LOCUS       sequence-218768-         471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with 
            repaired premature stop codon and puromycin resistance protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: pscALPS-SIV-AGM-TAN1 vpr
FEATURES             Location/Qualifiers
     source          1..471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 tctagagccg ccatggatta taaagatgac gatgacaaag actataaaga cgatgacgac
       61 aaagattaca aggacgacga cgacaaagcg gccgctatgg cggagggcag agacagcagg
      121 gagcgcaggc caggttggct cgaaatttgg gatctgtctc gggaaccttg ggacgagtgg
      181 ctgcgggaca tggtagccga actcaatcaa gaggcgcagc gccacttcgg acgagaactc
      241 ctctttcaag tatggaactt ttgtcaggaa gaaggtgagc gcaacggggc tcccatgatt
      301 gagagagctt ataggtatta tcgccttgta caaaaagccc ttttcgtcca ctttagatgt
      361 ggctgcaggc ggcgaacacc gtttgaaccg tatgaggaga ggagaaacgg agttgggggc
      421 ggaagggatg gaagagagcc gcctccaggt ttggcgtaac ccggggaatt c
//
