LOCUS       sequence-218844-       13295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific expression of the GFP living color under the control
            of the Ngn3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13295)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 13295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLX-Ngn3-GFP
FEATURES             Location/Qualifiers
     source          1..13295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(721..745)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    763..1351
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(816..836)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1234..1245)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1494..1527)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             1547..1727
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1765..1784)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1830..1885
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1884..1903)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2246..2262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2246..2262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2259..2281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2270..2286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2294..2300,2301..2318,2319..2324))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2339..2360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2419..2614
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2465..2600
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2620..2754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2657..2676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2711..2730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2822..2839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2993..3581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3073..3092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3752..4543,4544..4612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4375..4394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4613..4717)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4748..5127
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             5129..5386
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5433..5558
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6050..6167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6527..6760
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6945..6989
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    7264..7284
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    13262..13286
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      join(13290..13295,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 gacccagttt cttgtacaaa gtggtgataa tcgaattccg ataatcaacc tctggattac
      781 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
      841 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
      901 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
      961 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     1021 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     1081 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     1141 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     1201 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     1261 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     1321 agtcggatct ccctttgggc cgcctccccg catcgggaat tcccgcggtt cgctttaaga
     1381 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     1441 gaagggctaa ttcactccca acgaagacaa gatgggatca attcaccatg ggaataactt
     1501 cgtatagcat acattatacg aagttatgct gctttttgct tgtactgggt ctctctggtt
     1561 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1621 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1681 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagttgaa
     1741 tcgccagtgt gtcgagctag cagatctttt tccctctgcc aaaaattatg gggacatcat
     1801 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     1861 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     1921 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     1981 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     2041 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     2101 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     2161 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     2221 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2281 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2341 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2401 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     2461 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2521 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     2581 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
     2641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2701 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc
     2761 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2821 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2881 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2941 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3001 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3061 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3181 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3421 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3541 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3601 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3661 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3721 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3781 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3841 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3901 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3961 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4021 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4081 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4141 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4201 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4261 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4321 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4381 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4441 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4501 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4561 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4621 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4681 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4741 ctgggtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     4801 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4861 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4921 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4981 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     5041 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     5101 tacgtattag tcatcgctat taccatggga ggcgtggcct gggcgggact ggggagtggc
     5161 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     5221 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5281 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     5341 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     5401 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     5461 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     5521 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     5581 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     5641 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatactggc ctgttagaaa
     5701 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     5761 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     5821 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     5881 aaaaagcaca gcaagcagca ggatcttcag acctggaaat tccctacaat ccccaaagtc
     5941 aaggagtagt agaatctatg aataaagaat taaagaaaat tataggacag gtaagagatc
     6001 aggctgaaca tcttaagaca gcagtacaaa tggcagtatt catccacaat tttaaaagaa
     6061 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     6121 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     6181 gggacagcag aaatccactt tggaaaggac cagcaaagct cctctggaaa ggtgaagggg
     6241 cagtagtaat acaagataat agtgacataa aagtagtgcc aagaagaaaa gcaaagatca
     6301 ttagggatta tggaaaacag atggcaggtg atgattgtgt ggcaagtaga caggatgagg
     6361 attagaacat ggaaaagttt agtaaaacac cataaggagg agatatgagg gacaattgga
     6421 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     6481 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     6541 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     6601 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     6661 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     6721 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     6781 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     6841 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     6901 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     6961 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     7021 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     7081 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     7141 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     7201 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcta gtgattatcg
     7261 gatcaacttt gtatagaaaa gttggatctc tcctttaaca gaggagaaac aggcccagag
     7321 gaaagatatg ctttcagtga cacaggagta gccgcagagc caggctgcct cccccacccc
     7381 acccccaatc ctcacgcaca tctgctgctc ttcccatcaa gttttgtcac cgaggctcgc
     7441 atctggaaac gtgcatgcaa cctgctaagg agacaagcag gctggagaag ctgccaagca
     7501 gaatcctccc ggtgtacccg cccgccccta gtgcgcctca ggtggacgcc aactcctggg
     7561 aatcccagga ggacgggaga gtcttcacca tccgagagtt caagagccca ggggtcaggc
     7621 gatgcccagc gagcaccccc agactttcca accctctccg ctccgtccag cggcccctgt
     7681 cccagccgcc ttttggcaag ccgcgctctt cctctgctgc cctgcgctct cctggctgcc
     7741 agcactgtga ggtggcgcaa cgcgccaaag ggcaagagct ccaaactgga gccgcctgga
     7801 ggtggcctcc cctcccccgc cgggttccgt tgtttatttc ttcctgggga gggaaaatca
     7861 cacagcctat tcgtttcccc agccgggatg ggcgcagagc cggacatatg ggcatctcgc
     7921 gctccgcatc tgtgtcatca gtcgcgtttt ctgtggatgc ctgcgtgttt gtacgtgtgt
     7981 gtgtatttgg ggccaagcgc tgggaacaaa tgtgcatgtg ttaaatttga caataaagtc
     8041 ctacagcgaa gctgctaaga ttggggcggt aggtgggggt ggaagacggg gggtgcgggg
     8101 tggaggagag ctaaactctc aaaaggcgag gccaaagcgc ctggcccagg tattatgttg
     8161 ggagaaggtg aggagagcag ctgctccttc tcgcggggtg tgtatgggag cgagggtgca
     8221 tgtgtccctg tgaagagtac acaacgatct gctcaacata tgtcgccaca aatcacagtg
     8281 tctctccctc ctcccacccc ccgctccctg cgcggctcct gaccgagcgc agagcagaga
     8341 ggggcgctga gaagaggagc cgcttcaaca ggtgtcttgg tgcgcaatgc accttttcac
     8401 gcattgacgg tctcagccgc cggcgaggat aatgtggaca ctttgtttcc aggcgccgcg
     8461 cgcgagctgc ggacgcctag gggaggcggc cctgagcagg gaggggggat ctggaaataa
     8521 agacctccgg ctctcctgag cgcccgggcc ctctcttccc tttgttcgaa gactccctgg
     8581 gccggagctc ccgtgtgcgc cctggggggt ggcatatggc gccctgtcac ctacccaaga
     8641 aaaagaaccg gccacccttt caagagcgca cgaggtccct gagcctgggg agggggagtc
     8701 tctgagggaa cggcttctct ccgaaagtgg gtgaggagcc cgtcttatct ctccccaccc
     8761 tgcctccacc atcccctcag ctggcgcagc agccgttggg tggggagctg cgcgctgggc
     8821 agcaccagcc tgcggaagcg ctccagctcc tggcagatga aatatgggtc tggtgagccc
     8881 ctggagacca tatgctttca ataaaagaag acaaatagga ccgagatagg ccttgcagcc
     8941 aacctgttat aaatgagtgg caattgggcc aatgtacacg ggggcagggc ccccgcgggg
     9001 aggcctgctt ggtgccaaat ccatgtgtca gcttctggga caggtgtgtc caggggccag
     9061 gggccaggtc tcccccctcc gggtttatca cggcaaaggt aatattgtgc taactatgag
     9121 taaacagtca ttgtgaaaat gagggagagt ttatcagggc gaactagttg gggggtggct
     9181 taacaataaa gttgttcgcc ttaggagcag gtgacggctg gctgcacgga ctcccgcggt
     9241 agatgttttc caaagcactc cactttacaa tctcttgtaa ttatttatta aacgaaatct
     9301 atttattatt attttagcaa acactggaga caggtggggc tttcttttcg tcgtctcctt
     9361 ttgtttgaag ggctgtttga agtggccagt ctcggcccgg gcagctcgct cgccagattt
     9421 ttgtcttccc ctctttcact gctgagctga aggcgagaga aagaatcccc ctcctctggc
     9481 aagccattag ctattcagct tcccatggcc cccctccctc cctgcacaat tctcaaagag
     9541 cccccaggca atgctaaaac tacccccgcc gacaagaagg caggaaggcg ggccgctgcc
     9601 ttccttcttt tccaatgtgc ccgcggtctc caggcgtctg gctaccgcct ctctccggga
     9661 cccctcagcc tccaggcaaa cttaggtctc aggaggtgta ggggtgcggg agtcctggag
     9721 gtcttagggg cggtggatga ccaggagctg caggaacaga ctctgctgtc tgcgggaaga
     9781 gccatttcac aaacactgcc ggtgcgcacg gagtcattac cgcctgcgcc tccgcagcca
     9841 aaggcacagg aattaacttt ggagaggggt ctatgtgtcc cctgcagctg agacagaggg
     9901 catggatcct caatctctaa tcgcctaaga ctccttgacc tccggccctg gcctcctggc
     9961 tggaagagga ctgcgggaaa tccttgcatc cctcaagaag gcggctgccc ccagccaccg
    10021 cgcgctcctc gccctggctg ctttttgcgc aaagcaggag tgatggagaa ctgcaagccc
    10081 tggagggccc tgagccggag gggtttgggg gaggctggat aagaattcca agcaggtccc
    10141 agaattctct acctatcaag aaagcttgaa agaatcccca gagtctttcc tggcatggga
    10201 agctcaccct gcgaaccttg tcagtatcca ttagccgctc caggcaggcc ccagccagag
    10261 tgagacctac ccctatctga ggtccactca cccccatctg aggtccaccg ccagatgaga
    10321 gtcatccaca ggcttctggg aaaccagagc ggtgcttgga tccctcgtgg ccagggttcc
    10381 cttcaaggtg cttagccagg tcaggaggcc ctagagaagc atggtttgga ttttctttcc
    10441 cagaccaaaa aagctccaag ttggttctct cccagtttct aacttgcagt taaataaatc
    10501 aggcaaggct ggcctatgag gcagacaagt gtgaagaagg agaaggagga ggagaaggag
    10561 aaggagaaag aagaagaagg aggagaagaa gaagaagaag aagaagaaga agaggaggag
    10621 gaggaggagg aggaggaagc agcagcagca gcagcagctt gaatggacag tggttcccct
    10681 tgcctagaaa atgggaccat tatttctttt ctaatctgac ccccagactc aggacttcct
    10741 ctattttctg cattttgggg tctcttgttt tgccttgaaa aaaaatgttt tctcccaaat
    10801 caaggagcag tagctggtgc aagggaaaat ctagggctag gagtcttaag atatgacttc
    10861 tatgtggttc tgatagaact tgctgtgtga ccttgagaga gtcactcccc ctctctgggc
    10921 cttgattttt tcatctttaa agaaggcctc aaattcccat tcttatgaga agaagacaag
    10981 ctcctagtga gtggtgacct aagggagcag ctgcagcaaa atgctaacct gacagtccca
    11041 gatggtccct ttattggttc tgaccctggt ctcaggcttc atttccccac agcaagggaa
    11101 ggagcctgct cacagagcac cagctaagat cagcaggacc gcgccacacc cccgcccagt
    11161 cctagagccc ccctctcgct ggttcctgag cataccaccc tcttccttgg aggaaaattt
    11221 gcccccaagc agcctaggcg gtaagaggct atcactaggg cagactcaca gacctacctc
    11281 atcccctcac cccaccctac agtctcgaag tcgggtcctg tcccctcctg cagtttccgg
    11341 gagactcagg atatctggac ctgctagaaa gagaagcctt cctcgcctaa ggagacttaa
    11401 accgggatac ttaaacctcc cgcctcggcg tcttcctcca ggcacgaccg ggtcaagaga
    11461 gagaagcgga agctgcaacc cctcactctg agtgaccgga agcagaagac cacgggatgt
    11521 cccaggcggg gacaagagga ggggctgggg aagaaaggag ggatgatgag ttcagagtcc
    11581 ctttggaaag gtttccagag agcgctacca gggacaaccc aaggggctgg ggaagtccct
    11641 gccttgtgct ctctgtgcga tgcccgagtg atgcagaggc agggggctgg agcaggtgac
    11701 tgctggcagc tgctgtctgt ctgtgattgg accggaggac taaggggaga aaaagtttat
    11761 cagcttctcc cagtgcctgc acgctgtggt agttcaaaag acacgagggg gaggggcaca
    11821 gcagctctgc ttcccagcgc cttgggagac tgaagtgaaa ggaacgcttg agcccaggag
    11881 ttcgagacca tcctgggcaa caaagcaaga ccgcccctca ccccatacaa aataaaaata
    11941 caaataaatt agccgggcac agtggcgcat gcctgtagtc tcagctactg ggaaggctga
    12001 agtgggagga tagcttgagc ccaggagatc aaggctgcag tgagctgtga ttgcaccact
    12061 gcagtccagc ctgggcgaca gaaggagacc gttttttggt tttgtttgtt cgtttaaaaa
    12121 aaaaaagaag caagagctca ctgtgaactc ctggttcctt cctcccctcc tcacacttcc
    12181 cagaactctt cctgtcacgg ttcctggcca gaacgctggg atactatcta caagctgtag
    12241 taggcttgta gtaatggaat gtccgcttga ggggtccccg cacagccaac cccggcctct
    12301 ggagtgggat ctatgggggt ggggttctaa gcgcctctgg ggagtgtgag gtagcatctc
    12361 agggtgtggc agaggctcgg acacccccaa aaggtctgtg aatggaaggg acataggcag
    12421 gatctctctc agtgatgtcc cctgtcttcc aggatgaaga gaggcagtga aacaccagga
    12481 gagcagggcg tcctttagaa ttcctggacc cttctccagg ctgctagtca ggacaatgag
    12541 ctcgtggttg tctttgccac tatcttcctg tgcgatttca gacaagccac ctccctcact
    12601 aagcctaaat ttccccatgt gtaacgtgca ggcattgtac cctagaggca tcaaagtccc
    12661 ctccaggaca gatgctaagg aaagataggc taggagcaaa gccgtctgag gtggcctgac
    12721 cagagccaca cgaggctctt ctcactgggc gaggctcttt gaggaaccga gagttgctgg
    12781 gacccagccc gccctcgaga gagcaaacag agcggcgctc ccctcccccg accccggccc
    12841 tttgtccgga atccagctgt gccctgcggg ggaggagcgg gctcgcgtgg cgcggcccca
    12901 gggccccggc gctgattggc cggtggcgcg ggcagcagcc gggcaggcac gctcctggcc
    12961 cgggcgaagc agataaagcg tgccaagggg cacacgactt gctgctcagg aaatccctgc
    13021 ggtctcaccg ccgcgcctcg agagagagcg tgacagaggc ctcggacccc attctctctt
    13081 cttttctcct ttggggctgg ggcaactccc aggcgggggc gcctgcagct cagctgaact
    13141 tggcgaccag aagcccgctg agctccccac ggccctcgct gctcatcgct ctctattctt
    13201 ttgcgccggt agaaaggtaa tatttggagg cctccgaggg acgggcaggg gaaagaggga
    13261 tccaactttg tacaaaaaag caggctcccg ccacc
//
