LOCUS       sequence-219986-        9187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral backbone for Gateway cloning of Promoter/Gene insert 
            together with constitutive Blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9187)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWX-R4DESTR2-PGK-BSD
FEATURES             Location/Qualifiers
     source          1..9187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(84..100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(84..100)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(97..119)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    108..124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(132..138,139..156,157..162))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    177..198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        257..452
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      303..438
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    458..592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(495..514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     549..568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(660..677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(831..1418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(911..930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1589..2380,2381..2449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2212..2231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2450..2554)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2585..2964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             2966..3223
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3270..3395
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3887..4004
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4364..4597
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4782..4826
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    5101..5224
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5249..5254,5255..5272,5273..5279)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5333..5992
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5399..5418)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6334..6639
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6518..6537
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6694..6818)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6835..7345
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7264..7282
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      7360..7369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7366..7764
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7783..8371
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7836..7856)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8254..8265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8374..8390)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8375..8391)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(8519..8552)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8572..8752
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8790..8809)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8855..8910
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8909..8928)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
       61 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      121 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      181 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      241 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      301 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      361 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
      421 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
      481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
      601 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      661 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      721 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      781 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      841 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      901 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      961 ccgaccctgc cgcttacgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1021 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1081 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1141 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1201 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1261 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1321 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1381 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1441 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1501 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1561 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1621 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1681 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1741 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1801 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1861 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1921 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1981 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2041 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2101 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2161 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2221 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2281 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2341 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2401 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2461 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2521 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2581 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     2641 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2701 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2761 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2821 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2881 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2941 gtattagtca tcgctattac catgggaggc gtggcctggg cgggactggg gagtggcgag
     3001 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     3061 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     3121 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     3181 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     3241 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     3301 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     3361 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     3421 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     3481 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tactggcctg ttagaaacat
     3541 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     3601 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     3661 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagaaaa
     3721 aagcacagca agcagcagga tcttcagacc tggaaattcc ctacaatccc caaagtcaag
     3781 gagtagtaga atctatgaat aaagaattaa agaaaattat aggacaggta agagatcagg
     3841 ctgaacatct taagacagca gtacaaatgg cagtattcat ccacaatttt aaaagaaaag
     3901 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     3961 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     4021 acagcagaaa tccactttgg aaaggaccag caaagctcct ctggaaaggt gaaggggcag
     4081 tagtaataca agataatagt gacataaaag tagtgccaag aagaaaagca aagatcatta
     4141 gggattatgg aaaacagatg gcaggtgatg attgtgtggc aagtagacag gatgaggatt
     4201 agaacatgga aaagtttagt aaaacaccat aaggaggaga tatgagggac aattggagaa
     4261 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     4321 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     4381 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     4441 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     4501 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     4561 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     4621 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     4681 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     4741 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     4801 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     4861 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     4921 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     4981 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     5041 acagagacag atccattcga ttagtgaacg gatctcgacg gtatctagtg attatcggat
     5101 caactttgta tagaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     5161 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     5221 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
     5281 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
     5341 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     5401 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     5461 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     5521 tgcccgcctg atgaatgctc atccggaact ccgtatggca atgaaagacg gtgagctggt
     5581 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     5641 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     5701 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     5761 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     5821 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     5881 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     5941 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg
     6001 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     6061 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     6121 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     6181 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     6241 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     6301 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     6361 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     6421 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     6481 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     6541 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     6601 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     6661 agccagtctg caggtcgagc agatctgctc gaccatagtg actggatatg ttgtgtttta
     6721 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     6781 ttttacgttt ctcgttcagc tttcttgtac aaagtggtga taatcgaatt ccacggggtt
     6841 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
     6901 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
     6961 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
     7021 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
     7081 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
     7141 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
     7201 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
     7261 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
     7321 ccgaatcacc gacctctctc cccaggggga tccaccggtg ccaccatggc caagcctttg
     7381 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     7441 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     7501 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     7561 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     7621 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     7681 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     7741 tctggttatg tgtgggaggg ctaagatatc aagcttatcg ataatcaacc tctggattac
     7801 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7861 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7921 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7981 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8041 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8101 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8161 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8221 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8281 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8341 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg aggttcgctt
     8401 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8461 gactggaagg gctaattcac tcccaacgaa gacaagatgg gatcaattca ccatgggaat
     8521 aacttcgtat agcatacatt atacgaagtt atgctgcttt ttgcttgtac tgggtctctc
     8581 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8641 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8701 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     8761 ttgaatcgcc agtgtgtcga gctagcagat ctttttccct ctgccaaaaa ttatggggac
     8821 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     8881 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     8941 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     9001 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     9061 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     9121 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     9181 ttcctcc
//
