LOCUS       sequence-218959-       11373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding 
            sequence, two Cas9 and two type-IIs restriction enzyme recognition 
            sites next to the U6 promoter and gRNA sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T
  TITLE     Highly efficient RNAi and Cas9-based auto-cloning systems for C. 
            elegans research.
  JOURNAL   Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 
            10.1093/nar/gky516.
  PUBMED    29924347
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516."
COMMENT     Sequence Label: Ccas9LacZ
FEATURES             Location/Qualifiers
     source          1..11373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..23)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6..22)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        43..392
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     CDS             505..3552
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     misc_RNA        3654..3729
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     4001..4023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4061..4079)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4147..4251
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4252..4320,4321..5112)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4470..4489)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5283..5871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5772..5791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5998..6020
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6017..6033
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6017..6033
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    6053..6073
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6074..6673
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     CDS             join(6690..7733,7785..8855,8907..9932,9984..10946)
                     /codon_start=1
                     /product="S. pyogenes Cas9, codon optimized for C. elegans 
                     with synthetic introns"
                     /label=Cas9
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10962..10982
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10983..11009
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      11024..11347
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(11351..11371)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accaaaaaaa actagcaata
       61 aaggaataaa aaactgtaca ccttaaaggc gcacactctg ttttgcaaat tttattttta
      121 gttgtgaatt ttctgctgag acctgaaaat agcaacttta gtactactat aatttgtcaa
      181 ccttttcaaa aaaagcatgc aatttttgag aaactcttat aaaagctatt attaaaaaaa
      241 cacctttttt ccaaaattat tccacaaaaa atatgttatg aaatgcctac accctctcac
      301 acacactctt tatactactc tgtcaaactc acgagatgtc tgccgcctct tgtgttgccc
      361 ctatataaac acctcctatt gcgagatgtc ttggcaggtg gaggtcgacg gtatcgataa
      421 gcttagtctc cgaaggaacg tgtcacggtt gacagcagtc ctaggtataa tactagtgga
      481 ctttaagaag gagatataca tatggtcgtt ttacaacgtc gtgactggga aaaccctggc
      541 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      601 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
      661 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
      721 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
      781 accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
      841 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
      901 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
      961 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
     1021 gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag
     1081 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
     1141 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
     1201 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
     1261 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
     1321 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
     1381 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
     1441 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
     1501 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
     1561 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
     1621 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
     1681 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
     1741 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
     1801 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
     1861 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
     1921 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
     1981 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
     2041 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
     2101 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
     2161 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
     2221 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
     2281 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
     2341 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
     2401 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
     2461 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
     2521 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
     2581 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
     2641 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg
     2701 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc
     2761 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg
     2821 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
     2881 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
     2941 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac
     3001 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
     3061 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg
     3121 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta
     3181 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
     3241 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
     3301 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
     3361 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
     3421 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
     3481 ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg
     3541 tgtcaaaaat aataaccctt atcaacttga aaaagtggca ccgagtcggt gcttttttcc
     3601 gtgacacgtt ccttcggaga ctctagagcg gccgccaccg aagcttcacc tgcgttttag
     3661 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     3721 agtcggtgct ttttgtgaaa tttctggcgt aatagcgaag aggcccgcac cgatcgccct
     3781 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     3841 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtgcaatctg ctctgatgcc
     3901 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4021 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4081 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4141 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4201 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4261 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4321 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4381 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4441 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4501 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4561 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4621 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4681 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4741 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4801 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4861 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4921 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4981 attgcagcac tggggtcaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5041 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5101 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5161 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5221 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5281 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5341 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5401 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5461 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5521 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5581 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5641 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5701 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5761 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5821 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5881 aacgcggcct ttttacggtt cctggtcttt tgctggcctt ttgctcacat gttctttcct
     5941 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccagc
     6001 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct atcaactttg
     6061 tatagaaaag ttggcacctt tggtctttta ttgtcaactt ccattggttc ttccattgtt
     6121 tctgttaaat taatgaattt ttcataaaat aaagacatta tacaatataa aaatgaagaa
     6181 tttattgaaa ataaactgcc agagagaaaa agtatgcaac actcccgccg agagtgtttg
     6241 aaatggtgta cggtacattt tcgtgctagg agttagatgt gcaggcagca acgagagggg
     6301 gagagatttt tttgggcctt gtgaaattaa cgtgagtttt ctggtcatct gactaatcat
     6361 gttggttttt tgttggttta ttttgttttt atctttgttt ttatccagat taggaaattt
     6421 aaattttatg aatttataat gaggtcaaac attcagtccc agcgtttttc ctgttctcac
     6481 tgtttagtcg aatttttatt ttaggctttc aacaaatgtt ctaactgtct tatttgtgac
     6541 ctcacttttt atattttttt aatttttaaa aatattagaa gtttctagga taattttttc
     6601 gacttttatt ctctctaccg tccgcactct tcttactttt aaattaaatt gttttttttt
     6661 cagttgggaa acactttgct ctagaaaaaa tggacaaaaa atacagcatc ggccttgaca
     6721 tcggaacgaa ctccgtcgga tgggccgtca tcaccgacga gtacaaggtc ccatccaaga
     6781 agttcaaggt cctcggaaac accgaccgtc actccatcaa gaagaacctc atcggagccc
     6841 tcctcttcga ctccggagag accgccgagg ccacccgtct caagcgtacc gcccgtcgtc
     6901 gttacacccg tcgtaagaac cgtatctgct acctccaaga gatcttctcc aacgagatgg
     6961 ccaaggtcga cgactccttc ttccaccgtc tcgaggagtc cttcctcgtc gaggaggaca
     7021 agaagcacga gcgtcaccca atcttcggaa acatcgtcga cgaggtcgcc taccacgaga
     7081 agtacccaac catctaccac ctccgtaaga agctcgtcga ctccaccgac aaggccgacc
     7141 tccgtctcat ctacctcgcc ctcgcccaca tgatcaagtt ccgtggacac ttcctcatcg
     7201 agggagacct caacccagac aactccgacg tcgacaagct cttcatccaa ctcgtccaaa
     7261 cctacaacca actcttcgag gagaacccaa tcaacgcctc cggagtcgac gccaaggcca
     7321 tcctctccgc ccgtctctcc aagtcccgtc gtctcgagaa cctcatcgcc caactcccag
     7381 gagagaagaa gaacggactc ttcggaaacc tcatcgccct ctccctcgga ctcaccccaa
     7441 acttcaagtc caacttcgac ctcgccgagg acgccaagct ccaactctcc aaggacacct
     7501 acgacgacga cctcgacaac ctcctcgccc aaatcggaga ccaatacgcc gacctcttcc
     7561 tcgccgccaa gaacctctcc gacgccatcc tcctctccga catcctccgt gtcaacaccg
     7621 agatcaccaa ggccccactc tccgcctcca tgatcaagcg ttacgacgag caccaccaag
     7681 acctcaccct cctcaaggcc ctcgtccgtc aacaactccc agagaagtac aaggtaagtt
     7741 taaacatata tatactaact aaccctgatt atttaaattt tcaggagatc ttcttcgacc
     7801 aatccaagaa cggatacgcc ggatacatcg acggaggagc ctcccaagag gagttctaca
     7861 agttcatcaa gccaatcctc gagaagatgg acggaaccga ggagctcctc gtcaagctca
     7921 accgtgagga cctcctccgt aagcaacgta ccttcgacaa cggatccatc ccacaccaaa
     7981 tccacctcgg agagctccac gccatcctcc gtcgtcaaga ggacttctac ccattcctca
     8041 aggacaaccg tgagaagatc gagaagatcc tcaccttccg tatcccatac tacgtcggac
     8101 cactcgcccg tggaaactcc cgtttcgcct ggatgacccg taagtccgag gagaccatca
     8161 ccccatggaa cttcgaggag gtcgtcgaca agggagcctc cgcccaatcc ttcatcgagc
     8221 gtatgaccaa cttcgacaag aacctcccaa acgagaaggt cctcccaaag cactccctcc
     8281 tctacgagta cttcaccgtc tacaacgagc tcaccaaggt caagtacgtc accgagggaa
     8341 tgcgtaagcc agccttcctc tccggagagc aaaagaaggc catcgtcgac ctcctcttca
     8401 agaccaaccg taaggtcacc gtcaagcaac tcaaggagga ctacttcaag aagatcgagt
     8461 gcttcgactc cgtcgagatc tccggagtcg aggaccgttt caacgcctcc ctcggaacct
     8521 accacgacct cctcaagatc atcaaggaca aggacttcct cgacaacgag gagaacgagg
     8581 acatcctcga ggacatcgtc ctcaccctca ccctcttcga ggaccgtgag atgatcgagg
     8641 agcgtctcaa gacctacgcc cacctcttcg acgacaaggt catgaagcag cttaagcgtc
     8701 gtcgttacac cggatgggga cgtctctccc gtaagctcat caacggaatc cgtgacaagc
     8761 aatccggaaa gaccatcctc gacttcctca agtccgacgg attcgccaac cgtaacttca
     8821 tgcaactcat ccacgacgac tccctcacct tcaaggtaag tttaaacagt tcggtactaa
     8881 ctaaccatac atatttaaat tttcaggagg acatccaaaa ggcccaagtc tccggacaag
     8941 gagactccct ccacgagcac atcgccaacc tcgccggatc cccagccatc aagaagggaa
     9001 tcctccaaac cgtcaaggtc gtcgacgagc tcgtcaaggt catgggacgt cacaagccag
     9061 agaacatcgt catcgagatg gcccgtgaga accaaaccac ccaaaaggga caaaagaact
     9121 cccgtgagcg tatgaagcgt atcgaggagg gaatcaagga gctcggatcc caaatcctca
     9181 aggagcaccc agtcgagaac acccaactcc aaaacgagaa gctctacctc tactacctcc
     9241 aaaacggacg tgacatgtac gtcgaccaag agctcgacat caaccgtctc tccgactacg
     9301 acgtcgacca catcgtccca caatccttcc tcaaggacga ctccatcgac aacaaggtcc
     9361 tcacccgttc cgacaagaac cgtggaaagt ccgacaacgt cccatccgag gaggtcgtca
     9421 agaagatgaa gaactactgg cgtcaactcc tcaacgccaa gctcatcacc caacgtaagt
     9481 tcgacaacct caccaaggct gagcgtggag gactctccga gctcgacaag gccggattca
     9541 tcaagcgtca actcgtcgag acccgtcaaa tcaccaagca cgtcgcccaa atcctcgact
     9601 cccgtatgaa caccaagtac gacgagaacg acaagctcat ccgtgaggtc aaggtcatca
     9661 ccctcaagtc caagctcgtc tccgacttcc gtaaggactt ccaattctac aaggtccgtg
     9721 agatcaacaa ctaccaccac gcccacgacg cctacctcaa cgccgtagta ggaaccgccc
     9781 tcatcaagaa gtacccaaag ctcgagtccg agttcgtcta cggagactac aaggtctacg
     9841 acgtccgtaa gatgatcgcc aagtccgagc aagagatcgg aaaggccacc gccaagtact
     9901 tcttctactc caacatcatg aacttcttca aggtaagttt aaacatgatt ttactaacta
     9961 actaatctga tttaaatttt cagaccgaga tcaccctcgc caacggagag atccgtaagc
    10021 gtccactcat cgagaccaac ggagagaccg gagagatcgt ctgggacaag ggacgtgact
    10081 tcgccacagt ccgtaaggtc ctctccatgc cacaagtcaa catcgtcaag aagaccgagg
    10141 tccaaaccgg aggattctcc aaggagtcca tcctcccaaa gcgtaactcc gacaagctca
    10201 tcgcccgtaa gaaggactgg gacccaaaga agtacggagg attcgactcc ccaaccgtcg
    10261 cctactccgt cctcgtcgtc gccaaggtcg agaagggaaa gtccaagaag ctcaagtccg
    10321 tcaaggagct cctcggaatc accatcatgg agcgttcctc cttcgagaag aacccaatcg
    10381 acttcctcga ggccaaggga tacaaggagg tcaagaagga cctcatcatc aagctcccaa
    10441 agtactccct cttcgagctc gagaacggac gtaagcgtat gctcgcctcc gccggagagc
    10501 tccaaaaggg aaacgagctc gccctcccat ccaagtacgt caacttcctc tacctcgcct
    10561 cccactacga gaagctcaag ggatccccag aggacaacga gcaaaagcaa ctcttcgtcg
    10621 agcaacacaa gcactacctc gacgagatca tcgagcaaat ctccgagttc tccaagcgtg
    10681 tcatcctcgc cgacgccaac ctcgacaagg tcctctccgc ctacaacaag caccgtgaca
    10741 agccaatccg tgagcaagcc gagaacatca tccacctctt caccctcacc aacctcggag
    10801 ccccagccgc cttcaagtac ttcgacacca ccatcgaccg taagcgttac acctccacca
    10861 aggaggtcct cgacgccacc ctcatccacc aatccatcac cggactctac gagacccgta
    10921 tcgacctctc ccaactcgga ggagacggag gatccggacc accaaagaag aagcgtaagg
    10981 tctacccata cgacgtccca gactacgcct aaactagtga taaatgcaaa atcctttcaa
    11041 gcattccctt cttctctatc actcttcttt ctttttgtca aaaaattctc tcgctaattt
    11101 atttgctttt ttaatgttat tattttatga ctttttatag tcactgaaaa gtttgcatct
    11161 gagtgaagtg aatgctatca aaatgtgatt ctgtctgatg tactttcaca atctctcttc
    11221 aattccattt tgaagtgctt taaacccgaa aggttgagaa aaatgcgagc gctcaaatat
    11281 ttgtattgtg ttcgttgagt gacccaacaa aaagaggaaa ctttattgtg ccgccaagaa
    11341 aaaagtctca caactttatt atacatagtt gat
//
