LOCUS       sequence-218861-        9332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express SST2 from the GAL1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Harrigan P, Madhani HD, El-Samad H
  TITLE     Real-Time Genetic Compensation Defines the Dynamic Demands of 
            Feedback Control.
  JOURNAL   Cell. 2018 Oct 18;175(3):877-886.e10. doi: 
            10.1016/j.cell.2018.09.044.
  PUBMED    30340045
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09"; journalName: "Cell"; date: "2018-10-18- 
            18"; volume: "175"; issue: "3"; pages: "877-886.e10"
COMMENT     Sequence Label: pHES944
FEATURES             Location/Qualifiers
     source          1..9332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        228..669
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    228..345
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     640..663
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(2794..2810)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2794..2810)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3945..3964)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3946..3964)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3971..3988)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3971..3987)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3980..4002)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4128..4583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4215..4234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4425..4446
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4939..4958)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5058..5080
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5118..5136)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5221..5325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5326..5394,5395..6186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5544..5563)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6357..6945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6846..6865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7099..7116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7233..7254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7269..7274,7275..7292,7293..7299)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7307..7323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7312..7334
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7331..7347
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7331..7347
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7368..7386
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccgggc ccttatattg aattttcaaa aattcttact ttttttttgg atggacgcaa
       61 agaagtttaa taatcatatt acatggcatt accaccatat acatatccat atctaatctt
      121 acttatatgt tgtggaaatg taaagagccc cattatctta gcctaaaaaa accctctctt
      181 tggaactttc agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc
      241 gccgagcggg cgacagccct ccgacggaag actctcctcc gtgcgtcctc gtcttcaccg
      301 gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct
      361 acaatactag cttttatggt tatgaagagg aaaaattagc agtaacctgg ccccacaaac
      421 cttcaaatta acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc
      481 ttatttctgg ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa
      541 atggaaaagc tgcataacca ctttaactaa tactttcaac attttcagtt tgtattactt
      601 cttattcaaa tgtcataaaa gtatcaacaa aaaattgtta atatacctct atactttaac
      661 gtcaaggaga aaaaactata ctcgagaata aaatggtgga taaaaatagg acgttgcatg
      721 aattatcttc caaaaatttc agcagaacgc cgaatggact gatctttacc aatgatttaa
      781 agacagtata ttccattttt ctaatttgtt tggatttgaa agagaaaaaa catagtagtg
      841 acactaagtc tttcttatta actgcattca ctaaacattt ccacttcact tttacatatc
      901 aggaagctat taaagcaatg ggacaattgg aactaaaagt ggatatgaac acaacttgta
      961 ttaacgtttc atataatatt aaacctagtt tggcgcgcca ccttcttaca ttatttatgt
     1021 cttctaagct actacataca ccgcaggaca gaactcgtgg cgagccaaaa gagaaagttc
     1081 ttttccaacc aacacctaag ggagttgctg ttttacaaaa gtatgttaga gatattgggt
     1141 tgaaaacaat gcctgatatt cttttgtcat cgtttaactc catgaaactt ttcacttttg
     1201 agagaagctc tgtcacggat tcaatcatcc atagtgatta tttgatccat attcttttca
     1261 ttaaaatgat gggcgctaag ccaaatgttt ggtctccaac aaacgcagat gacccattac
     1321 cttgcctatc ttcgttgttg gaatacacta ataatgatga tacatttact tttgagaaat
     1381 ctaagccaga acaaggatgg caagctcaga ttggaaacat cgatataaat gacttagaaa
     1441 gggtatcgcc attagctcat cgattcttta caaatccaga ttctgagtcg cacacgcaat
     1501 actacgtttc gaatgcaggt attaggttgt ttgaaaataa gacgtttggc acttccaaaa
     1561 aaatcgtgat taagtacacc tttacaacaa aggctatatg gcagtggata atggactgta
     1621 ctgatattat gcatgttaaa gaagcggtct cgttggcagc gttattttta aaaacggggt
     1681 tgattgtacc agttctttta caaccatcac gcactgacaa gaaaaagttt caaattagca
     1741 ggtcctcttt ttttaccttg tccaagcgtg gttgggattt ggtttcttgg acaggttgta
     1801 agagcaataa tattagagca ccaaatggta gcaccataga cctagatttc acattaaggg
     1861 gtcatatgac tgttcgtgat gaaaaaaaga cattggatga tagtgaaggt ttttctcaag
     1921 acatgcttat ctcttcgagt aatttaaata aactggacta tgtgctaact gatccaggta
     1981 tgagatattt attcaggcgc catttagaaa aggaactttg tgtagaaaat ttggatgtct
     2041 tcattgagat taagaggttt ttgaagaaaa tgacaattct gaaaaaattg atagattcta
     2101 agcattgtga taaaaaatct aataccagta cttcaaaaaa caatatcgta aaaacgattg
     2161 attcagcttt aatgaaacag gccaatgagt gccttgaaat ggcatatcac atttattcgt
     2221 cctatatcat gataggatca ccatatcaat taaatatcca tcataacttg cgtcagaata
     2281 tttctgacat catgttgcac cctcattctc cattatcgga acactttcct actaatctat
     2341 acgatccatc acctgcatcc gcggaatcag cagcttcatc aattagctca acggaggctg
     2401 acacgttagg tgaaccacct gaagtgagtc taaaaccttc aaaaaatctt tcaaatgaaa
     2461 actgttcatt taagaagcaa ggattcaaac atcagttaaa ggaatataaa cctgccccgt
     2521 taactctggc agaaacgcat tcgcctaatg catcagtaga aaattcacat acaattgttc
     2581 gttatggcat ggacaacacc caaaatgata ccaaatcggt tgattcattt ccagcaactt
     2641 tgaaggtcct caggaaatta tatcccctct tcgagatcgt tagcaatgaa atgtatagac
     2701 tgatgaataa cgattccttt caaaaattta cccagtcaga cgtatacaaa gatgctagcg
     2761 ctttaataga aatccaagaa aagtgctaat aaggatccac tagttctaga cacctgcaac
     2821 atgcgtgagt aaataagcgg ccgccaccgc ggtggagctc taagcaaata gctaaattat
     2881 atacgaatta atattatgat taagtgttta cgtgagtgcg atatttttat tactatctta
     2941 tacagttgta tatactctat aaaatgagtt gtctattaat taacgcgatg aatgctttct
     3001 gggtttacct ctccaacaac tctagtttac ttctcaatac attcaattgt atttgatttg
     3061 tcaatacttc atcattaatc aattctatag ttttgttttt ctcgtttatt tccaaattta
     3121 atgcatcaat tttattattc aatttgtcgt tgattttggt taatgatttt atggtttgat
     3181 ctctggcatt gattgtttgt gttagttttt cattattgat aattaaatta tttaagttag
     3241 ttatcaactc ggtgttttca agtttcaagt tttcaatttc tttagagttt attagatttg
     3301 tcaaagtttc tgaattgctt gattggtcct gtagaagagt atttgttgtt gtggataatt
     3361 gattcaattt ttgagacaat tgctggaagg cgttgaaata tctagcatca atctcatggt
     3421 ttttttcccg agagtctcgt agattcaatt gttttaatat atcttgggac cactcttgat
     3481 ttgaactcat ggaaattaaa ctgggtgttg tgttgtggtg taatgattgt accccctttg
     3541 cttataattg tgtggttccg atgctgactt actgggtatt atatgtgtgc ccaatagaaa
     3601 gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa
     3661 tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt
     3721 tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg
     3781 gcgagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga
     3841 ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga
     3901 ttcagaaggc cggccattta aatgtttaaa cggccacgct ggccccctat agtgagtcgt
     3961 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     4021 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4081 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
     4141 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4201 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4261 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4321 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4381 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4441 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4501 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4561 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     4621 atttcacacc gcatagggta ataactgata taattaaatt gaagctctaa tttgtgagtt
     4681 tagtattagg atctctaccc ttggcgaaaa gtcctctgcc aacaatgatg atatctgatc
     4741 caccacttac aacttcgtcg acggttctgt actgctgacc caatatgcgt atatatacca
     4801 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
     4861 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaaggt
     4921 ggtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     4981 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5041 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5101 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5161 aatggtttct tagacgtcgg cgcgccgagg acgtcaggtg gcacttttcg gggaaatgtg
     5221 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5281 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5341 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5401 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5461 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5521 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5581 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5641 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5701 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5761 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5821 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5881 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5941 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6001 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6061 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6121 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6181 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6241 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6301 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6481 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7021 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7081 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7141 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7201 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7261 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7321 caatttcaca caggaaacag ctatgaccat gattacgcca agctcggaat taaccctcac
     7381 taaagggaac aaaagctggg ccggccattt aaatgtttaa acggccacgc tggccatatt
     7441 tctaccaatc tctcaacact gagtaatggt agttataaga aagagaccga gttagggaca
     7501 gttagaggcg gtggagatat tccttatggc atgtctggcg atgataaaac ttttcaaacg
     7561 gcagccccga tctaaaagag ctgacaggga aatggtcaga aaaagaaacg tgcacccgcc
     7621 cgtctggacg cgccgctcac ccgcacggca gagaccaatc agtaaaaatc aacggttaac
     7681 gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt
     7741 actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct
     7801 tgtcacctta cgtacaatct tgatccggag cccgtcgctt tgagagaaat cagaagattc
     7861 caaaagtcta ctgaactttt gatcagaaag ttgcctttcc aaagactagt cagagaaatc
     7921 gcccaagatt tcaagaccga tctaagattc caatcctctg ccatcggtgc tctacaggaa
     7981 tccgttgaag cttacttggt ctctttgttc gaagacacca acttggccgc tatccacgct
     8041 aagcgtgtta ccatccaaaa gaaggatatc aagttggcta gaagattgag aggtgaaaga
     8101 tcctaagcac acagggattg ttgtacaatt actttttatg attttagata cacacaaatc
     8161 ttttctacga ttacctttca tttattttcg tttcatgttt tattaatcat tctttttgta
     8221 tatttcacat cgggtggggt ttttatatta aattttaata acataatctt ttaaagtttc
     8281 tactttctat cctgtatatt atttccatat ctatatctat attttcaagc ttgttcacat
     8341 ccttatcgga attgttccag cgtttttgta cagaaaatga tgttatggta acgagctata
     8401 tcagacctac tagtcttata gagctgtaca gagtcttgga aacataacca acacaccaac
     8461 aagatgtcat ttgattcgtt actcgacaag aatgataagc tggtaaaagt ttgcgggatt
     8521 caaaccgtcg aggctgccga aactgcgctt caagcaggcg ctgatttgat agggatcata
     8581 tgtgtcccca acaggaagcg gactatcgag agcgctgtgg ctcgtgaaat atccaaattg
     8641 attcacaaat caggtactac aaagctggtg ggggtgttca ggaatcaatc tgttgaggac
     8701 gtacatcggc tttctgagga atatgacctt gacataatcc aattacatgg tgatgaatca
     8761 tggccagagt actataacgt cattaagaaa ccaataatca aaagagtcat attccctaga
     8821 gatgtcgatg ttgtaacaca agtgtgtcaa agaaaaccct tggtatgtct accattgttc
     8881 gactccgagg caggcggtac aggtgaaaag ctggactggt ccagtatttc ctcctgggca
     8941 agcgaacaga ataacgtatt ttatatacta gcgggtggac taactgcaga aaacgtcatg
     9001 gaggcggtgg ggttgcctgg tgtaattggt gttgacgtct caggtggtgt agagactgac
     9061 ggcgtcaaag acaatgacaa gataattaaa tatgtacaaa atgcaaagaa acaatgaatt
     9121 acgaagagaa gtctcaatga ggttttacaa acaatcctat aatacatata tgccacttag
     9181 atatactttg aatactgaat atactgactc attatattct ttaattgtag tttctcaaaa
     9241 atattcatac aggttagcta tgaattcatg ctataatttc cattttgttc atgtaagata
     9301 cacataggga taaaatgtga taactaatca gc
//
