LOCUS       sequence-21882-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: pET41a-MED19
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..48
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             58..75
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             91..135
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             166..180
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 gnatctaatg gaggttactg tggttctggt catcaccatc accatcactc cgcgggtctg
       61 gtgccacgcg gtagtactgc aattggtatg aaagaaaccg ctgctgctaa attcgaacgc
      121 cagcacatgg acagcccaga tctgggtacc ggtggtggct ccggtgatga cgacgacaag
      181 agtcccatgg gatatcgggg atctaccatg gagaacttca cggcactgtt tggagctcag
      241 actgaccccc caccgccacc aagcgcccta ggctttgggc ctggaaagcc acccccgcca
      301 ccccctcctc ctccgggagg tggtccaggc gcagccccgc cctcgacggc aacctcggcc
      361 cccgcaggag cggataagtc gacggctgga agtggcccct tctacctgat gcgggaattg
      421 ccaggcagca cagagctgac gggcagcacc aatttaatca cacactacaa cctggagcag
      481 gcctataata agttctgtgg gaagaaagtg aaggagaagc taagtaactt cctgcctgac
      541 ctgccaggga tgattgatct ccctggctct catgacaaca gcagcctccg atccctcatt
      601 gagaagcctc ctattcttgg tggctctttt aatccaatca cagggaccat gctgtctggt
      661 ttccgcctcc acactggccn ttncccanaa atgtnttttt tnntnttttt tnnccccccc
      721 naaaanaana atagcatagc ccngaacgag cgcaccngga tctgttcccc anaacncttt
      781 gntcgatcca gaaagaaaga gaaaagagtc tgacgaaagg ananaaaaag acaananacg
      841 ctntcaaccc cggtgggant ttagcagaga agactcgtat ngnccccccc cctatgtact
      901 gttaagcgag atatgtngcn tatnttactg cnag
//
