LOCUS       sequence-219953-        6488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pSW454 - pUFO
FEATURES             Location/Qualifiers
     source          1..6488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6074..6093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6075..6093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6103..6125)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6319..6338)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6438..6460
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgatatcgaa ttctctgttt taattgcccc
     2281 acttctacaa ttgaacaaca cctcccccaa aaaaaaacta tcgctcatct ccacggtttt
     2341 aatttttttc tgcaactatg atatccaatt ttcctcgacc atacataatt ataatctttg
     2401 ctgatatacc aaaattttag aattttttta ttaaatacag tttacagcga tgaagacatt
     2461 aattttggca tttgtatttt gcgggagaat acaaacaaca aggtcagtca tctacttcac
     2521 atgcattcaa atttatattc ttttatgtta gacaatgtta taagcctttg tttcttaatt
     2581 cgtaatatgg tgttattttc caaatctata atttggctta ttagtttgca gttgaacaag
     2641 ctagggcatg tatagatgtg tctttatagt tattagcgta tacaaagaac aaattggacg
     2701 atagatgtgt ctttcaataa tttcttattt tttccttaca atgttttttt ttgtctttct
     2761 gtatttgatg ttttcttcat aataattgca ttttctatcc ctctcgaaaa ttagtacctc
     2821 aatcaaattg atctcaagtc aagttctgga cacaactacc ttttatcttt ttgaatttaa
     2881 tgttaaatta tattcaaaat aaaaataaac gtttttcttt gaatttaact ctatttgttt
     2941 tcttttggaa agttgatgag tgattgtcgg taactatact aaaaatggtt ttgcattttt
     3001 ctataggtgt ggtatgcctt ggtacgtgga ggaaacaagt tagacacata agcacatatg
     3061 tactagacgc aaataatgtc acaaaaacgg tttaaaatat ctggttttca gtttgcatgt
     3121 cgtacttata accgaacgtg tgtaaagttc tcatgtcata taccagttct gttaaactaa
     3181 tgaaatatgt tcagaggttt aaccactata actattagac ccgatttcaa ttaatcaaga
     3241 aatatccaaa ctttttaata tatgcattat gctttacaaa attgtttgat cttctaatga
     3301 tgtaaagaca tttacaaaat ataattattt aattgattct aactaatttg tccgcgcata
     3361 acgctggccg taaactagtt cttcttacaa attacaatat caatttccac tttgggccgg
     3421 aaatttatat ccataactct actacgtacc aattaacaca tgtgtgtaaa taaaactaca
     3481 taatggcatg aatggagttt cgaatcagtt aatgtgggcg acaaaataaa atcagattat
     3541 gttttattat taagtttaga aagagatgct tcattaaaag tttatgcttt aatgttaaat
     3601 cagactattt attatgggta cgacttgtaa gcgtaattca aagttaaaca aaacctagtt
     3661 gggaaatggg aagatagatg atttgataaa aacaagacac tgtttggttt cagagacttc
     3721 ctttagcatc aaaacacaaa caaaagcgaa gcctcttgag ttactgcaaa tagagaatat
     3781 tattaccctt ttgcgacttg tcagcttcag atattctcac ttgtattatt attttcacgg
     3841 taaacaatgc cttaaataag aaaccctgat tggacttttg atctgactct actcttcact
     3901 ctttcttctt ctttattttc agtcatgatg tctctctaac cctaatctca aaaaatccaa
     3961 actcctttta tttatttcta aaccttgatt atagctagca atgattaata taagaatttt
     4021 ttttctggat aaagaattaa ttagaaattg agttgtaaat gttttgtgat gtctaaaatt
     4081 cttttgtttg caaattagat gtaaattgat atatttgaga tttgtatgaa agctagtttt
     4141 attttcccta aacaagaagt tcattatctt ggacttggag gttttagagt ttgaaagagt
     4201 ttacaattta taagaaaaaa taatcactat atatatatat atagtgtata taatgaattg
     4261 tttcacatta aattgcaaca acatcaaata agggtaacat actaacatat agtttgtttg
     4321 tttactcttt aaaaaaaggg gataaactaa gagctatttc tgctataatt taggaacaaa
     4381 ctggatcaca tgacaaaaat gcatcataat tcatattaaa ttttgtgtat atctatttct
     4441 catgtttaga aataacattc ttgtgtgtta tacatgttat cagtttttct tcctagatgg
     4501 aagttttatt gttggagtct tttaaaacca tactcactat gttcctcttt atttgatgtt
     4561 ttggatttta gatagaaatt aataaaaaat atgttttcta tttttataaa attatttttt
     4621 tgttagttta gtaattttat ttttcttttt ttattattag tcacaagcaa aaataataac
     4681 aatattttta taaaacatta attttggtcg aacaagtaaa aataactcaa aacatcaaat
     4741 aattagaaac taaaaaagta gatattgtca aattttgtgt tgagttcgaa taagataatg
     4801 tgctctcctc caacaaaatt atttagaata aatgcacttc tatgacatta gagaaccaac
     4861 taatttattt agaataaaac acacatatat attaacatat aaagtaattc taattggctt
     4921 gatataatat ataagtaaaa aaatgatctt aataatctct agttttcttg ggttgatctc
     4981 cacgagtaca atttgactga ccattataga agttgagaag cgtgcatgta ataaaagttg
     5041 tatattacaa attagagagg aaaaagaaag aaagaaaaaa gatttgaaga tgtgatcaag
     5101 tgtgaaaagt attggagtag tctccaaatt aataatttcg atgctgggca ttgacaagat
     5161 aactctgaag ctctcaactt taagaccatc acttcctctg ccaccatttt cacgtttacc
     5221 caaacacaca catatacaaa caaaattttg ttagtcaata attatcacca aactggggtt
     5281 ataacaaggc ttttggatac ttgtgcttgt tgatgttcta ggttcgtatg ataacaaagt
     5341 acatccgtta tatatattcg aaacacactt taatattaaa aatatatatc caattttctt
     5401 gtgaaattta gattatttgg aattaaacct atttctcttg tcttggccac ttgaccggtt
     5461 tagtttttta gacgtatttt attatttctg tttagaaaat aacaacatat atcaactgat
     5521 tttttacttc caatctcttt ttgtcagcac acaaatagaa aaacgtctgt aagctaagct
     5581 atcaactaaa acattaacat atataatctt ttacgttgat agaaaataaa cataaatttc
     5641 tgagttattt tttttttggt tggtgtgtca ctacttactt actactatac ctttttaaca
     5701 ataaagaaac actatttctt tttctattca atataatata tgttttctat ttgtataaat
     5761 ccattacctt tgtttgtttt ataccaaatg ttctttatat ataagtatat gccaccttac
     5821 tctattgaag tcaagaacat atcaaaaacc catgcaagaa gctcacagag aaagaccaaa
     5881 cacttttgtc tctttcttca aaacttttac atatgatctt tgcctctttt cctacaatgg
     5941 gttttgcata actttcacca aaaccctcct caaaagccct tcacatattc ccaacacaag
     6001 aaaataaact ctaaatccac tttcaccaaa tcttttcatt tttcagctaa aataacatga
     6061 gaccgaattc tcgccctata gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga
     6121 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6181 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6241 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     6301 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     6361 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6421 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6481 acgcgcga
//
