LOCUS       sequence-22033-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler 
            GR, Copeland TD, Kalkum M, Ge K
  TITLE     PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 
            methyltransferase complex.
  JOURNAL   J Biol Chem. 2007 Jul 13. 282(28):20395-406.
  PUBMED    17500065
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jul 13. 282(28):20395-406."
COMMENT     Sequence Label: pcDNA3 Flag mPTIP
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctacta gagaacccct gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttggtaccga gctcggatct tggccaccat ggactacaag
      121 gacgacgatg acaagatgtc ggagccggcg cccgaagttc cagaggagct attccgcgag
      181 gtcaagtact acgcggtggg cgacatcgac ccgcaggtta ttcagctcct taaagctggg
      241 aaagccaagg aagtctccta caatgccctg gcctcacaca tcatttcaga ggatggggac
      301 aacccagagg tgggagaagc ccgtgaggtc tttgacttgc ccgttgtaaa gccttcctgg
      361 gtgactctgt ccgtgcaatg tggcgctctc ctgccagtca atggtttctc tccagagtcc
      421 tgtcagattt tttttggact cactgcctgc ctttctcagg tgtcatctga agacagaagt
      481 gccctgtggg ccttggttac gttccacggg ggcagttgcc aactaaacct taacaagaag
      541 tgcacacact tgattgtccc agagccaaag ggggagaaat acgagcgtgc tgtcaaaaga
      601 acaagtatta agattgtcac cccggactgg gtcctggact gtgtgtctga gaaga
//
