LOCUS       sequence-22028-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler 
            GR, Copeland TD, Kalkum M, Ge K
  TITLE     PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 
            methyltransferase complex.
  JOURNAL   J Biol Chem. 2007 Jul 13. 282(28):20395-406.
  PUBMED    17500065
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jul 13. 282(28):20395-406."
COMMENT     Sequence Label: pcDNA3 Flag WDR5
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..137
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tggcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat cctggccacc atggactaca
      121 aggacgacga tgacaagatg gccacagagg agaagaagcc agagacagag gctgcaagag
      181 cacagcccac tccttcctca tcagccacac agagcaagcc cacaccagtt aagccaaact
      241 atgccctgaa gttcaccctg gctggccaca ccaaagctgt gtcctctgtg aagttcagcc
      301 ccaatgggga atggttggca agttcatctg ctgataaact cattaaaatt tggggagcat
      361 atgatggaaa gtttgagaaa actatatctg gtcacaaact gggaatatct gatgtagcgt
      421 ggtcatcaga ttctaacctc cttgtgtctg cctctgatga taaaactttg aagatttggg
      481 acgtgagttc cgggaagtgt ctgaagaccc tgaagggcca cagtaactac gtcttctgct
      541 gcaacttcaa cccccagtcc aacctcatcg tctcagggtc ttttgatgaa agtgtgagga
      601 tatgggacgt gaagacaggg aagtgcctca agactttgcc tgcccattcg gacccagtct
      661 cagccgttca tttcaaccgt ga
//
