LOCUS       sequence-219969-        9958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of hICN1, e.g. for hedgehog-induction. Based
            on addgene #17623, with CMV.GFP removed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: EF.hICN1
FEATURES             Location/Qualifiers
     source          1..9958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     LTR             3778..4011
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4083..4204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4120..4139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4174..4193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4244..4379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4400..4418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4428..4445)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4428..4444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4437..4459)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4586..5041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4673..4692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4883..4904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5067..5171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5172..5240,5241..6032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5390..5409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6203..6791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6692..6711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6945..6962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7079..7100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7115..7120,7121..7138,7139..7145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7153..7169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7158..7180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7177..7193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7177..7193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7212..7232
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7214..7232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7260..7486
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7487..7667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7714..7839
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9109..9342
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9527..9571
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt gatgatccgg aggcatgcgg
     1261 cggcagcatg gccagctctg gttccctgag ggcttcaaag tgtctgaggc cagcaagaag
     1321 aagcggcggg agcccctcgg cgaggactcc gtgggcctca agcccctgaa gaacgcttca
     1381 gacggtgccc tcatggacga caaccagaat gagtgggggg acgaggacct ggagaccaag
     1441 aagttccggt tcgaggagcc cgtggttctg cctgacctgg acgaccagac agaccaccgg
     1501 cagtggactc agcagcacct ggatgccgct gacctgcgca tgtctgccat ggcccccaca
     1561 ccgccccagg gtgaggttga cgccgactgc atggacgtca atgtccgcgg gcctgatggc
     1621 ttcaccccgc tcatgatcgc ctcctgcagc gggggcggcc tggagacggg caacagcgag
     1681 gaagaggagg acgcgccggc cgtcatctcc gacttcatct accagggcgc cagcctgcac
     1741 aaccagacag accgcacggg cgagaccgcc ttgcacctgg ccgcccgcta ctcacgctct
     1801 gattccgcca agcgcctgct ggaggccagc gcagatgcca acatccagga caacatgggc
     1861 cgcaccccgc tgcatgcggc tgtgtctgcc gacgcacaag gtgtcttcca gatcctgatc
     1921 cggaaccgag ccacagacct ggatgcccgc atgcatgatg gcacgacgcc actgatcctg
     1981 gctgcccgcc tggccgtgga gggcatgctg gaggacctca tcaactcaca cgccgacgtc
     2041 aacgccgtag atgacctggg caagtccgcc ctgcactggg ccgccgccgt gaacaatgtg
     2101 gatgccgcag ttgtgctcct gaagaacggg gctaacaaag atatgcagaa caacagggag
     2161 gagacacccc tgtttctggc cgcccgggag ggcagctacg agaccgccaa ggtgctgctg
     2221 gaccactttg ccaaccggga catcacggat catatggacc gcctgccgcg cgacatcgca
     2281 caggagcgca tgcatcacga catcgtgagg ctgctggacg agtacaacct ggtgcgcagc
     2341 ccgcagctgc acggagcccc gctggggggc acgcccaccc tgtcgccccc gctctgctcg
     2401 cccaacggct acctgggcag cctcaagccc ggcgtgcagg gcaagaaggt ccgcaagccc
     2461 agcagcaaag gcctggcctg tggaagcaag gaggccaagg acctcaaggc acggaggaag
     2521 aagtcccagg atggcaaggg ctgcctgctg gacagctccg gcatgctctc gcccgtggac
     2581 tccctggagt caccccatgg ctacctgtca gacgtggcct cgccgccact gctgccctcc
     2641 ccgttccagc agtctccgtc cgtgcccctc aaccacctgc ctgggatgcc cgacacccac
     2701 ctgggcatcg ggcacctgaa cgtggcggcc aagcccgaga tggcggcgct gggtgggggc
     2761 ggccggctgg cctttgagac tggcccacct cgtctctccc acctgcctgt ggcctctggc
     2821 accagcaccg tcctgggctc cagcagcgga ggggccctga atttcactgt gggcgggtcc
     2881 accagtttga atggtcaatg cgagtggctg tcccggctgc agagcggcat ggtgccgaac
     2941 caatacaacc ctctgcgggg gagtgtggca ccaggccccc tgagcacaca ggccccctcc
     3001 ctgcagcatg gcatggtagg cccgctgcac agtagccttg ctgccagcgc cctgtcccag
     3061 atgatgagct accagggcct gcccagcacc cggctggcca cccagcctca cctggtgcag
     3121 acccagcagg tgcagccaca aaacttacag atgcagcagc agaacctgca gccagcaaac
     3181 atccagcagc agcaaagcct gcagccgcca ccaccaccac cacagccgca ccttggcgtg
     3241 agctcagcag ccagcggcca cctgggccgg agcttcctga gtggagagcc gagccaggca
     3301 gacgtgcagc cactgggccc cagcagcctg gcggtgcaca ctattctgcc ccaggagagc
     3361 cccgccctgc ccacgtcgct gccatcctcg ctggtcccac ccgtgaccgc agcccagttc
     3421 ctgacgcccc cctcgcagca cagctactcc tcgcctgtgg acaacacccc cagccaccag
     3481 ctacaggtgc ctgagcaccc cttcctcacc ccgtcccctg agtcccctga ccagtggtcc
     3541 agctcgtccc cgcattccaa cgtctccgac tggtccgagg gcgtctccag ccctcccacc
     3601 agcatgcagt cccagatcgc ccgcattccg gaggccttca agtaaacggc gcgtcagatc
     3661 cactagtaac ggccgccagt gtgctggaat tatcgaattc gagctcggta cctttaagac
     3721 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3781 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     4021 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     4081 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4141 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4201 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     4261 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4321 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4381 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     4441 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4501 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4561 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4621 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4681 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4741 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4801 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4861 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4921 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4981 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5041 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5101 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5161 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5221 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5281 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5341 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5401 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5461 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5521 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5581 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5641 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5701 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5761 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5821 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5881 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5941 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6001 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6061 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6121 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6181 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6241 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6301 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6361 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6421 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6481 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6541 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6601 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6661 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6721 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6781 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6841 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6901 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6961 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7021 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7081 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7141 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7201 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7261 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     7321 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     7381 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     7441 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     7501 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7561 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7621 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7681 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7741 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7801 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7861 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7921 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7981 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8041 gaagaactta gatcattata taatacagta gcaaccctct attgtgtggg taatgactcc
     8101 aacttactga tagtgtttta tgttcagata atgcccgatg accttgtcat gcaactccac
     8161 cgattttgag aacgacagta acttccgtcc cagccttgcc agatgttgtc tcagattcag
     8221 attatgtcgc tcaatgcgct gagtgtaacg cttgctgata acgtgcagct ttcccttcag
     8281 gcgtgattca tacagcggcc agccatccgt catctatacc acgacctcaa aggccgacag
     8341 caggctcaga agacgctcca gtgtggccag agtgcgttca ccgaagacgt gcgccacaac
     8401 cgtcctccgt atcctgtcat acgcgtaaaa cagccagcgc tgacgtgatt tagcaccgac
     8461 gtagccccac tgttcgtcca tttcagcgca gacaatcaca tcactgcccg gttgtatgcg
     8521 cgaggttacc gactgcggcc tgagtttttt aagtgacgta aaaccgtgtt gaggccaacg
     8581 cccataatgc gtgcactggc gcgacatccg acgccattca tggccatatc aatgattttc
     8641 tggtgcttac cgggctgaga ggcggtgtaa gtgaactgta gttgccatgt tttacggcaa
     8701 ggagagcaga gatagcgctg atgtccggca gtgcttttgc cgttacgcac cacgccttca
     8761 gtagcggagc aggaaggaca tctgatggaa atggaagcca cgcaagcacc ttaaaatcac
     8821 catcatacac taaatcagta agttggcagc attacctatt gtgtgcatca aaggatagag
     8881 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8941 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     9001 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     9061 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     9121 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     9181 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     9241 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9301 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9361 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9421 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9481 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     9541 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9601 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9661 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     9721 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     9781 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga
     9841 ctagcctcga gaagcttgat gacggtatcg ataagctttg caaagatgga taaagtttta
     9901 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
