatgta ctaactacgc tttgttgaag
      781 cttgccggcg atgttgaaag taaccccggt cctggatcca tgtccagact ggacaagagc
      841 aaagtcataa acggcgctct ggaattactc aatggagtcg gtatcgaagg cctgacgaca
      901 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
      961 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     1021 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc
     1081 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     1141 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     1201 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     1261 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     1321 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     1381 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     1441 ccggcctcga gcgaattcca gtacctgcca gatacagacg atcgtcaccg gattgaggag
     1501 aaacgtaaaa ggacatatga gaccttcaag agcatcatga agaagagtcc tttcagcgga
     1561 cccaccgacc cccggcctcc acctcgacgc attgctgtgc cttcccgcag ctcagcttct
     1621 gtccccaagc cagcacccca gccctatccc tttacgtcat ccctgagcac catcaactat
     1681 gatgagtttc ccaccatggt gtttccttct gggcagatca gccaggcctc ggccttggcc
     1741 ccggcccctc cccaagtcct gccccaggct ccagcccctg cccctgctcc agccatggta
     1801 tcagctctgg cccaggcccc agcccctgtc ccagtcctag ccccaggccc tcctcaggct
     1861 gtggccccac ctgcccccaa gcccacccag gctggggaag gaacgctgtc agaggccctg
     1921 ctgcagctgc agtttgatga tgaagacctg ggggccttgc ttggcaacag cacagaccca
     1981 gctgtgttca cagacctggc atccgtcgac aactccgagt ttcagcagct gctgaaccag
     2041 ggcatacctg tggcccccca cacaactgag cccatgctga tggagtaccc tgaggctata
     2101 actcgcctag tgacaggggc ccagaggccc cccgacccag ctcctgctcc actgggggcc
     2161 ccggggctcc ccaatggcct cctttcagga gatgaagact tctcctccat tgcggacatg
     2221 gacttctcag ccctgctgag tcagatcagc tcctaaagat ctgatcaagc ttatcgataa
     2281 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2341 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2401 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     2461 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     2521 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     2581 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2641 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     2701 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     2761 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     2821 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     2881 acctcgaggt tcgctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2941 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatgggatc
     3001 aattcaccat gggaataact tcgtatagca tacattatac gaagttatgc tgctttttgc
     3061 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3121 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3181 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3241 ctctagcagc atctagttga atcgccagtg tgtcgagcta gcagatcttt ttccctctgc
     3301 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     3361 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     3421 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     3481 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     3541 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     3601 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     3661 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     3721 cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc
     3781 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3841 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3901 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac
     3961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4141 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4201 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4261 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4321 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4381 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac ggatacctgt ccgcctttct
     4681 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4921 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5161 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     5281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     5341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     5401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     6181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     6241 atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat
     6301 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     6361 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     6421 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     6481 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     6541 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     6601 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggga ggcgtggcct
     6661 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     6721 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6781 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6841 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6901 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     6961 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     7021 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     7081 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     7141 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     7201 taatactggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     7261 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     7321 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     7381 agagcaaaac aaaagtaaga aaaaagcaca gcaagcagca ggatcttcag acctggaaat
     7441 tccctacaat ccccaaagtc aaggagtagt agaatctatg aataaagaat taaagaaaat
     7501 tataggacag gtaagagatc aggctgaaca tcttaagaca gcagtacaaa tggcagtatt
     7561 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     7621 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     7681 aaattttcgg gtttattaca gggacagcag aaatccactt tggaaaggac cagcaaagct
     7741 cctctggaaa ggtgaagggg cagtagtaat acaagataat agtgacataa aagtagtgcc
     7801 aagaagaaaa gcaaagatca ttagggatta tggaaaacag atggcaggtg atgattgtgt
     7861 ggcaagtaga caggatgagg attagaacat ggaaaagttt agtaaaacac cataaggagg
     7921 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7981 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8041 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8101 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8161 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8221 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8281 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8341 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8401 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     8461 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8521 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8581 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8641 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     8701 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     8761 acggtatcta gtgattatcg gatcaacttt gtatagaaaa gttgggtaca actcccacta
     8821 gacatatcct actctatcat tgatagagtg acccttccat agacatatcc tactctatca
     8881 ttgatagagt gacccttcca ctaggttcac ttttctctat cattgatagg gagtggtaaa
     8941 caattgatgg acaaaatcta tcaatgatag agagtgcatg ttactagtat tgttcttctc
     9001 tatcattgat agtgactttg cctaatcact tagcaactct atcattgata gagttctagt
     9061 attagcaagg cctttcctct atataaggaa gttcatttca tttggagaag atcccccaag
     9121 tttgtacaaa aaagcaggct tcaccatgac catccttttc cttactatgg ttatttcata
     9181 cttcggttgc atgaaggcgg cgcccatgaa agaagtaaac gtccacggac aaggcaactt
     9241 ggcctaccca ggtgtgcgga cccatgggac tctggagagc gtgaatgggc ccagggcagg
     9301 ttcgagaggt ctgacgacga catcactggc tgacactttt gagcacgtca tcgaagagct
     9361 gctggatgag gaccagaagg ttcggcccaa cgaagaaaac cataaggacg cggacttgta
     9421 cacttcccgg gtgatgctca gcagtcaagt gcctttggag cctcctctac tctttctgct
     9481 ggaggaatac aaaaattacc tggatgccgc aaacatgtct atgagggttc ggcgccactc
     9541 cgaccctgcc cgccgtgggg agctgagcgt gtgtgacagt attagcgagt gggtcacagc
     9601 ggcagataaa aagactgcag tggacatgtc tggcgggacg gtcacagtcc tagagaaagt
     9661 cccggtatcc aaaggccaac tgaagcagta tttctacgag accaagtgta atcccatggg
     9721 ttacaccaag gaaggctgca ggggcataga caaaaggcac tggaactcgc aatgccgaac
     9781 tacccaatcg tatgttcggg cccttactat ggatagcaaa aagagaattg gctggcgatt
     9841 cataaggata gacacttcct gtgtatgtac actgaccatt aagaggggaa gatagaccca
     9901 gctttcttgt acaaagtggt gataatcgaa ttccacgggg ttggggttgc gccttttcca
     9961 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
    10021 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
    10081 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
    10141 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
    10201 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
    10261 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
    10321 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
    10381 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
    10441 tccccag
//
