LOCUS       sequence-21881-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: FLAG-FLJ23445
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(788..805)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cgagctctct ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcatg ccagactaca aggacgatga tgacaagctc
      121 gagatgggag ccgtgactga cgacgaagtt atacggaaac gtcttctgat tgatggagat
      181 ggtgctgggg atgatcggag aattaatctc ctcgtgaaaa gcttcattaa atggtgcaac
      241 tctggatccc aagaggaagg gtatagccag taccaacgca tgctgagcac tctgtcccag
      301 tgtgaatttt caatgggcaa aacattgctg gtatatgata tgaatctcag agaaatggaa
      361 aattatgaaa aaatatacaa agaaatagaa tgtagtattg ctggagcaca tgaaaaaatt
      421 gctgagtgta aaaagcagat tcttcaagca aaacgaatac gaaaaaatcg acaagaatat
      481 gacgctttgg ccaaagtgat ccagcatcac ccagataggc atgagacact gaaggagcta
      541 gaggctctgg gcaaagaatt agagcatctc tcacatatta aagaaagtgt tgaagataag
      601 ctggaattga gacggaaaca atttcacgtt cttcttagta ccatccatga acttcaacag
      661 acattggaga atgatgacaa gctgtcagag gtggatgaag ctcaagaaag caccatggaa
      721 gcagacccta agccgtagag atccggtacc gtcgagtcta gagggcccgt ttaaacccgc
      781 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
      841 ccttccttga ccctggaagg tgccactccc act
//
