LOCUS       sequence-220890-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes ConLS' as a Type 1 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK008
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(517..536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcac gttgtattgc gacgaattgc cacgttgtcg
      181 cagtgtctgt actgtgggtt aatgtctgtg atcgccgtcg tgagttcgtg tcatccgcgc
      241 atgattcaga tggtcctgga gatcgttgct tgtaccggct gtttgtggct ctaccacgtc
      301 tcatcagtct agatgcgaat tcagggtgag accgactacg gttatccaca gaatcagggg
      361 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      421 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      481 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      541 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      601 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      661 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      721 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      781 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      841 tcttgaagtg gtggcctaac tacggctaca ctagaa
//
