LOCUS       sequence-21886-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: pET32b-FLJ23445
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(693..707)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(723..767)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(774..791)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(801..818)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctccttcgg gcttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagctac
       61 ggcttagggt ctgcttccat ggtgctttct tgagcttcat ccacctctga cagcttgtca
      121 tcattctcca atgtctgttg aagttcatgg atggtactaa gaagaacgtg aaattgtttc
      181 cgtctcaatt ccagcttatc ttcaacactt tctttaatat gtgagagatg ctctaattct
      241 ttgcccagag cctctagctc cttcagtgtc tcatgcctat ctgggtgatg ctggatcact
      301 ttggccaaag cgtcatattc ttgtcgattt tttcgtattc gttttgcttg aagaatctgc
      361 tttttacact cagcaatttt ttcatgtgct ccagcaatac tacattctat ttctttgtat
      421 attttttcat aattttccat ttctctgaga ttcatatcat ataccagcaa tgttttgccc
      481 attgaaaatt cacactggga cagagtgctc agcatgcgtt ggtactggct atacccttcc
      541 tcttgggatc cagagttgca ccatttaatg aagcttttca cgaggagatt aattctccga
      601 tcatccccag caccatctcc atcaatcaga agacgtttcc gtataacttc gtcgtcagtc
      661 acggctccca tagatccgat atcagccatg gccttgtcgt cgtcgtcggt acccagatct
      721 gggctgtcca tgtgctggcg ttcgaattta gcagcagcgg tttctttcat accagaaccg
      781 cgtggcacca gaccagaaga atgatgatga tgatggtgca tatggccaga accagaaccg
      841 gccaggttag cgtcgaggaa ctctttcaac tgacctttag acagtgcacc cactttggtt
      901 gccgcccact tcaccgtttt tgacacacaa
//
