LOCUS       sequence-218744-        9944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the PDX1 trancription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9944)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPG-UBI-PDX1
FEATURES             Location/Qualifiers
     source          1..9944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..1229
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1066..1085
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1236..1260
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2118..2142)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2159..2669
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2588..2606
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      2685..2694
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2691..2693,2694..2696,2697..3410)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2691..2693,2694..2696,2697..3407)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2736..2757)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2997..3016)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3344..3365
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3426..4014
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3479..3499)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3897..3908)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4155..4188)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4208..4388
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4426..4445)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4491..4546
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4545..4564)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4907..4923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4907..4923)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4920..4942)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4931..4947
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4955..4961,4962..4979,4980..4985))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5000..5021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5080..5275
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5126..5261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5281..5415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5318..5337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5372..5391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5483..5500)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5654..6241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5734..5753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6412..7203,7204..7272))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7035..7054
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7273..7377)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7408..7787
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             7789..8046
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8093..8218
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8710..8827
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9187..9420
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9605..9649
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    9924..9944
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ttaattaagg gtgcagcggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
       61 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
      121 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
      181 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
      241 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
      301 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
      361 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag
      421 tagcgggctg ctgggctagc cggggctttc gtggccgccg ggccgctcgg tgggacggaa
      481 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact
      541 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct
      601 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
      661 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
      721 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
      781 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
      841 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
      901 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
      961 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     1021 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     1081 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     1141 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     1201 tctggccgtt tttggctttt ttgttagaca ggatcccaac tttgtacaaa aaagcaggct
     1261 tcaccatgaa cggcgaggag cagtactacg cggccacgca gctttacaag gacccatgcg
     1321 cgttccagcg aggcccggcg ccggagttca gcgccagccc ccctgcgtgc ctgtacatgg
     1381 gccgccagcc cccgccgccg ccgccgcacc cgttccctgg cgccctgggc gcgctggagc
     1441 agggcagccc cccggacatc tccccgtacg aggtgccccc cctcgccgac gaccccgcgg
     1501 tggcgcacct tcaccaccac ctcccggctc agctcgcgct cccccacccg cccgccgggc
     1561 ccttcccgga gggagccgag ccgggcgtcc tggaggagcc caaccgcgtc cagctgcctt
     1621 tcccatggat gaagtctacc aaagctcacg cgtggaaagg ccagtgggca ggcggcgcct
     1681 acgctgcgga gccggaggag aacaagcgga cgcgcacggc ctacacgcgc gcacagctgc
     1741 tagagctgga gaaggagttc ctattcaaca agtacatctc acggccgcgc cgggtggagc
     1801 tggctgtcat gttgaacttg accgagagac acatcaagat ctggttccaa aaccgccgca
     1861 tgaagtggaa aaaggaggag gacaagaagc gcggcggcgg gacagctgtc gggggtggcg
     1921 gggtcgcgga gcctgagcag gactgcgccg tgacctccgg cgaggagctt ctggcgctgc
     1981 cgccgccgcc gccccccgga ggtgctgtgc cgcccgctgc ccccgttgcc gcccgagagg
     2041 gccgcctgcc gcctggcctt agcgcgtcgc cacagccctc cagcgtcgcg cctcggcggc
     2101 cgcaggaacc acgatagacc cagctttctt gtacaaagtg gtgataatcg aattccacgg
     2161 ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     2221 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     2281 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     2341 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     2401 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
     2461 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
     2521 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
     2581 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
     2641 ttgaccgaat caccgacctc tctccccagg gggatccacc ggtcgccacc atggtgagca
     2701 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2761 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2821 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2881 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2941 tcttcaagtc cgccatgccc gaaggctatg tccaggagcg caccatcttc ttcaaggacg
     3001 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3061 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3121 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     3181 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3241 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     3301 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3361 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     3421 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3481 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3541 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3601 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3661 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3721 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3781 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     3841 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3901 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3961 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     4021 ttcccgcggt tcgctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4081 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatgggatc
     4141 aattcaccat gggaataact tcgtatagca tacattatac gaagttatgc tgctttttgc
     4201 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4261 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4321 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4381 ctctagcagc atctagttga atcgccagtg tgtcgagcta gcagatcttt ttccctctgc
     4441 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     4501 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     4561 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     4621 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     4681 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     4741 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     4801 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     4861 cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc
     4921 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4981 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5041 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac
     5101 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5161 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5221 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5401 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5461 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5521 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac ggatacctgt ccgcctttct
     5821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6061 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6721 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7021 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7381 atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat
     7441 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     7501 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     7561 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     7621 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     7681 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     7741 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggga ggcgtggcct
     7801 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     7861 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7921 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7981 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8041 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     8101 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     8161 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     8221 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     8281 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     8341 taatactggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     8401 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     8461 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     8521 agagcaaaac aaaagtaaga aaaaagcaca gcaagcagca ggatcttcag acctggaaat
     8581 tccctacaat ccccaaagtc aaggagtagt agaatctatg aataaagaat taaagaaaat
     8641 tataggacag gtaagagatc aggctgaaca tcttaagaca gcagtacaaa tggcagtatt
     8701 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     8761 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     8821 aaattttcgg gtttattaca gggacagcag aaatccactt tggaaaggac cagcaaagct
     8881 cctctggaaa ggtgaagggg cagtagtaat acaagataat agtgacataa aagtagtgcc
     8941 aagaagaaaa gcaaagatca ttagggatta tggaaaacag atggcaggtg atgattgtgt
     9001 ggcaagtaga caggatgagg attagaacat ggaaaagttt agtaaaacac cataaggagg
     9061 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     9121 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     9181 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     9241 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     9301 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9361 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9421 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9481 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9541 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9601 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9661 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9721 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9781 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     9841 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     9901 acggtatcta gtgattatcg gatcaacttt gtatagaaaa gttg
//
