LOCUS       sequence-21928-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Ariumi Y, Trono D
  TITLE     Ataxia-telangiectasia-mutated (ATM) protein can enhance human 
            immunodeficiency virus type 1 replication by stimulating Rev 
            function.
  JOURNAL   J Virol. 2006 Mar . 80(5):2445-52.
  PUBMED    16474151
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2006 Mar . 80(5):2445-52."
COMMENT     Sequence Label: pSUPER.MRE11i
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..266
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     187..206
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(339..355)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(340..356)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(384..402)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(423..439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(423..439)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(436..458)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    447..463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(471..477,478..495,496..501))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    516..537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(654..671)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(905..924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgcgggggc ggccgctctg aactagtgga tcccccgggc tgcaggaatt cgaacgctga
       61 cgtcatcaac ccgctccaag gaatcgcggg cccagtgtca ctaggcggga acacccagcg
      121 cgcgtgcgcc ctggcaggaa gatggctgtg agggacaggg gagtggcgcc ctgcaatatt
      181 tgcatgtcgc tatgtgttct gggaaatcac cataaacgtg aaatgtcttt ggatttggga
      241 atcttataag ttctgtatga gaccacagat ccccggcact gagaaacatg caattcaaga
      301 gattgcatgt ttctcagtgc ctttttggaa aagcttatcg ataccgtcga cctcgagggg
      361 gggcccggta ccagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca
      421 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      481 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      541 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      601 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
      661 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      721 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ngtggcgaaa cccgacagga
      901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      961 ctgcctacga acgccttc
//
