LOCUS       sequence-219906-        6657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Macian F, Garcia-Rodriguez C, Rao A
  TITLE     Gene expression elicited by NFAT in the presence or absence of 
            cooperative recruitment of Fos and Jun.
  JOURNAL   EMBO J. 2000 Sep 1. 19(17):4783-95.
  PUBMED    10970869
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2000 Sep 1. 19(17):4783-95."
COMMENT     Sequence Label: IL-2 promoter luciferase
FEATURES             Location/Qualifiers
     source          1..6657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    152..286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    380..514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1106..2758
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1172..1190)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2889..2954
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3084..3104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3529..3663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4434..4452)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4520..4624
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4625..4693,4694..5485)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4843..4862)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5656..6244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6145..6164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6398..6415
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6430..6570
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6496..6515)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcaggatcag
       61 ccatatcaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      121 cctgaaacat aaaatgaatg caattgttgt taacttgttt attgcagctt ataatggtta
      181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      241 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcagcc atatcacatt
      301 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      361 aatgaatgca attgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      421 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      481 caaactcatc aatgtatctt atcatgtctg gatccaagct cagatccaag cttgtcgaca
      541 gatctatcac cctgtgtgca attagctcat tgtgtagata agaaggtaaa accatcttga
      601 aacaggaaac caatatcctt cctgtctaat caacaaatct aaaagattta ttcttttcat
      661 ctatctcctc ttgcgtttgt ccaccacaac aggctgctta caggttcagg atggttttga
      721 caaagagaac attttcatga gttacttttg tgtctccacc ccaaagagga aaatttgttt
      781 catacagaag gcgttcattg tatgaattaa aactgccacc taagtgtggg ctaacccgac
      841 caagagggat ttcacctaaa tccattcagt cagtgtatgg gggtttaaag aaattccaga
      901 gagtcatcag aagaggaaaa acaaaggtaa tgctttttgc cacacaggta gactctttga
      961 aaatatgtgt aatatgtaaa acatcgtgac acccccatat tatttttcca gcattaacag
     1021 tataaattgc ctcccatgct gaagagctgc ctatcaccct tgctaagctt ggatctgagc
     1081 ttggcattcc ggtactgttg gtaaaatgga agacgccaaa aacataaaga aaggcccggc
     1141 gccattctat cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag
     1201 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac
     1261 gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
     1321 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
     1381 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
     1441 tgaattgctc aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg
     1501 gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat
     1561 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     1621 acctcccggt tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat
     1681 tgcactgata atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca
     1741 tagaactgcc tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     1801 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     1861 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     1921 gtttttacga tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt
     1981 ttcattcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     2041 tgcttctggg ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca
     2101 tcttccaggg atacgacaag gatatgggct cactgagact acatcagcta ttctgattac
     2161 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     2221 ggttgtggat ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt
     2281 cagaggacct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     2341 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     2401 cttcatagtt gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc
     2461 tgaattggaa tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct
     2521 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     2581 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     2641 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     2701 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa
     2761 tgtaactgta ttcagcgatg acgaaattct tagctattgt aatactctag aggatctttg
     2821 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     2881 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     2941 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     3001 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     3061 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     3121 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     3181 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     3241 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
     3301 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
     3361 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
     3421 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3481 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3541 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3601 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     3661 atcgatcccc aggaagctcc tctgtgtcct cataaaccct aacctcctct acttgagagg
     3721 acattccaat cataggctgc ccatccaccc tctgtgtcct cctgttaatt aggtcactta
     3781 acaaaaagga aattgggtag gggtttttca cagaccgctt tctaagggta attttaaaat
     3841 atctgggaag tcccttccac tgctgtgttc cagaagtgtt ggtaaacagc ccacaaatgt
     3901 caacagcaga aacatacaag ctgtcagctt tgcacaaggg cccaacaccc tgctcatcaa
     3961 gaagcactgt ggttgctgtg ttagtaatgt gcaaaacagg aggcacattt tccccacctg
     4021 tgtaggttcc aaaatatcta gtgttttcat ttttacttgg atcaggaacc cagcactcca
     4081 ctggataagc attatcctta tccaaaacag ccttgtggtc agtgttcatc tgctgactgt
     4141 caactgtagc attttttggg gttacagttt gagcaggata tttggtcctg tagtttgcta
     4201 acacaccctg cagctccaaa ggttccccac caacagcaaa aaaatgaaaa tttgaccctt
     4261 gaatgggttt tccagcacca ttttcatgag ttttttgtgt ccctgaatgc aagtttaaca
     4321 tagcagttac cccaataacc tcagttttaa cagtaacagc ttcccacatc aaaatatttc
     4381 cacaggttaa gtcctcattt aaattaggca aaggaattct tgaagacgaa agggcctcgt
     4441 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4501 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4561 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4621 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4681 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4741 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4801 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4861 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4921 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4981 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5041 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5101 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5161 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5221 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5281 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5341 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5401 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5461 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5521 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5581 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5761 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6301 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6361 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6421 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     6481 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     6541 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     6601 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctcc
//
